LOSOM: phase relief imaging can be achieved with confocal system

Tong Peng, Hao Xie, Yichen Ding, Yiqing Lu, Dayong Jin, Peng Xi*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contributionpeer-review

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Abstract

We reported recently that laser oblique scanning optical microscopy (LOSOM) is able to obtain a relief image in transparent sample directly. To optimize the performance of LOSOM, the parameters such as numerical aperture, the distance between the specimen and the fluorescent medium and the pinhole size are investigated in this work. A beam blocker is introduced in light path which enhances dramatically the visualization of local phase difference.

Original languageEnglish
Title of host publicationOptics in Health Care and Biomedical Optics V
Subtitle of host publication5-7 November 2012, Beijing, China
EditorsQingming Luo, Ying Gu, Xingde D. Li
Place of PublicationBellingham, Washington
PublisherSPIE
Pages85531C-1-85531C-6
Number of pages6
ISBN (Print)9780819493088
DOIs
Publication statusPublished - 2012
EventOptics in Health Care and Biomedical Optics V - Beijing, China
Duration: 5 Nov 20127 Nov 2012

Publication series

NameProceedings of SPIE
Volume8553
ISSN (Print)0277-786X
NameProgress in biomedical optics and imaging
Number42
Volume13
ISSN (Print)1605-7422

Other

OtherOptics in Health Care and Biomedical Optics V
CountryChina
CityBeijing
Period5/11/127/11/12

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