Label-free, zeptomole cancer biomarker detection by surface-enhanced fluorescence on nanoporous gold disk plasmonic nanoparticles

Greggy M. Santos; Fusheng Zhao; Jianbo Zeng; Ming Li; Wei-Chuan Shih

doi:10.1002/jbio.201400134

Label-free, zeptomole cancer biomarker detection by surface-enhanced fluorescence on nanoporous gold disk plasmonic nanoparticles

Greggy M. Santos, Fusheng Zhao, Jianbo Zeng, Ming Li, Wei-Chuan Shih

Research output: Contribution to journal › Article › peer-review

48 Citations (Scopus)

Abstract

We experimentally demonstrate a label‐free biosensor for the ERBB2 cancer gene DNA target based on the distance‐dependent detection of surface‐enhanced fluorescence (SEF) on nanoporous gold disk (NPGD) plasmonic nanoparticles. We achieve detection of 2.4 zeptomole of DNA target on the NPGD substrate with an upper concentration detection limit of 1 nM. Without the use of molecular spacers, the NPGD substrate as an SEF platform was shown to provide higher net fluorescence for visible and NIR fluorophores compared to glass and non‐porous gold substrates. The enhanced fluorescence signals in patterned nanoporous gold nanoparticles make NPGD a viable material for further reducing detection limits for biomolecular targets used in clinical assays.

Original language	English
Pages (from-to)	855-863
Number of pages	9
Journal	Journal of Biophotonics
Volume	8
Issue number	10
DOIs	https://doi.org/10.1002/jbio.201400134
Publication status	Published - Oct 2015
Externally published	Yes

Keywords

surface-enhanced fluorescence
nanoporous gold disks
label-free biosensor
zeptomole detection
molecular beacon
Zeptomole detection
Nanoporous gold disks
Molecular beacon
Surface-enhanced fluorescence
Label-free biosensor

Access to Document

10.1002/jbio.201400134

Cite this

@article{a3b5117ad257455a8a50b9eaf3d477d5,

title = "Label-free, zeptomole cancer biomarker detection by surface-enhanced fluorescence on nanoporous gold disk plasmonic nanoparticles",

abstract = "We experimentally demonstrate a label‐free biosensor for the ERBB2 cancer gene DNA target based on the distance‐dependent detection of surface‐enhanced fluorescence (SEF) on nanoporous gold disk (NPGD) plasmonic nanoparticles. We achieve detection of 2.4 zeptomole of DNA target on the NPGD substrate with an upper concentration detection limit of 1 nM. Without the use of molecular spacers, the NPGD substrate as an SEF platform was shown to provide higher net fluorescence for visible and NIR fluorophores compared to glass and non‐porous gold substrates. The enhanced fluorescence signals in patterned nanoporous gold nanoparticles make NPGD a viable material for further reducing detection limits for biomolecular targets used in clinical assays.",

keywords = "surface-enhanced fluorescence, nanoporous gold disks, label-free biosensor, zeptomole detection, molecular beacon, Zeptomole detection, Nanoporous gold disks, Molecular beacon, Surface-enhanced fluorescence, Label-free biosensor",

author = "Santos, {Greggy M.} and Fusheng Zhao and Jianbo Zeng and Ming Li and Wei-Chuan Shih",

year = "2015",

month = oct,

doi = "10.1002/jbio.201400134",

language = "English",

volume = "8",

pages = "855--863",

journal = "Journal of Biophotonics",

issn = "1864-063X",

publisher = "WILEY-VCH Verlag Weinheim",

number = "10",

}

TY - JOUR

T1 - Label-free, zeptomole cancer biomarker detection by surface-enhanced fluorescence on nanoporous gold disk plasmonic nanoparticles

AU - Santos, Greggy M.

AU - Zhao, Fusheng

AU - Zeng, Jianbo

AU - Li, Ming

AU - Shih, Wei-Chuan

PY - 2015/10

Y1 - 2015/10

N2 - We experimentally demonstrate a label‐free biosensor for the ERBB2 cancer gene DNA target based on the distance‐dependent detection of surface‐enhanced fluorescence (SEF) on nanoporous gold disk (NPGD) plasmonic nanoparticles. We achieve detection of 2.4 zeptomole of DNA target on the NPGD substrate with an upper concentration detection limit of 1 nM. Without the use of molecular spacers, the NPGD substrate as an SEF platform was shown to provide higher net fluorescence for visible and NIR fluorophores compared to glass and non‐porous gold substrates. The enhanced fluorescence signals in patterned nanoporous gold nanoparticles make NPGD a viable material for further reducing detection limits for biomolecular targets used in clinical assays.

AB - We experimentally demonstrate a label‐free biosensor for the ERBB2 cancer gene DNA target based on the distance‐dependent detection of surface‐enhanced fluorescence (SEF) on nanoporous gold disk (NPGD) plasmonic nanoparticles. We achieve detection of 2.4 zeptomole of DNA target on the NPGD substrate with an upper concentration detection limit of 1 nM. Without the use of molecular spacers, the NPGD substrate as an SEF platform was shown to provide higher net fluorescence for visible and NIR fluorophores compared to glass and non‐porous gold substrates. The enhanced fluorescence signals in patterned nanoporous gold nanoparticles make NPGD a viable material for further reducing detection limits for biomolecular targets used in clinical assays.

KW - surface-enhanced fluorescence

KW - nanoporous gold disks

KW - label-free biosensor

KW - zeptomole detection

KW - molecular beacon

KW - Zeptomole detection

KW - Nanoporous gold disks

KW - Molecular beacon

KW - Surface-enhanced fluorescence

KW - Label-free biosensor

UR - http://www.scopus.com/inward/record.url?scp=84945454043&partnerID=8YFLogxK

U2 - 10.1002/jbio.201400134

DO - 10.1002/jbio.201400134

M3 - Article

C2 - 25727212

VL - 8

SP - 855

EP - 863

JO - Journal of Biophotonics

JF - Journal of Biophotonics

SN - 1864-063X

IS - 10

ER -

Label-free, zeptomole cancer biomarker detection by surface-enhanced fluorescence on nanoporous gold disk plasmonic nanoparticles

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this