Light-sheet microscopy as a tool to understanding the behaviour of Polyion complex micelles for drug delivery

Fan Chen; Kecheng Li; Gene Hart-Smith; You Dan Xu; Yanyan Jiang; Hongxu Lu; Sandra Fok; Alexander Macmillian; Elvis Pandzic; Martina Stenzel

doi:10.1039/C8CC04986F

Light-sheet microscopy as a tool to understanding the behaviour of Polyion complex micelles for drug delivery

Fan Chen, Kecheng Li, Gene Hart-Smith, You Dan Xu, Yanyan Jiang, Hongxu Lu, Sandra Fok, Alexander Macmillian, Elvis Pandzic, Martina Stenzel^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Polyelectrolyte-protein complexes are widely used to deliver therapeutic proteins. Here, we present a method for imaging the release of drugs from polyion complex (PIC) micelles in 3D tumour spheroids using light-sheet microscopy. A negatively charged block copolymer was condensed with a positively charged model drug, hen egg white lysozyme (HEWL) by electrostatic interaction. We were able to observe the distribution of polymer and protein within the entire tumour spheroid, showing that the protein was released from the polyelectrolyte complex upon cell internalization at the peripheral cell layer of the spheroid.

Original language	English
Pages (from-to)	12618-12621
Number of pages	4
Journal	Chemical Communications
Volume	54
Issue number	89
DOIs	https://doi.org/10.1039/C8CC04986F
Publication status	Published - 18 Nov 2018
Externally published	Yes

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10.1039/C8CC04986F

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title = "Light-sheet microscopy as a tool to understanding the behaviour of Polyion complex micelles for drug delivery",

abstract = "Polyelectrolyte-protein complexes are widely used to deliver therapeutic proteins. Here, we present a method for imaging the release of drugs from polyion complex (PIC) micelles in 3D tumour spheroids using light-sheet microscopy. A negatively charged block copolymer was condensed with a positively charged model drug, hen egg white lysozyme (HEWL) by electrostatic interaction. We were able to observe the distribution of polymer and protein within the entire tumour spheroid, showing that the protein was released from the polyelectrolyte complex upon cell internalization at the peripheral cell layer of the spheroid.",

author = "Fan Chen and Kecheng Li and Gene Hart-Smith and Xu, {You Dan} and Yanyan Jiang and Hongxu Lu and Sandra Fok and Alexander Macmillian and Elvis Pandzic and Martina Stenzel",

year = "2018",

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day = "18",

doi = "10.1039/C8CC04986F",

language = "English",

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journal = "Chemical Communications",

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publisher = "Royal Society of Chemistry",

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Light-sheet microscopy as a tool to understanding the behaviour of Polyion complex micelles for drug delivery. / Chen, Fan; Li, Kecheng; Hart-Smith, Gene; Xu, You Dan; Jiang, Yanyan; Lu, Hongxu; Fok, Sandra; Macmillian, Alexander; Pandzic, Elvis; Stenzel, Martina.

In: Chemical Communications, Vol. 54, No. 89, 18.11.2018, p. 12618-12621.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Light-sheet microscopy as a tool to understanding the behaviour of Polyion complex micelles for drug delivery

AU - Chen, Fan

AU - Li, Kecheng

AU - Hart-Smith, Gene

AU - Xu, You Dan

AU - Jiang, Yanyan

AU - Lu, Hongxu

AU - Fok, Sandra

AU - Macmillian, Alexander

AU - Pandzic, Elvis

AU - Stenzel, Martina

PY - 2018/11/18

Y1 - 2018/11/18

N2 - Polyelectrolyte-protein complexes are widely used to deliver therapeutic proteins. Here, we present a method for imaging the release of drugs from polyion complex (PIC) micelles in 3D tumour spheroids using light-sheet microscopy. A negatively charged block copolymer was condensed with a positively charged model drug, hen egg white lysozyme (HEWL) by electrostatic interaction. We were able to observe the distribution of polymer and protein within the entire tumour spheroid, showing that the protein was released from the polyelectrolyte complex upon cell internalization at the peripheral cell layer of the spheroid.

AB - Polyelectrolyte-protein complexes are widely used to deliver therapeutic proteins. Here, we present a method for imaging the release of drugs from polyion complex (PIC) micelles in 3D tumour spheroids using light-sheet microscopy. A negatively charged block copolymer was condensed with a positively charged model drug, hen egg white lysozyme (HEWL) by electrostatic interaction. We were able to observe the distribution of polymer and protein within the entire tumour spheroid, showing that the protein was released from the polyelectrolyte complex upon cell internalization at the peripheral cell layer of the spheroid.

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DO - 10.1039/C8CC04986F

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VL - 54

SP - 12618

EP - 12621

JO - Chemical Communications

JF - Chemical Communications

SN - 1359-7345

IS - 89

ER -

Light-sheet microscopy as a tool to understanding the behaviour of Polyion complex micelles for drug delivery

Abstract

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