Laser oblique scanning optical microscopy (LOSOM) for phase relief imaging

Yichen Ding; Hao Xie; Tong Peng; Yiqing Lu; Dayong Jin; Junlin Teng; Qiushi Ren; Peng Xi

doi:10.1364/OE.20.014100

Laser oblique scanning optical microscopy (LOSOM) for phase relief imaging

Yichen Ding^*, Hao Xie, Tong Peng, Yiqing Lu, Dayong Jin, Junlin Teng, Qiushi Ren, Peng Xi

^*Corresponding author for this work

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Abstract

The visualization of optical phase can provide abundant information when imaging transparent specimen. We present a novel phase sensitive imaging design capable of obtaining phase contours of transparent biological cells through laser oblique scanning optical microscope (LOSOM). LOSOM is based on the introduction of a fluorescent medium behind the specimen to generate a differential phase-sensitive image, thus, the complicated phase retardation alignment procedure associated with differential interference contrast (DIC) microscopy can be eliminated. Moreover, multi-modality fluorescence and phase relief imaging can be attained in a single system with fluorescently labeled specimens.

Original language	English
Pages (from-to)	14100-14108
Number of pages	9
Journal	Optics Express
Volume	20
Issue number	13
DOIs	https://doi.org/10.1364/OE.20.014100
Publication status	Published - 18 Jun 2012

Bibliographical note

This paper was published in Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: [http://www.opticsinfobase.org/oe/abstract.cfm?uri=oe-20-13-14100]. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.

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abstract = "The visualization of optical phase can provide abundant information when imaging transparent specimen. We present a novel phase sensitive imaging design capable of obtaining phase contours of transparent biological cells through laser oblique scanning optical microscope (LOSOM). LOSOM is based on the introduction of a fluorescent medium behind the specimen to generate a differential phase-sensitive image, thus, the complicated phase retardation alignment procedure associated with differential interference contrast (DIC) microscopy can be eliminated. Moreover, multi-modality fluorescence and phase relief imaging can be attained in a single system with fluorescently labeled specimens.",

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doi = "10.1364/OE.20.014100",

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AU - Teng, Junlin

AU - Ren, Qiushi

AU - Xi, Peng

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Laser oblique scanning optical microscopy (LOSOM) for phase relief imaging

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