Liquid crystal as sensing platforms for determining the effect of graphene oxide-based materials on phospholipid membranes and monitoring antibacterial activity

Yibin Wei, Chang Hyun Jang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Understanding the mechanism of the cellular interaction of graphene oxide (GO)-based materials is vital for their biomedical and environmental applications. In this study, we developed a promising method for imaging the interaction between chitosan (CS)-reinforced GO and the phospholipid membrane based on a nematic liquid crystal (LC) sensing platform. Direct observation of the CS-GO-induced rupture of the pre-adsorbed phospholipid monolayer supported by an LC thin film was achieved using a polarizing optical microscope (POM). The disruption process was clearly monitored in real-time using this method. Moreover, because lipids at the Gram-negative bacterial cell membrane could be transferred onto the interfaces of micro-meter-sized sessile LC droplets, we found the LC droplets could be used to distinguish live Escherichia coli (E. coli) cells from dead cells. CS-GO efficiently reduced the cellular integrity of E. coli which was detectable by LC droplets; thus, the LC-based approach could be used for monitoring the antibacterial activities of GO-related materials. These experimental LC sensing platforms offer simple and direct observation methods for evaluating the effect of GO-based materials on the cell membrane and may be useful in corresponding antibacterial applications.

Original languageEnglish
Pages (from-to)72-80
Number of pages9
JournalSensors and Actuators, B: Chemical
Volume254
DOIs
Publication statusPublished - Jan 2018
Externally publishedYes

Keywords

  • 4-Cyano-4′-pentylbiphenyl
  • antibacterial
  • chitosan
  • graphene oxide
  • liquid crystal
  • phospholipids

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