Linking transcriptomics and proteomics in cryopreserved human sperm

Maryam Hezavehei, Mehdi Mirzaei, Ghasem Hosseini Salekdeh, Abdolhossein Shahverdi, Poopak Eftekhari-Yazdi, Mohsen Sharafi

Research output: Contribution to journalMeeting abstract


Cryopreservation induces negative impacts on important macromolecules such as proteins and transcriptome sperm that are transferred to the oocyte during fertilization. The use of combining novel genetic and proteomic techniques may hold the key to diagnosing and discovering specific biomarkers in pathogenic mechanisms involved in sperm cryopreservation. We investigated protein characteristics and transcript profile between freeze-thawed and fresh sperm from normozoospermic donors by Tandem Mass Tag (TMT) peptide labeling coupled with LC-MS/MS and RNA-seq respectively. Also, mouse oocyte activating capacity compared between cryopreserved sperm and fresh. In comparison with the fresh spermatozoa, there was a significant decrease in the Motility, progressive motility and increase in Caspase 3 activity rate in the cryopreserved spermatozoa.(p<0.01) We detected 2,912 proteins in human sperm. The main down-regulated proteins in cryopreserved sperm were the fertility and gluconeogenesis pathways proteins. Also key transcripts such as PRM1, PRM2, ATP8A1, were reduced after cryopreservation. We did not show significant differences in the oocyte activating capacity between cryopreserved sperm and fresh .(P>0.05).

Source of Funding: Royan institute

Conflict of Interest: None to disclose
Original languageEnglish
Article numberS75
Pages (from-to)175
Number of pages1
Publication statusPublished - Feb 2018
EventCRYO2017 - Hefei, China
Duration: 21 Jul 201723 Jul 2017


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