Less label, more free: Approaches in label-free quantitative mass spectrometry

Karlie A. Neilson, Naveid A. Ali, Sridevi Muralidharan, Mehdi Mirzaei, Michael Mariani, Gariné Assadourian, Albert Lee, Steven C. Van Sluyter, Paul A. Haynes

Research output: Contribution to journalReview articleResearchpeer-review

Abstract

In this review we examine techniques, software, and statistical analyses used in label-free quantitative proteomics studies for area under the curve and spectral counting approaches. Recent advances in the field are discussed in an order that reflects a logical workflow design. Examples of studies that follow this design are presented to highlight the requirement for statistical assessment and further experiments to validate results from label-free quantitation. Limitations of label-free approaches are considered, label-free approaches are compared with labelling techniques, and forward-looking applications for label-free quantitative data are presented. We conclude that label-free quantitative proteomics is a reliable, versatile, and cost-effective alternative to labelled quantitation.

LanguageEnglish
Pages535-553
Number of pages19
JournalProteomics
Volume11
Issue number4
DOIs
Publication statusPublished - Feb 2011

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Proteomics
Mass spectrometry
Labels
Mass Spectrometry
Workflow
Area Under Curve
Software
Costs and Cost Analysis
Labeling
Costs
Experiments

Cite this

Neilson, Karlie A. ; Ali, Naveid A. ; Muralidharan, Sridevi ; Mirzaei, Mehdi ; Mariani, Michael ; Assadourian, Gariné ; Lee, Albert ; Van Sluyter, Steven C. ; Haynes, Paul A. / Less label, more free : Approaches in label-free quantitative mass spectrometry. In: Proteomics. 2011 ; Vol. 11, No. 4. pp. 535-553.
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Neilson, KA, Ali, NA, Muralidharan, S, Mirzaei, M, Mariani, M, Assadourian, G, Lee, A, Van Sluyter, SC & Haynes, PA 2011, 'Less label, more free: Approaches in label-free quantitative mass spectrometry', Proteomics, vol. 11, no. 4, pp. 535-553. https://doi.org/10.1002/pmic.201000553

Less label, more free : Approaches in label-free quantitative mass spectrometry. / Neilson, Karlie A.; Ali, Naveid A.; Muralidharan, Sridevi; Mirzaei, Mehdi; Mariani, Michael; Assadourian, Gariné; Lee, Albert; Van Sluyter, Steven C.; Haynes, Paul A.

In: Proteomics, Vol. 11, No. 4, 02.2011, p. 535-553.

Research output: Contribution to journalReview articleResearchpeer-review

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Neilson KA, Ali NA, Muralidharan S, Mirzaei M, Mariani M, Assadourian G et al. Less label, more free: Approaches in label-free quantitative mass spectrometry. Proteomics. 2011 Feb;11(4):535-553. https://doi.org/10.1002/pmic.201000553