Longitudinal measurements of syrinx size in a rat model of posttraumatic syringomyelia

Elmira Najafi, Lynne E. Bilston, Xin Song, Andre Bongers, Marcus A. Stoodley, Shaokoon Cheng, Sarah J. Hemley

Research output: Contribution to journalArticleResearchpeer-review

Abstract

OBJECTIVE: Syringomyelia pathophysiology is commonly studied using rodent models. However, in vivo studies of posttraumatic syringomyelia have been limited by the size of animals and lack of reliable noninvasive evaluation techniques. Imaging the rat spinal cord is particularly challenging because the spinal cord diameter is approximately 1-3 mm, and pathological lesions within the spinal cord parenchyma are even smaller. The standard technique has been histological evaluation, but this has its limitations. The aim of the present study was to determine whether syrinx size could be reliably measured using a preclinical high-field MRI animal system in a rat model of posttraumatic syringomyelia.

METHODS: The authors used an existing rat model of posttraumatic syringomyelia, which was created using a controlled pneumatic compression device to produce the initial spinal cord injury, followed by a subarachnoid injection of kaolin to produce arachnoiditis. T2-weighted MRI was performed on each animal using a 9.4-T scanner at 7, 10, and 13 weeks after injury. Animals were killed and syrinx sizes were calculated from in vivo MRI and histological studies.

RESULTS: MRI measurements of syrinx volume and length were closely correlated to histological measurements across all time points (Pearson product moment correlation coefficient r = +/- 0.93 and 0.79, respectively).

CONCLUSIONS: This study demonstrates that high-field T2-weighted MRI can be used to measure syrinx size, and data correlate well with syrinx size measured using histological methods. Preclinical MRI may be a valuable noninvasive technique for tracking syrinx formation and enlargement in animal models of syringomyelia.

LanguageEnglish
Pages941-948
Number of pages8
JournalJournal of Neurosurgery: Spine
Volume24
Issue number6
DOIs
Publication statusPublished - 1 Jun 2016

Keywords

  • ADC = apparent diffusion coefficient
  • MRI
  • posttraumatic syringomyelia
  • RARE = rapid acquisition with relaxation enhancement
  • spinal cord injury
  • syrinx

Cite this

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title = "Longitudinal measurements of syrinx size in a rat model of posttraumatic syringomyelia",
abstract = "OBJECTIVE: Syringomyelia pathophysiology is commonly studied using rodent models. However, in vivo studies of posttraumatic syringomyelia have been limited by the size of animals and lack of reliable noninvasive evaluation techniques. Imaging the rat spinal cord is particularly challenging because the spinal cord diameter is approximately 1-3 mm, and pathological lesions within the spinal cord parenchyma are even smaller. The standard technique has been histological evaluation, but this has its limitations. The aim of the present study was to determine whether syrinx size could be reliably measured using a preclinical high-field MRI animal system in a rat model of posttraumatic syringomyelia.METHODS: The authors used an existing rat model of posttraumatic syringomyelia, which was created using a controlled pneumatic compression device to produce the initial spinal cord injury, followed by a subarachnoid injection of kaolin to produce arachnoiditis. T2-weighted MRI was performed on each animal using a 9.4-T scanner at 7, 10, and 13 weeks after injury. Animals were killed and syrinx sizes were calculated from in vivo MRI and histological studies.RESULTS: MRI measurements of syrinx volume and length were closely correlated to histological measurements across all time points (Pearson product moment correlation coefficient r = +/- 0.93 and 0.79, respectively).CONCLUSIONS: This study demonstrates that high-field T2-weighted MRI can be used to measure syrinx size, and data correlate well with syrinx size measured using histological methods. Preclinical MRI may be a valuable noninvasive technique for tracking syrinx formation and enlargement in animal models of syringomyelia.",
keywords = "ADC = apparent diffusion coefficient, MRI, posttraumatic syringomyelia, RARE = rapid acquisition with relaxation enhancement, spinal cord injury, syrinx",
author = "Elmira Najafi and Bilston, {Lynne E.} and Xin Song and Andre Bongers and Stoodley, {Marcus A.} and Shaokoon Cheng and Hemley, {Sarah J.}",
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Longitudinal measurements of syrinx size in a rat model of posttraumatic syringomyelia. / Najafi, Elmira; Bilston, Lynne E.; Song, Xin; Bongers, Andre; Stoodley, Marcus A.; Cheng, Shaokoon; Hemley, Sarah J.

In: Journal of Neurosurgery: Spine, Vol. 24, No. 6, 01.06.2016, p. 941-948.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Longitudinal measurements of syrinx size in a rat model of posttraumatic syringomyelia

AU - Najafi, Elmira

AU - Bilston, Lynne E.

AU - Song, Xin

AU - Bongers, Andre

AU - Stoodley, Marcus A.

AU - Cheng, Shaokoon

AU - Hemley, Sarah J.

PY - 2016/6/1

Y1 - 2016/6/1

N2 - OBJECTIVE: Syringomyelia pathophysiology is commonly studied using rodent models. However, in vivo studies of posttraumatic syringomyelia have been limited by the size of animals and lack of reliable noninvasive evaluation techniques. Imaging the rat spinal cord is particularly challenging because the spinal cord diameter is approximately 1-3 mm, and pathological lesions within the spinal cord parenchyma are even smaller. The standard technique has been histological evaluation, but this has its limitations. The aim of the present study was to determine whether syrinx size could be reliably measured using a preclinical high-field MRI animal system in a rat model of posttraumatic syringomyelia.METHODS: The authors used an existing rat model of posttraumatic syringomyelia, which was created using a controlled pneumatic compression device to produce the initial spinal cord injury, followed by a subarachnoid injection of kaolin to produce arachnoiditis. T2-weighted MRI was performed on each animal using a 9.4-T scanner at 7, 10, and 13 weeks after injury. Animals were killed and syrinx sizes were calculated from in vivo MRI and histological studies.RESULTS: MRI measurements of syrinx volume and length were closely correlated to histological measurements across all time points (Pearson product moment correlation coefficient r = +/- 0.93 and 0.79, respectively).CONCLUSIONS: This study demonstrates that high-field T2-weighted MRI can be used to measure syrinx size, and data correlate well with syrinx size measured using histological methods. Preclinical MRI may be a valuable noninvasive technique for tracking syrinx formation and enlargement in animal models of syringomyelia.

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KW - ADC = apparent diffusion coefficient

KW - MRI

KW - posttraumatic syringomyelia

KW - RARE = rapid acquisition with relaxation enhancement

KW - spinal cord injury

KW - syrinx

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U2 - 10.3171/2015.10.SPINE15538

DO - 10.3171/2015.10.SPINE15538

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