Label-free assessment of endothelial cell metabolic state using autofluorescent microscopy

Benjamin J. Pullen, Tam Nguyen, Martin Gosnell, Ayad G. Anwer, Ewa Goldys, Stephen J. Nicholls, Peter J. Psaltis

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contribution


To examine the process of endothelial cell aging we utilised hyperspectral imaging to collect broad autofluorescence emission at the individual cellular level and mathematically isolate the characteristic spectra of nicotinamide and flavin adenine dinucleotides (NADH and FAD, respectively). Quantitative analysis of this data provides the basis for a non-destructive spatial imaging method for cells and tissue. FAD and NADH are important factors in cellular metabolism and have been shown to be involved with the redox state of the cell; with the ratio between the two providing the basis for an ‘optical redox ratio’.
Original languageEnglish
Title of host publicationProceedings of SPIE
Subtitle of host publicationSPIE BioPhotonics Australasia
EditorsMark R. Hutchinson, Ewa M. Goldys
Place of PublicationBellingham, Washington
Number of pages1
ISBN (Electronic)9781510604353
ISBN (Print)9781510604346
Publication statusPublished - 9 Dec 2016
EventSPIE BioPhotonics Australasia - Adelaide, Australia
Duration: 17 Oct 201619 Oct 2016

Publication series

NameProceedings of SPIE
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X


OtherSPIE BioPhotonics Australasia


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