Linking transcriptomics and proteomics in cryopreserved human sperm

Maryam Hezavehei; Mehdi Mirzaei; Ghasem Hosseini Salekdeh; Abdolhossein Shahverdi; Poopak Eftekhari-Yazdi; Mohsen Sharafi

doi:10.1016/j.cryobiol.2017.10.083

Abstract

Cryopreservation induces negative impacts on important macromolecules such as proteins and transcriptome sperm that are transferred to the oocyte during fertilization. The use of combining novel genetic and proteomic techniques may hold the key to diagnosing and discovering specific biomarkers in pathogenic mechanisms involved in sperm cryopreservation. We investigated protein characteristics and transcript profile between freeze-thawed and fresh sperm from normozoospermic donors by Tandem Mass Tag (TMT) peptide labeling coupled with LC-MS/MS and RNA-seq respectively. Also, mouse oocyte activating capacity compared between cryopreserved sperm and fresh. In comparison with the fresh spermatozoa, there was a significant decrease in the Motility, progressive motility and increase in Caspase 3 activity rate in the cryopreserved spermatozoa.(p<0.01) We detected 2,912 proteins in human sperm. The main down-regulated proteins in cryopreserved sperm were the fertility and gluconeogenesis pathways proteins. Also key transcripts such as PRM1, PRM2, ATP8A1, were reduced after cryopreservation. We did not show significant differences in the oocyte activating capacity between cryopreserved sperm and fresh .(P>0.05).

Source of Funding: Royan institute

Conflict of Interest: None to disclose

Original language	English
Article number	S75
Pages (from-to)	175
Number of pages	1
Journal	Cryobiology
Volume	80
DOIs	https://doi.org/10.1016/j.cryobiol.2017.10.083
Publication status	Published - Feb 2018
Event	CRYO2017 - Hefei, China Duration: 21 Jul 2017 → 23 Jul 2017

Access to Document

10.1016/j.cryobiol.2017.10.083

Cite this

@article{769cec1ceab842db9f6326adb8b760a4,

title = "Linking transcriptomics and proteomics in cryopreserved human sperm",

abstract = "Cryopreservation induces negative impacts on important macromolecules such as proteins and transcriptome sperm that are transferred to the oocyte during fertilization. The use of combining novel genetic and proteomic techniques may hold the key to diagnosing and discovering specific biomarkers in pathogenic mechanisms involved in sperm cryopreservation. We investigated protein characteristics and transcript profile between freeze-thawed and fresh sperm from normozoospermic donors by Tandem Mass Tag (TMT) peptide labeling coupled with LC-MS/MS and RNA-seq respectively. Also, mouse oocyte activating capacity compared between cryopreserved sperm and fresh. In comparison with the fresh spermatozoa, there was a significant decrease in the Motility, progressive motility and increase in Caspase 3 activity rate in the cryopreserved spermatozoa.(p<0.01) We detected 2,912 proteins in human sperm. The main down-regulated proteins in cryopreserved sperm were the fertility and gluconeogenesis pathways proteins. Also key transcripts such as PRM1, PRM2, ATP8A1, were reduced after cryopreservation. We did not show significant differences in the oocyte activating capacity between cryopreserved sperm and fresh .(P>0.05).Source of Funding: Royan instituteConflict of Interest: None to disclose",

author = "Maryam Hezavehei and Mehdi Mirzaei and Salekdeh, {Ghasem Hosseini} and Abdolhossein Shahverdi and Poopak Eftekhari-Yazdi and Mohsen Sharafi",

year = "2018",

month = feb,

doi = "10.1016/j.cryobiol.2017.10.083",

language = "English",

volume = "80",

pages = "175",

journal = "Cryobiology",

issn = "0011-2240",

publisher = "Academic Press Inc.",

note = "CRYO2017 ; Conference date: 21-07-2017 Through 23-07-2017",

}

TY - JOUR

T1 - Linking transcriptomics and proteomics in cryopreserved human sperm

AU - Hezavehei, Maryam

AU - Mirzaei, Mehdi

AU - Salekdeh, Ghasem Hosseini

AU - Shahverdi, Abdolhossein

AU - Eftekhari-Yazdi, Poopak

AU - Sharafi, Mohsen

PY - 2018/2

Y1 - 2018/2

N2 - Cryopreservation induces negative impacts on important macromolecules such as proteins and transcriptome sperm that are transferred to the oocyte during fertilization. The use of combining novel genetic and proteomic techniques may hold the key to diagnosing and discovering specific biomarkers in pathogenic mechanisms involved in sperm cryopreservation. We investigated protein characteristics and transcript profile between freeze-thawed and fresh sperm from normozoospermic donors by Tandem Mass Tag (TMT) peptide labeling coupled with LC-MS/MS and RNA-seq respectively. Also, mouse oocyte activating capacity compared between cryopreserved sperm and fresh. In comparison with the fresh spermatozoa, there was a significant decrease in the Motility, progressive motility and increase in Caspase 3 activity rate in the cryopreserved spermatozoa.(p<0.01) We detected 2,912 proteins in human sperm. The main down-regulated proteins in cryopreserved sperm were the fertility and gluconeogenesis pathways proteins. Also key transcripts such as PRM1, PRM2, ATP8A1, were reduced after cryopreservation. We did not show significant differences in the oocyte activating capacity between cryopreserved sperm and fresh .(P>0.05).Source of Funding: Royan instituteConflict of Interest: None to disclose

AB - Cryopreservation induces negative impacts on important macromolecules such as proteins and transcriptome sperm that are transferred to the oocyte during fertilization. The use of combining novel genetic and proteomic techniques may hold the key to diagnosing and discovering specific biomarkers in pathogenic mechanisms involved in sperm cryopreservation. We investigated protein characteristics and transcript profile between freeze-thawed and fresh sperm from normozoospermic donors by Tandem Mass Tag (TMT) peptide labeling coupled with LC-MS/MS and RNA-seq respectively. Also, mouse oocyte activating capacity compared between cryopreserved sperm and fresh. In comparison with the fresh spermatozoa, there was a significant decrease in the Motility, progressive motility and increase in Caspase 3 activity rate in the cryopreserved spermatozoa.(p<0.01) We detected 2,912 proteins in human sperm. The main down-regulated proteins in cryopreserved sperm were the fertility and gluconeogenesis pathways proteins. Also key transcripts such as PRM1, PRM2, ATP8A1, were reduced after cryopreservation. We did not show significant differences in the oocyte activating capacity between cryopreserved sperm and fresh .(P>0.05).Source of Funding: Royan instituteConflict of Interest: None to disclose

U2 - 10.1016/j.cryobiol.2017.10.083

DO - 10.1016/j.cryobiol.2017.10.083

M3 - Meeting abstract

SN - 0011-2240

VL - 80

SP - 175

JO - Cryobiology

JF - Cryobiology

M1 - S75

T2 - CRYO2017

Y2 - 21 July 2017 through 23 July 2017

ER -

Linking transcriptomics and proteomics in cryopreserved human sperm

Abstract

Access to Document

Fingerprint

Cite this