Light-sheet microscopy as a tool to understanding the behaviour of Polyion complex micelles for drug delivery

Fan Chen; Kecheng Li; Gene Hart-Smith; You Dan Xu; Yanyan Jiang; Hongxu Lu; Sandra Fok; Alexander Macmillian; Elvis Pandzic; Martina Stenzel

doi:10.1039/C8CC04986F

Light-sheet microscopy as a tool to understanding the behaviour of Polyion complex micelles for drug delivery

Fan Chen, Kecheng Li, Gene Hart-Smith, You Dan Xu, Yanyan Jiang, Hongxu Lu, Sandra Fok, Alexander Macmillian, Elvis Pandzic, Martina Stenzel^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Polyelectrolyte-protein complexes are widely used to deliver therapeutic proteins. Here, we present a method for imaging the release of drugs from polyion complex (PIC) micelles in 3D tumour spheroids using light-sheet microscopy. A negatively charged block copolymer was condensed with a positively charged model drug, hen egg white lysozyme (HEWL) by electrostatic interaction. We were able to observe the distribution of polymer and protein within the entire tumour spheroid, showing that the protein was released from the polyelectrolyte complex upon cell internalization at the peripheral cell layer of the spheroid.

Original language	English
Pages (from-to)	12618-12621
Number of pages	4
Journal	Chemical Communications
Volume	54
Issue number	89
DOIs	https://doi.org/10.1039/C8CC04986F
Publication status	Published - 18 Nov 2018
Externally published	Yes

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abstract = "Polyelectrolyte-protein complexes are widely used to deliver therapeutic proteins. Here, we present a method for imaging the release of drugs from polyion complex (PIC) micelles in 3D tumour spheroids using light-sheet microscopy. A negatively charged block copolymer was condensed with a positively charged model drug, hen egg white lysozyme (HEWL) by electrostatic interaction. We were able to observe the distribution of polymer and protein within the entire tumour spheroid, showing that the protein was released from the polyelectrolyte complex upon cell internalization at the peripheral cell layer of the spheroid.",

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AU - Chen, Fan

AU - Li, Kecheng

AU - Hart-Smith, Gene

AU - Xu, You Dan

AU - Jiang, Yanyan

AU - Lu, Hongxu

AU - Fok, Sandra

AU - Macmillian, Alexander

AU - Pandzic, Elvis

AU - Stenzel, Martina

PY - 2018/11/18

Y1 - 2018/11/18

N2 - Polyelectrolyte-protein complexes are widely used to deliver therapeutic proteins. Here, we present a method for imaging the release of drugs from polyion complex (PIC) micelles in 3D tumour spheroids using light-sheet microscopy. A negatively charged block copolymer was condensed with a positively charged model drug, hen egg white lysozyme (HEWL) by electrostatic interaction. We were able to observe the distribution of polymer and protein within the entire tumour spheroid, showing that the protein was released from the polyelectrolyte complex upon cell internalization at the peripheral cell layer of the spheroid.

AB - Polyelectrolyte-protein complexes are widely used to deliver therapeutic proteins. Here, we present a method for imaging the release of drugs from polyion complex (PIC) micelles in 3D tumour spheroids using light-sheet microscopy. A negatively charged block copolymer was condensed with a positively charged model drug, hen egg white lysozyme (HEWL) by electrostatic interaction. We were able to observe the distribution of polymer and protein within the entire tumour spheroid, showing that the protein was released from the polyelectrolyte complex upon cell internalization at the peripheral cell layer of the spheroid.

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M3 - Article

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AN - SCOPUS:85056114903

SN - 1359-7345

VL - 54

SP - 12618

EP - 12621

JO - Chemical Communications

JF - Chemical Communications

IS - 89

ER -

Light-sheet microscopy as a tool to understanding the behaviour of Polyion complex micelles for drug delivery

Abstract

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