Leveraging a natural murine meiotic drive to suppress invasive populations

Luke Gierus; Aysegul Birand; Mark D. Bunting; Gelshan I. Godahewa; Sandra G. Piltz; Kevin P. Oh; Antoinette J. Piaggio; David W. Threadgill; John R. Godwin; Owain Edwards; Phillip Cassey; Joshua V. Ross; Thomas A. A. Prowse; Paul Q. Thomas

doi:10.1073/pnas.2213308119

Leveraging a natural murine meiotic drive to suppress invasive populations

Luke Gierus, Aysegul Birand, Mark D. Bunting, Gelshan I. Godahewa, Sandra G. Piltz, Kevin P. Oh, Antoinette J. Piaggio, David W. Threadgill, John R. Godwin, Owain Edwards, Phillip Cassey, Joshua V. Ross, Thomas A. A. Prowse, Paul Q. Thomas^*

^*Corresponding author for this work

Applied BioSciences

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

112 Downloads (Pure)

Abstract

Invasive rodents are a major cause of environmental damage and biodiversity loss, particularly on islands. Unlike insects, genetic biocontrol strategies including population-suppressing gene drives with biased inheritance have not been developed in mice. Here, we demonstrate a gene drive strategy (t_CRISPR) that leverages super-Mendelian transmission of the t haplotype to spread inactivating mutations in a haplosufficient female fertility gene (Prl). Using spatially explicit individual-based in silico modeling, we show that t_CRISPR can eradicate island populations under a range of realistic field-based parameter values. We also engineer transgenic t_CRISPR mice that, crucially, exhibit biased transmission of the modified t haplotype and Prl mutations at levels our modeling predicts would be sufficient for eradication. This is an example of a feasible gene drive system for invasive alien rodent population control.

Original language	English
Article number	e2213308119
Pages (from-to)	1-11
Number of pages	11
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	119
Issue number	46
Early online date	8 Nov 2022
DOIs	https://doi.org/10.1073/pnas.2213308119
Publication status	Published - 15 Nov 2022

Bibliographical note

Copyright the Author(s) 2022. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Correction: Luke Gierus, Aysegul Birand, Mark D. Bunting, Gelshan I. Godahewa, Sandra G. Piltz, Kevin P. Oh, Antoinette J. Piaggio, David W. Threadgill, John Godwin, Owain Edwards, Phillip Cassey, Joshua V. Ross, Thomas A. A. Prowse and Paul Q. Thomas (2022) Leveraging a natural murine meiotic drive to suppress invasive populations, PNAS, 120(2), e2220857120,
https://doi.org/10.1073/pnas.2220857120.

Keywords

genetic biocontro
gene drive
invasive rodents
conservation
modeling

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10.1073/pnas.2213308119

Publisher version (open access)Final published version, 2.26 MBLicence: CC BY-NC-ND

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Gierus, L., Birand, A., Bunting, M. D., Godahewa, G. I., Piltz, S. G., Oh, K. P., Piaggio, A. J., Threadgill, D. W., Godwin, J. R., Edwards, O., Cassey, P., Ross, J. V., Prowse, T. A. A., & Thomas, P. Q. (2022). Leveraging a natural murine meiotic drive to suppress invasive populations. Proceedings of the National Academy of Sciences of the United States of America, 119(46), 1-11. Article e2213308119. https://doi.org/10.1073/pnas.2213308119

@article{a5b1fc8bc39242ff90b03a4b8984f35a,

title = "Leveraging a natural murine meiotic drive to suppress invasive populations",

abstract = "Invasive rodents are a major cause of environmental damage and biodiversity loss, particularly on islands. Unlike insects, genetic biocontrol strategies including population-suppressing gene drives with biased inheritance have not been developed in mice. Here, we demonstrate a gene drive strategy (tCRISPR) that leverages super-Mendelian transmission of the t haplotype to spread inactivating mutations in a haplosufficient female fertility gene (Prl). Using spatially explicit individual-based in silico modeling, we show that tCRISPR can eradicate island populations under a range of realistic field-based parameter values. We also engineer transgenic tCRISPR mice that, crucially, exhibit biased transmission of the modified t haplotype and Prl mutations at levels our modeling predicts would be sufficient for eradication. This is an example of a feasible gene drive system for invasive alien rodent population control.",

