TY - JOUR
T1 - K3 killer yeast is a mutant K2 killer yeast
AU - Wingfield, Brenda D.
AU - Van Der Meer, Linda J.
AU - Pretorius, Isak S.
AU - Van Vuuren, Hendrik J J
PY - 1990
Y1 - 1990
N2 - The K2 and K3 killer yeast classes in Saccharomyces cerevisiae were compared. Sensitive strain 381 and the killer strains NCYC 232 NCYC 190, NCYC 738, NCYC 761 and KT28 were sensitive to the killer toxins produced by the K2 and K3 strains. However, these two strains were resistant to both toxins. Hybridization analysis showed that the M dsRNAs from the K2 and K3 strains had significant homology, but shared no homology with the dsRNAs from the K1 and KT28 strains. The K2 M dsRNA is larger than the K3 M dsRNA, and electron micrographs showed an unmatched loop when these molecules were hybridized. This suggests that the K3 M dsRNA is a mutant K2 M genome. These results show that K2 and K3 strains belong in the same class, but separate from the K1 and KT28 killer ty
AB - The K2 and K3 killer yeast classes in Saccharomyces cerevisiae were compared. Sensitive strain 381 and the killer strains NCYC 232 NCYC 190, NCYC 738, NCYC 761 and KT28 were sensitive to the killer toxins produced by the K2 and K3 strains. However, these two strains were resistant to both toxins. Hybridization analysis showed that the M dsRNAs from the K2 and K3 strains had significant homology, but shared no homology with the dsRNAs from the K1 and KT28 strains. The K2 M dsRNA is larger than the K3 M dsRNA, and electron micrographs showed an unmatched loop when these molecules were hybridized. This suggests that the K3 M dsRNA is a mutant K2 M genome. These results show that K2 and K3 strains belong in the same class, but separate from the K1 and KT28 killer ty
KW - dsRNA
KW - Killer yeast
KW - Saccharomyces cerevisiae
UR - http://www.scopus.com/inward/record.url?scp=0008892955&partnerID=8YFLogxK
U2 - 10.1016/S0953-7562(09)81304-X
DO - 10.1016/S0953-7562(09)81304-X
M3 - Article
AN - SCOPUS:0008892955
SN - 0953-7562
VL - 94
SP - 901
EP - 906
JO - Mycological Research
JF - Mycological Research
IS - 7
ER -