Labeling proteins with small molecules by site-specific posttranslational modification

Jun Yin, Fei Liu, Xiaohua Li, Christopher T. Walsh*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

191 Citations (Scopus)


We report here the development of a general strategy for site-specific labeling of proteins with small molecules by posttranslational modification enzyme, phosphopantetheinyl transferase Sfp. The target proteins are expressed as fusions to the peptide carrier protein (PCP) excised from nonribosomal peptide synthetase, and Sfp catalyzes the covalent modification of a specific serine residue on PCP by the small molecule-phosphopantetheinyl conjugate. The labeling reaction proceeds with high specificity and efficiency, targeting PCP fusion proteins in the cell lysate. The PCP tag has been shown to be compatible with various proteins, and Sfp-catalyzed PCP modification, compatible with various small-molecule probes conjugated to coenzyme A, highlighting the potential of the PCP tag for site-specific protein labeling with small molecules.

Original languageEnglish
Pages (from-to)7754-7755
Number of pages2
JournalJournal of the American Chemical Society
Issue number25
Publication statusPublished - 30 Jun 2004
Externally publishedYes


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