LOSOM: phase relief imaging can be achieved with confocal system

Tong Peng; Hao Xie; Yichen Ding; Yiqing Lu; Dayong Jin; Peng Xi

doi:10.1117/12.2000809

LOSOM: phase relief imaging can be achieved with confocal system

Tong Peng, Hao Xie, Yichen Ding, Yiqing Lu, Dayong Jin, Peng Xi^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

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Abstract

We reported recently that laser oblique scanning optical microscopy (LOSOM) is able to obtain a relief image in transparent sample directly. To optimize the performance of LOSOM, the parameters such as numerical aperture, the distance between the specimen and the fluorescent medium and the pinhole size are investigated in this work. A beam blocker is introduced in light path which enhances dramatically the visualization of local phase difference.

Original language	English
Title of host publication	Optics in Health Care and Biomedical Optics V
Subtitle of host publication	5-7 November 2012, Beijing, China
Editors	Qingming Luo, Ying Gu, Xingde D. Li
Place of Publication	Bellingham, Washington
Publisher	SPIE
Pages	85531C-1-85531C-6
Number of pages	6
ISBN (Print)	9780819493088
DOIs	https://doi.org/10.1117/12.2000809
Publication status	Published - 2012
Event	Optics in Health Care and Biomedical Optics V - Beijing, China Duration: 5 Nov 2012 → 7 Nov 2012

Publication series

Name	Proceedings of SPIE
Volume	8553
ISSN (Print)	0277-786X

Name	Progress in biomedical optics and imaging
Number	42
Volume	13
ISSN (Print)	1605-7422

Other

Other	Optics in Health Care and Biomedical Optics V
Country/Territory	China
City	Beijing
Period	5/11/12 → 7/11/12

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10.1117/12.2000809

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Peng, T., Xie, H., Ding, Y., Lu, Y., Jin, D., & Xi, P. (2012). LOSOM: phase relief imaging can be achieved with confocal system. In Q. Luo, Y. Gu, & X. D. Li (Eds.), Optics in Health Care and Biomedical Optics V: 5-7 November 2012, Beijing, China (pp. 85531C-1-85531C-6). Article 85531C (Proceedings of SPIE; Vol. 8553), (Progress in biomedical optics and imaging; Vol. 13, No. 42). SPIE. https://doi.org/10.1117/12.2000809

Peng, Tong ; Xie, Hao ; Ding, Yichen et al. / LOSOM : phase relief imaging can be achieved with confocal system. Optics in Health Care and Biomedical Optics V: 5-7 November 2012, Beijing, China. editor / Qingming Luo ; Ying Gu ; Xingde D. Li. Bellingham, Washington : SPIE, 2012. pp. 85531C-1-85531C-6 (Proceedings of SPIE). (Progress in biomedical optics and imaging; 42).

@inproceedings{c96eac1b55be4b728ce10cd2e602f8e9,

title = "LOSOM: phase relief imaging can be achieved with confocal system",

abstract = "We reported recently that laser oblique scanning optical microscopy (LOSOM) is able to obtain a relief image in transparent sample directly. To optimize the performance of LOSOM, the parameters such as numerical aperture, the distance between the specimen and the fluorescent medium and the pinhole size are investigated in this work. A beam blocker is introduced in light path which enhances dramatically the visualization of local phase difference.",

author = "Tong Peng and Hao Xie and Yichen Ding and Yiqing Lu and Dayong Jin and Peng Xi",

year = "2012",

doi = "10.1117/12.2000809",

language = "English",

isbn = "9780819493088",

series = "Proceedings of SPIE",

publisher = "SPIE",

pages = "85531C--1--85531C--6",

editor = "Qingming Luo and Ying Gu and Li, {Xingde D.}",

booktitle = "Optics in Health Care and Biomedical Optics V",

address = "United States",

note = "Optics in Health Care and Biomedical Optics V ; Conference date: 05-11-2012 Through 07-11-2012",

}

Peng, T, Xie, H, Ding, Y, Lu, Y, Jin, D & Xi, P 2012, LOSOM: phase relief imaging can be achieved with confocal system. in Q Luo, Y Gu & XD Li (eds), Optics in Health Care and Biomedical Optics V: 5-7 November 2012, Beijing, China., 85531C, Proceedings of SPIE, vol. 8553, Progress in biomedical optics and imaging, no. 42, vol. 13, SPIE, Bellingham, Washington, pp. 85531C-1-85531C-6, Optics in Health Care and Biomedical Optics V, Beijing, China, 5/11/12. https://doi.org/10.1117/12.2000809

LOSOM: phase relief imaging can be achieved with confocal system. / Peng, Tong; Xie, Hao; Ding, Yichen et al.
Optics in Health Care and Biomedical Optics V: 5-7 November 2012, Beijing, China. ed. / Qingming Luo; Ying Gu; Xingde D. Li. Bellingham, Washington: SPIE, 2012. p. 85531C-1-85531C-6 85531C (Proceedings of SPIE; Vol. 8553), (Progress in biomedical optics and imaging; Vol. 13, No. 42).

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

TY - GEN

T1 - LOSOM

T2 - Optics in Health Care and Biomedical Optics V

AU - Peng, Tong

AU - Xie, Hao

AU - Ding, Yichen

AU - Lu, Yiqing

AU - Jin, Dayong

AU - Xi, Peng

PY - 2012

Y1 - 2012

N2 - We reported recently that laser oblique scanning optical microscopy (LOSOM) is able to obtain a relief image in transparent sample directly. To optimize the performance of LOSOM, the parameters such as numerical aperture, the distance between the specimen and the fluorescent medium and the pinhole size are investigated in this work. A beam blocker is introduced in light path which enhances dramatically the visualization of local phase difference.

AB - We reported recently that laser oblique scanning optical microscopy (LOSOM) is able to obtain a relief image in transparent sample directly. To optimize the performance of LOSOM, the parameters such as numerical aperture, the distance between the specimen and the fluorescent medium and the pinhole size are investigated in this work. A beam blocker is introduced in light path which enhances dramatically the visualization of local phase difference.

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U2 - 10.1117/12.2000809

DO - 10.1117/12.2000809

M3 - Conference proceeding contribution

AN - SCOPUS:84875941984

SN - 9780819493088

T3 - Proceedings of SPIE

SP - 85531C-1-85531C-6

BT - Optics in Health Care and Biomedical Optics V

A2 - Luo, Qingming

A2 - Gu, Ying

A2 - Li, Xingde D.

PB - SPIE

CY - Bellingham, Washington

Y2 - 5 November 2012 through 7 November 2012

ER -

Peng T, Xie H, Ding Y, Lu Y, Jin D, Xi P. LOSOM: phase relief imaging can be achieved with confocal system. In Luo Q, Gu Y, Li XD, editors, Optics in Health Care and Biomedical Optics V: 5-7 November 2012, Beijing, China. Bellingham, Washington: SPIE. 2012. p. 85531C-1-85531C-6. 85531C. (Proceedings of SPIE). (Progress in biomedical optics and imaging; 42). doi: 10.1117/12.2000809

LOSOM: phase relief imaging can be achieved with confocal system

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