Abstract
Lectins are a group of proteins that bind specifically and reversibly to mono- and oligosaccharide carbohydrate structures that are present on the surfaces of mammalian cells. The use of lectins as capture agents in microfluidic channels was examined with a focus on cells associated with T and B lymphocytic leukemia. In addition to examining the adhesion of Jurkat T and Raji B lymphocytes to a broad panel of lectins, this work also examined the capture of these cells from whole blood. Captured T and B lymphocytes were eluted from the microfluidic devices with a solution of the lectin's inhibiting sugar. The capture and release steps were accomplished in under 1 h. The significance of this work lies within the realm of low-cost capture of abundant target cells with non-stimulatory elution capability.
Original language | English |
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Pages (from-to) | 565-571 |
Number of pages | 7 |
Journal | Biomedical Microdevices |
Volume | 13 |
Issue number | 3 |
DOIs | |
Publication status | Published - Jun 2011 |
Keywords
- Blood
- Cell adhesion
- Cell capture
- Lectins
- Leukemia
- Microfluidics