Ligand-induced dissociation of the asymmetric homodimer of ristocetin A monitored by 19F NMR spectroscopy

Robert J. Dancer; Andrew C. Try; Dudley H. Williams

doi:10.1002/(SICI)1521-3765(19980416)4:4<740::AID-CHEM740>3.0.CO;2-O

Ligand-induced dissociation of the asymmetric homodimer of ristocetin A monitored by 19F NMR spectroscopy

Robert J. Dancer^*, Andrew C. Try, Dudley H. Williams

^*Corresponding author for this work

Macquarie University BioFocus Research Centre

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

In this paper we demonstrate the use of ¹⁹F-labelled ligands in the study of the therapeutically important vancomycin group of antibiotics. The substantial simplification of spectra that occurs when such labelled ligands are employed is used to study the anticooperativity between ligand binding and dimerisation of ristocetin A. The general utility of ¹⁹F-labelled ligands is demonstrated, with the caveat that the positioning of the label has to be carefully considered. With the growing therapeutic importance of the vancomycin group of glycopeptide antibiotics, such a tool may prove valuable in the detailed study of their mode of action.

Original language	English
Pages (from-to)	740-744
Number of pages	5
Journal	Chemistry - A European Journal
Volume	4
Issue number	4
DOIs	https://doi.org/10.1002/(SICI)1521-3765(19980416)4:4<740::AID-CHEM740>3.0.CO;2-O
Publication status	Published - Apr 1998

Keywords

Antibiotics
Asymmetric homodimer
Cooperative effects
Glycopeptides
NMR spectroscopy

Access to Document

10.1002/(SICI)1521-3765(19980416)4:4<740::AID-CHEM740>3.0.CO;2-O

Link to publication in Scopus

Fingerprint Dive into the research topics of 'Ligand-induced dissociation of the asymmetric homodimer of ristocetin A monitored by 19F NMR spectroscopy'. Together they form a unique fingerprint.

Cite this

@article{5be25e344437446db039bf063dde5dac,

title = "Ligand-induced dissociation of the asymmetric homodimer of ristocetin A monitored by 19F NMR spectroscopy",

abstract = "In this paper we demonstrate the use of 19F-labelled ligands in the study of the therapeutically important vancomycin group of antibiotics. The substantial simplification of spectra that occurs when such labelled ligands are employed is used to study the anticooperativity between ligand binding and dimerisation of ristocetin A. The general utility of 19F-labelled ligands is demonstrated, with the caveat that the positioning of the label has to be carefully considered. With the growing therapeutic importance of the vancomycin group of glycopeptide antibiotics, such a tool may prove valuable in the detailed study of their mode of action.",

keywords = "Antibiotics, Asymmetric homodimer, Cooperative effects, Glycopeptides, NMR spectroscopy",

author = "Dancer, {Robert J.} and Try, {Andrew C.} and Williams, {Dudley H.}",

year = "1998",

month = apr,

doi = "10.1002/(SICI)1521-3765(19980416)4:4<740::AID-CHEM740>3.0.CO;2-O",

language = "English",

volume = "4",

pages = "740--744",

journal = "Chemistry - A European Journal",

issn = "0947-6539",

publisher = "Wiley-VCH, Wiley",

number = "4",

}

TY - JOUR

T1 - Ligand-induced dissociation of the asymmetric homodimer of ristocetin A monitored by 19F NMR spectroscopy

AU - Dancer, Robert J.

AU - Try, Andrew C.

AU - Williams, Dudley H.

PY - 1998/4

Y1 - 1998/4

N2 - In this paper we demonstrate the use of 19F-labelled ligands in the study of the therapeutically important vancomycin group of antibiotics. The substantial simplification of spectra that occurs when such labelled ligands are employed is used to study the anticooperativity between ligand binding and dimerisation of ristocetin A. The general utility of 19F-labelled ligands is demonstrated, with the caveat that the positioning of the label has to be carefully considered. With the growing therapeutic importance of the vancomycin group of glycopeptide antibiotics, such a tool may prove valuable in the detailed study of their mode of action.

AB - In this paper we demonstrate the use of 19F-labelled ligands in the study of the therapeutically important vancomycin group of antibiotics. The substantial simplification of spectra that occurs when such labelled ligands are employed is used to study the anticooperativity between ligand binding and dimerisation of ristocetin A. The general utility of 19F-labelled ligands is demonstrated, with the caveat that the positioning of the label has to be carefully considered. With the growing therapeutic importance of the vancomycin group of glycopeptide antibiotics, such a tool may prove valuable in the detailed study of their mode of action.

KW - Antibiotics

KW - Asymmetric homodimer

KW - Cooperative effects

KW - Glycopeptides

KW - NMR spectroscopy

UR - http://www.scopus.com/inward/record.url?scp=0031804668&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1521-3765(19980416)4:4<740::AID-CHEM740>3.0.CO;2-O

DO - 10.1002/(SICI)1521-3765(19980416)4:4<740::AID-CHEM740>3.0.CO;2-O

M3 - Article

AN - SCOPUS:0031804668

VL - 4

SP - 740

EP - 744

JO - Chemistry - A European Journal

JF - Chemistry - A European Journal

SN - 0947-6539

IS - 4

ER -