Label-free, zeptomole cancer biomarker detection by surface-enhanced fluorescence on nanoporous gold disk plasmonic nanoparticles

Greggy M. Santos, Fusheng Zhao, Jianbo Zeng, Ming Li, Wei-Chuan Shih

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

We experimentally demonstrate a label‐free biosensor for the ERBB2 cancer gene DNA target based on the distance‐dependent detection of surface‐enhanced fluorescence (SEF) on nanoporous gold disk (NPGD) plasmonic nanoparticles. We achieve detection of 2.4 zeptomole of DNA target on the NPGD substrate with an upper concentration detection limit of 1 nM. Without the use of molecular spacers, the NPGD substrate as an SEF platform was shown to provide higher net fluorescence for visible and NIR fluorophores compared to glass and non‐porous gold substrates. The enhanced fluorescence signals in patterned nanoporous gold nanoparticles make NPGD a viable material for further reducing detection limits for biomolecular targets used in clinical assays.
Original languageEnglish
Pages (from-to)855-863
Number of pages9
JournalJournal of Biophotonics
Volume8
Issue number10
DOIs
Publication statusPublished - Oct 2015
Externally publishedYes

Keywords

  • surface-enhanced fluorescence
  • nanoporous gold disks
  • label-free biosensor
  • zeptomole detection
  • molecular beacon
  • Zeptomole detection
  • Nanoporous gold disks
  • Molecular beacon
  • Surface-enhanced fluorescence
  • Label-free biosensor

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