Linear dichroism of visible-region chromophores using M13 bacteriophage as an alignment scaffold

Matthew Tridgett*, Charles Moore-Kelly, Jean-Louis H. A. Duprey, Lorea Orueta Iturbe, Chi W. Tsang, Haydn A. Little, Sandeep K. Sandhu, Matthew R. Hicks, Timothy R. Dafforn, Alison Rodger

*Corresponding author for this work

Research output: Contribution to journalArticle

2 Citations (Scopus)
18 Downloads (Pure)

Abstract

It is a challenge within the field of biomimetics to recreate the properties of light-harvesting antennae found in plants and photosynthetic bacteria. Attempts to recreate these biological structures typically rely on the alignment of fluorescent moieties via attachment to an inert linear scaffold, e.g. DNA, RNA or amyloid fibrils, to enable Förster resonance energy transfer (FRET) between attached chromophores. While there has been some success in this approach, refinement of the alignment of the chromophores is often limited, which may limit the efficiency of energy transfer achieved. Here we demonstrate how linear dichroism spectroscopy may be used to ascertain the overall alignment of chromophores bound to the M13 bacteriophage, a model linear scaffold, and demonstrate how this may be used to distinguish between lack of FRET efficiency due to chromophore separation, and chromophore misalignment. This approach will allow the refinement of artificial light-harvesting antennae in a directed fashion.

Original languageEnglish
Pages (from-to)29535-29543
Number of pages9
JournalRSC Advances
Volume8
Issue number52
DOIs
Publication statusPublished - 2018

Bibliographical note

Copyright the Publisher 2018. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

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