Low molecular weight sulfated chitosan

neuroprotective effect on rotenone-induced in vitro Parkinson’s disease

Venkatesan Manigandan, Jagatheesan Nataraj, Ramachandran Karthik, Thamilarasan Manivasagam, Ramachandran Saravanan*, Arokyasamy Justin Thenmozhi, Musthafa Mohamed Essa, Gilles J. Guillemin

*Corresponding author for this work

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The present investigation was an attempt to study the effect of low molecular weight sulfated chitosan (LMWSC) on in vitro rotenone model of Parkinson’s disease (PD) by evaluating cell viability, oxidative stress, mitochondrial membrane potential, DNA fragmentation, and apoptosis. Incubation of SH-SY5Y cells with 100 nm rotenone resulted in neuronal cell death, redox imbalanced mitochondrial dysfunction, DNA fragmentation, condensation, and apoptotic cellular morphology. Rotenone exposure enhanced the expression of preapoptotic (cytochrome C (cyto c), caspase-3, -8, -9, and Bax) and down-regulated the expression of anti-apoptotic (Bcl-2) markers. Reduction of the intracellular reactive oxygen species (ROS) levels ensued due to pretreatment of LMWSC along with consequent normalization of antioxidant enzymes, mitigation of rotenone induced mitochondrial dysfunction and apoptosis. Our current findings suggested that LMWSC exhibit the pronounced neuroprotective effects, which could be due to its antioxidant, mitochondrial protection, and anti-apoptotic properties. We thus conclude that LMWSC could be developed as a novel therapeutic molecule for the benefit of reducing the consequences of PD. However, further extensive preclinical and clinical studies are warranted.

Original languageEnglish
Pages (from-to)505-515
Number of pages11
JournalNeurotoxicity Research
Volume35
Issue number3
DOIs
Publication statusPublished - Apr 2019

Keywords

  • Apoptosis
  • Mitochondrial dysfunction
  • Neuronal damage
  • Oxidative stress
  • Rotenone
  • Sulfated chitosan

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