Localisation and distribution of the major allergens in apple fruits

Gorji Marzban; Helene Puehringer; Rickmer Dey; Sabine Brynda; Yan Ma; Alessio Martinelli; Marzio Zaccarini; Erik Van Der Weg; Zora Housley; Daniel Kolarich; Friedrich Altmann; Margit Laimer

doi:10.1016/j.plantsci.2005.03.027

Localisation and distribution of the major allergens in apple fruits

Gorji Marzban, Helene Puehringer, Rickmer Dey, Sabine Brynda, Yan Ma, Alessio Martinelli, Marzio Zaccarini, Erik Van Der Weg, Zora Housley, Daniel Kolarich, Friedrich Altmann, Margit Laimer^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

118 Citations (Scopus)

Abstract

The importance of apple allergens, in particular Mal d 1, a Bet v 1 homologue for the pollen-fruit syndrome in Northern Europe, and Mal d 3, responsible for true fruit allergy in Southern Europe, has been repeatedly emphasized. However, little is known about the distribution pattern of major allergens in fruits and whether differences exist among different cultivars. Transcript expression of Mal d 1 isoforms and Mal d 3 was examined by RealTime-PCR and Northern analysis, respectively. An immuno-tissue-print (ITP) assay was developed to localise major allergens in apple fruit tissue and a Mal d 1 specific, patient independent ELISA was established. ITP analyses show that Mal d 1 and Mal d 2 are distributed throughout the apple pulp and peel, while Mal d 3 is restricted to the peel. Data obtained by ELISA reveal a variation of Mal d 1 content ranging from 0.84 to 33.17 μg/g fresh weight in 39 selected cultivars. Different apple cultivars show a markedly different expression of major allergens; this finding will influence the development of diagnostic tools as well as the dietary management of allergic individuals.

Original language	English
Pages (from-to)	387-394
Number of pages	8
Journal	Plant Science
Volume	169
Issue number	2
DOIs	https://doi.org/10.1016/j.plantsci.2005.03.027
Publication status	Published - Aug 2005

Keywords

Cultivar screening
ELISA
Immuno-tissue-prints
Mal d 1 isoforms
Mal d 3

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title = "Localisation and distribution of the major allergens in apple fruits",

abstract = "The importance of apple allergens, in particular Mal d 1, a Bet v 1 homologue for the pollen-fruit syndrome in Northern Europe, and Mal d 3, responsible for true fruit allergy in Southern Europe, has been repeatedly emphasized. However, little is known about the distribution pattern of major allergens in fruits and whether differences exist among different cultivars. Transcript expression of Mal d 1 isoforms and Mal d 3 was examined by RealTime-PCR and Northern analysis, respectively. An immuno-tissue-print (ITP) assay was developed to localise major allergens in apple fruit tissue and a Mal d 1 specific, patient independent ELISA was established. ITP analyses show that Mal d 1 and Mal d 2 are distributed throughout the apple pulp and peel, while Mal d 3 is restricted to the peel. Data obtained by ELISA reveal a variation of Mal d 1 content ranging from 0.84 to 33.17 μg/g fresh weight in 39 selected cultivars. Different apple cultivars show a markedly different expression of major allergens; this finding will influence the development of diagnostic tools as well as the dietary management of allergic individuals.",

keywords = "Cultivar screening, ELISA, Immuno-tissue-prints, Mal d 1 isoforms, Mal d 3",

author = "Gorji Marzban and Helene Puehringer and Rickmer Dey and Sabine Brynda and Yan Ma and Alessio Martinelli and Marzio Zaccarini and {Van Der Weg}, Erik and Zora Housley and Daniel Kolarich and Friedrich Altmann and Margit Laimer",

year = "2005",

month = aug,

doi = "10.1016/j.plantsci.2005.03.027",

language = "English",

volume = "169",

pages = "387--394",

journal = "Plant Science",

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AU - Marzban, Gorji

AU - Puehringer, Helene

AU - Dey, Rickmer

AU - Brynda, Sabine

AU - Ma, Yan

AU - Martinelli, Alessio

AU - Zaccarini, Marzio

AU - Van Der Weg, Erik

AU - Housley, Zora

AU - Kolarich, Daniel

AU - Altmann, Friedrich

AU - Laimer, Margit

PY - 2005/8

Y1 - 2005/8

N2 - The importance of apple allergens, in particular Mal d 1, a Bet v 1 homologue for the pollen-fruit syndrome in Northern Europe, and Mal d 3, responsible for true fruit allergy in Southern Europe, has been repeatedly emphasized. However, little is known about the distribution pattern of major allergens in fruits and whether differences exist among different cultivars. Transcript expression of Mal d 1 isoforms and Mal d 3 was examined by RealTime-PCR and Northern analysis, respectively. An immuno-tissue-print (ITP) assay was developed to localise major allergens in apple fruit tissue and a Mal d 1 specific, patient independent ELISA was established. ITP analyses show that Mal d 1 and Mal d 2 are distributed throughout the apple pulp and peel, while Mal d 3 is restricted to the peel. Data obtained by ELISA reveal a variation of Mal d 1 content ranging from 0.84 to 33.17 μg/g fresh weight in 39 selected cultivars. Different apple cultivars show a markedly different expression of major allergens; this finding will influence the development of diagnostic tools as well as the dietary management of allergic individuals.

AB - The importance of apple allergens, in particular Mal d 1, a Bet v 1 homologue for the pollen-fruit syndrome in Northern Europe, and Mal d 3, responsible for true fruit allergy in Southern Europe, has been repeatedly emphasized. However, little is known about the distribution pattern of major allergens in fruits and whether differences exist among different cultivars. Transcript expression of Mal d 1 isoforms and Mal d 3 was examined by RealTime-PCR and Northern analysis, respectively. An immuno-tissue-print (ITP) assay was developed to localise major allergens in apple fruit tissue and a Mal d 1 specific, patient independent ELISA was established. ITP analyses show that Mal d 1 and Mal d 2 are distributed throughout the apple pulp and peel, while Mal d 3 is restricted to the peel. Data obtained by ELISA reveal a variation of Mal d 1 content ranging from 0.84 to 33.17 μg/g fresh weight in 39 selected cultivars. Different apple cultivars show a markedly different expression of major allergens; this finding will influence the development of diagnostic tools as well as the dietary management of allergic individuals.

KW - Cultivar screening

KW - ELISA

KW - Immuno-tissue-prints

KW - Mal d 1 isoforms

KW - Mal d 3

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DO - 10.1016/j.plantsci.2005.03.027

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SN - 0168-9452

VL - 169

SP - 387

EP - 394

JO - Plant Science

JF - Plant Science

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ER -

Localisation and distribution of the major allergens in apple fruits

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Keywords

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