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Label-free, non-invasive multispectral imaging of cellular autofluorescence for melanoma detection

Aline Knab*, Ayad G. Anwer, Bernadette Pedersen, Shannon Handley, Abhilash Goud Marupally, Abbas Habibalahi, Ewa M. Goldys

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contributionpeer-review

Abstract

Melanoma, the most invasive skin cancer, has high mortality rates for advanced cases despite treatment advancements. This study explores the potential of multispectral autofluorescence imaging as a diagnostic tool for melanoma. We assessed autofluorescent signatures from cultured immortalized melanoma cell lines and fibroblasts, using feature analysis to evaluate image data. The analysis revealed highly distinctive cell groups based on autofluorescence characteristics. Additionally, we investigated the role of autofluorescence intensity features in simulating real-world conditions by evaluating unprocessed, background-corrupted data. Our findings demonstrate the efficacy of this labelfree imaging approach for melanoma detection, highlighting its promising clinical potential.

Original languageEnglish
Title of host publicationPhotonics in Dermatology and Plastic Surgery 2025
EditorsHaishan Zeng, Milind Rajadhyaksha
Place of PublicationWashington
PublisherSPIE
Pages1-5
Number of pages5
ISBN (Electronic)9781510683334
ISBN (Print)9781510683327
DOIs
Publication statusPublished - 2025
EventPhotonics in Dermatology and Plastic Surgery 2025 - San Francisco, United States
Duration: 25 Jan 202527 Jan 2025

Publication series

NameProgress in Biomedical Optics and Imaging
PublisherSPIE
Volume13292
ISSN (Print)1605-7422
ISSN (Electronic)2410-9045

Conference

ConferencePhotonics in Dermatology and Plastic Surgery 2025
Country/TerritoryUnited States
CitySan Francisco
Period25/01/2527/01/25

Keywords

  • autofluorescence
  • feature analysis
  • fibroblasts
  • label-free
  • machine learning
  • melanoma
  • multispectral

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