Linking stomatal traits and expression of slow anion channel genes HvSLAH1 and HvSLAC1 with grain yield for increasing salinity tolerance in barley

Xiaohui Liu, Michelle Mak, Mohammad Babla, Feifei Wang, Guang Chen, Filip Veljanoski, Gang Wang, Sergey Shabala, Meixue Zhou, Zhong Hua Chen*

*Corresponding author for this work

Research output: Contribution to journalArticle

22 Citations (Scopus)
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Abstract

Soil salinity is an environmental and agricultural problem in many parts of the world. One of the keys to breeding barley for adaptation to salinity lies in a better understanding of the genetic control of stomatal regulation. We have employed a range of physiological (stomata assay, gas exchange, phylogenetic analysis, QTL analysis), and molecular techniques (RT-PCR and qPCR) to investigate stomatal behavior and genotypic variation in barley cultivars and a genetic population in four experimental trials. A set of relatively efficient and reliable methods were developed for the characterization of stomatal behavior of a large number of varieties and genetic lines. Furthermore, we found a large genetic variation of gas exchange and stomatal traits in barley in response to salinity stress. Salt-tolerant cultivar CM72 showed significantly larger stomatal aperture under 200 mM NaCl treatment than that of salt-sensitive cultivar Gairdner. Stomatal traits such as aperture width/length were found to significantly correlate with grain yield under salt treatment. Phenotypic characterization and QTL analysis of a segregating double haploid population of the CM72/Gairdner resulted in the identification of significant stomatal traits-related QTLs for salt tolerance. Moreover, expression analysis of the slow anion channel genes HvSLAH1 and HvSLAC1 demonstrated that their up-regulation is linked to higher barley grain yield in the field.

Original languageEnglish
Article number634
Pages (from-to)1-12
Number of pages12
JournalFrontiers in Plant Science
Volume5
Issue numberNOV
DOIs
Publication statusPublished - 25 Nov 2014

Bibliographical note

Copyright the Author(s) 2014. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

  • Gas exchange
  • Hordeum vulgare L
  • HvSLAC1
  • HvSLAH1
  • Quantitative trait loci
  • Soil salinity
  • Stomata

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