Kynurenine pathway metabolism is involved in the maintenance of the intracellular NAD + concentration in human primary astrocytes

Ross Grant*, Susan Nguyen, Gilles Guillemin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)
7 Downloads (Pure)

Abstract

Efficient synthesis of NAD + is critical to maintaining cell viability in all organs of the body. However, little is known of the pathway(s) by which cells of the central nervous system produce NAD +. The aim of this study was to investigate the relationship, between tryptophan degradation via the kynurenine pathway (KP) and de novo NAD + synthesis in human astrocytes, a major cell type within the brain. In this study we observed that inhibition of single enzymes of the KP resulted in significant decreases in NAD + levels in astroglial cells after a 24 hr period. We also observed that astrocytes cultured in media deficient in tryptophan, nicotinic acid and nicotinamide resulted in a 50% decrease in NAD + levels after 24 hrs. This decrease in NAD + was partially restored by supplementation of the culture media with either tryptophan or kynurenine, or nicotinic acid or with supply of the salvage pathway precursor nicotinamide.

Original languageEnglish
Pages (from-to)151-156
Number of pages6
JournalInternational Journal of Tryptophan Research
Volume3
Issue number1
Publication statusPublished - 2010
Externally publishedYes

Bibliographical note

Copyright the Author(s) 2010. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

  • Astrocyte
  • NAD
  • Nicotinamide
  • Nicotnic acid
  • Tryptophan

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