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author = "Luke Gierus and Aysegul Birand and Bunting, \{Mark D.\} and Godahewa, \{Gelshan I.\} and Piltz, \{Sandra G.\} and Oh, \{Kevin P.\} and Piaggio, \{Antoinette J.\} and Threadgill, \{David W.\} and Godwin, \{John R.\} and Owain Edwards and Phillip Cassey and Ross, \{Joshua V.\} and Prowse, \{Thomas A. A.\} and Thomas, \{Paul Q.\}",

note = "Copyright the Author(s) 2022. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher. Correction: Luke Gierus, Aysegul Birand, Mark D. Bunting, Gelshan I. Godahewa, Sandra G. Piltz, Kevin P. Oh, Antoinette J. Piaggio, David W. Threadgill, John Godwin, Owain Edwards, Phillip Cassey, Joshua V. Ross, Thomas A. A. Prowse and Paul Q. Thomas (2022) Leveraging a natural murine meiotic drive to suppress invasive populations, PNAS, 120(2), e2220857120, https://doi.org/10.1073/pnas.2220857120.",

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Gierus, L, Birand, A, Bunting, MD, Godahewa, GI, Piltz, SG, Oh, KP, Piaggio, AJ, Threadgill, DW, Godwin, JR, Edwards, O, Cassey, P, Ross, JV, Prowse, TAA & Thomas, PQ 2022, 'Leveraging a natural murine meiotic drive to suppress invasive populations', Proceedings of the National Academy of Sciences of the United States of America, vol. 119, no. 46, e2213308119, pp. 1-11. https://doi.org/10.1073/pnas.2213308119

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T1 - Leveraging a natural murine meiotic drive to suppress invasive populations

AU - Gierus, Luke

AU - Birand, Aysegul

AU - Bunting, Mark D.

AU - Godahewa, Gelshan I.

AU - Piltz, Sandra G.

AU - Oh, Kevin P.

AU - Piaggio, Antoinette J.

AU - Threadgill, David W.

AU - Godwin, John R.

AU - Edwards, Owain

AU - Cassey, Phillip

AU - Ross, Joshua V.

AU - Prowse, Thomas A. A.

AU - Thomas, Paul Q.

N1 - Copyright the Author(s) 2022. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher. Correction: Luke Gierus, Aysegul Birand, Mark D. Bunting, Gelshan I. Godahewa, Sandra G. Piltz, Kevin P. Oh, Antoinette J. Piaggio, David W. Threadgill, John Godwin, Owain Edwards, Phillip Cassey, Joshua V. Ross, Thomas A. A. Prowse and Paul Q. Thomas (2022) Leveraging a natural murine meiotic drive to suppress invasive populations, PNAS, 120(2), e2220857120, https://doi.org/10.1073/pnas.2220857120.

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AB - Invasive rodents are a major cause of environmental damage and biodiversity loss, particularly on islands. Unlike insects, genetic biocontrol strategies including population-suppressing gene drives with biased inheritance have not been developed in mice. Here, we demonstrate a gene drive strategy (tCRISPR) that leverages super-Mendelian transmission of the t haplotype to spread inactivating mutations in a haplosufficient female fertility gene (Prl). Using spatially explicit individual-based in silico modeling, we show that tCRISPR can eradicate island populations under a range of realistic field-based parameter values. We also engineer transgenic tCRISPR mice that, crucially, exhibit biased transmission of the modified t haplotype and Prl mutations at levels our modeling predicts would be sufficient for eradication. This is an example of a feasible gene drive system for invasive alien rodent population control.

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KW - gene drive

KW - invasive rodents

KW - conservation

KW - modeling

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UR - https://doi.org/10.1073/pnas.222085712

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SN - 0027-8424

VL - 119

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EP - 11

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 46

M1 - e2213308119

ER -

Leveraging a natural murine meiotic drive to suppress invasive populations

Abstract

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Keywords

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