Light-sheet microscopy as a tool to understanding the behaviour of Polyion complex micelles for drug delivery

Fan Chen, Kecheng Li, Gene Hart-Smith, You Dan Xu, Yanyan Jiang, Hongxu Lu, Sandra Fok, Alexander Macmillian, Elvis Pandzic, Martina Stenzel*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Polyelectrolyte-protein complexes are widely used to deliver therapeutic proteins. Here, we present a method for imaging the release of drugs from polyion complex (PIC) micelles in 3D tumour spheroids using light-sheet microscopy. A negatively charged block copolymer was condensed with a positively charged model drug, hen egg white lysozyme (HEWL) by electrostatic interaction. We were able to observe the distribution of polymer and protein within the entire tumour spheroid, showing that the protein was released from the polyelectrolyte complex upon cell internalization at the peripheral cell layer of the spheroid.

Original languageEnglish
Pages (from-to)12618-12621
Number of pages4
JournalChemical Communications
Volume54
Issue number89
DOIs
Publication statusPublished - 18 Nov 2018
Externally publishedYes

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