Lsd1 ablation triggers metabolic reprogramming of brown adipose tissue

Delphine Duteil, Milica Tosic, Franziska Lausecker, Hatice Z. Nenseth, Judith M. Müller, Sylvia Urban, Dominica Willmann, Kerstin Petroll, Nadia Messaddeq, Laura Arrigoni, Thomas Manke, Jan Wilhelm Kornfeld, Jens C. Brüning, Vyacheslav Zagoriy, Michael Meret, Jörn Dengjel, Toufike Kanouni, Roland Schüle

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Previous work indicated that lysine-specific demethylase 1 (Lsd1) can positively regulate the oxidative and thermogenic capacities of white and beige adipocytes. Here we investigate the role of Lsd1 in brown adipose tissue (BAT) and find that BAT-selective Lsd1 ablation induces a shift from oxidative to glycolytic metabolism. This shift is associated with downregulation of BAT-specific and upregulation of white adipose tissue (WAT)-selective gene expression. This results in the accumulation of di- and triacylglycerides and culminates in a profound whitening of BAT in aged Lsd1-deficient mice. Further studies show that Lsd1 maintains BAT properties via a dual role. It activates BAT-selective gene expression in concert with the transcription factor Nrf1 and represses WAT-selective genes through recruitment of the CoREST complex. In conclusion, our data uncover Lsd1 as a key regulator of gene expression and metabolic function in BAT.

LanguageEnglish
Pages1008-1021
Number of pages14
JournalCell Reports
Volume17
Issue number4
DOIs
Publication statusPublished - 18 Oct 2016
Externally publishedYes

Fingerprint

Brown Adipose Tissue
Ablation
Lysine
Tissue
White Adipose Tissue
Gene expression
Gene Expression
White Adipocytes
Regulator Genes
Transcription Factors
Up-Regulation
Down-Regulation
Metabolism
Genes

Bibliographical note

Copyright the Author(s) 2016. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

  • adipocyte
  • brown adipose tissue
  • carbohydrate metabolism
  • CoREST
  • epigenetics
  • lipid metabolism
  • lysine-specific demethylase 1
  • obesity
  • thermogenesis
  • white adipose tissue

Cite this

Duteil, D., Tosic, M., Lausecker, F., Nenseth, H. Z., Müller, J. M., Urban, S., ... Schüle, R. (2016). Lsd1 ablation triggers metabolic reprogramming of brown adipose tissue. Cell Reports, 17(4), 1008-1021. https://doi.org/10.1016/j.celrep.2016.09.053
Duteil, Delphine ; Tosic, Milica ; Lausecker, Franziska ; Nenseth, Hatice Z. ; Müller, Judith M. ; Urban, Sylvia ; Willmann, Dominica ; Petroll, Kerstin ; Messaddeq, Nadia ; Arrigoni, Laura ; Manke, Thomas ; Kornfeld, Jan Wilhelm ; Brüning, Jens C. ; Zagoriy, Vyacheslav ; Meret, Michael ; Dengjel, Jörn ; Kanouni, Toufike ; Schüle, Roland. / Lsd1 ablation triggers metabolic reprogramming of brown adipose tissue. In: Cell Reports. 2016 ; Vol. 17, No. 4. pp. 1008-1021.
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Duteil, D, Tosic, M, Lausecker, F, Nenseth, HZ, Müller, JM, Urban, S, Willmann, D, Petroll, K, Messaddeq, N, Arrigoni, L, Manke, T, Kornfeld, JW, Brüning, JC, Zagoriy, V, Meret, M, Dengjel, J, Kanouni, T & Schüle, R 2016, 'Lsd1 ablation triggers metabolic reprogramming of brown adipose tissue', Cell Reports, vol. 17, no. 4, pp. 1008-1021. https://doi.org/10.1016/j.celrep.2016.09.053

Lsd1 ablation triggers metabolic reprogramming of brown adipose tissue. / Duteil, Delphine; Tosic, Milica; Lausecker, Franziska; Nenseth, Hatice Z.; Müller, Judith M.; Urban, Sylvia; Willmann, Dominica; Petroll, Kerstin; Messaddeq, Nadia; Arrigoni, Laura; Manke, Thomas; Kornfeld, Jan Wilhelm; Brüning, Jens C.; Zagoriy, Vyacheslav; Meret, Michael; Dengjel, Jörn; Kanouni, Toufike; Schüle, Roland.

In: Cell Reports, Vol. 17, No. 4, 18.10.2016, p. 1008-1021.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Duteil, Delphine

AU - Tosic, Milica

AU - Lausecker, Franziska

AU - Nenseth, Hatice Z.

AU - Müller, Judith M.

AU - Urban, Sylvia

AU - Willmann, Dominica

AU - Petroll, Kerstin

AU - Messaddeq, Nadia

AU - Arrigoni, Laura

AU - Manke, Thomas

AU - Kornfeld, Jan Wilhelm

AU - Brüning, Jens C.

AU - Zagoriy, Vyacheslav

AU - Meret, Michael

AU - Dengjel, Jörn

AU - Kanouni, Toufike

AU - Schüle, Roland

N1 - Copyright the Author(s) 2016. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

PY - 2016/10/18

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N2 - Previous work indicated that lysine-specific demethylase 1 (Lsd1) can positively regulate the oxidative and thermogenic capacities of white and beige adipocytes. Here we investigate the role of Lsd1 in brown adipose tissue (BAT) and find that BAT-selective Lsd1 ablation induces a shift from oxidative to glycolytic metabolism. This shift is associated with downregulation of BAT-specific and upregulation of white adipose tissue (WAT)-selective gene expression. This results in the accumulation of di- and triacylglycerides and culminates in a profound whitening of BAT in aged Lsd1-deficient mice. Further studies show that Lsd1 maintains BAT properties via a dual role. It activates BAT-selective gene expression in concert with the transcription factor Nrf1 and represses WAT-selective genes through recruitment of the CoREST complex. In conclusion, our data uncover Lsd1 as a key regulator of gene expression and metabolic function in BAT.

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Duteil D, Tosic M, Lausecker F, Nenseth HZ, Müller JM, Urban S et al. Lsd1 ablation triggers metabolic reprogramming of brown adipose tissue. Cell Reports. 2016 Oct 18;17(4):1008-1021. https://doi.org/10.1016/j.celrep.2016.09.053