Luminescent microspheres resolved from strong background on an automated time-gated luminescence microscopy workstation

Leonard G C Hamey, Russell E. Connally, Simon Wong Too Yen, Thomas S. Lawson, James A. Piper, Jon Iredell

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contribution

2 Citations (Scopus)
6 Downloads (Pure)

Abstract

Fluorescence microscopy is a powerful tool for the rapid identification of target organisms. However, natural autofluorescence often interferes with identification. Time-gated luminescence microscopy (TGLM) uses luminescent labels with long persistence in conjunction with digital imaging to regain discriminative power. Following the excitation pulse, short-lived autofluorescence decays rapidly whereas the long-lived emission from lanthanide doped polymer beads persists for hundreds of microseconds. After a short resolving period, a gated high gain camera captures the persistent emission in the absence of short-lived fluorescence. We report on the development of a TGLM software system for automated scanning of microscope slides, and show its use to resolve luminescent microspheres within a matrix of autofluorescent algae.

Original languageEnglish
Title of host publicationDICTA 2009 - Digital Image Computing: Techniques and Applications
EditorsHao Shi, Yanchun Zhang, Murk J. Bottema, Brian C. Lovell, Anthony J. Maeder
Place of PublicationPiscataway, NJ
PublisherInstitute of Electrical and Electronics Engineers (IEEE)
Pages223-228
Number of pages6
ISBN (Electronic)9780769538662
ISBN (Print)9781424452972
DOIs
Publication statusPublished - Dec 2009
EventDigital Image Computing: Techniques and Applications, DICTA - 2009 - Melbourne, Australia
Duration: 1 Dec 20093 Dec 2009

Other

OtherDigital Image Computing: Techniques and Applications, DICTA - 2009
CountryAustralia
CityMelbourne
Period1/12/093/12/09

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Bibliographical note

Copyright 2009 IEEE. Reprinted from DICTA 2009 : 2009 digital image computing techniques and applications : proceedings : 1-3 December 2009, Melbourne, Australia. This material is posted here with permission of the IEEE. Such permission of the IEEE does not in any way imply IEEE endorsement of any of Macquarie University’s products or services. Internal or personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution must be obtained from the IEEE by writing to pubs-permissions@ieee.org. By choosing to view this document, you agree to all provisions of the copyright laws protecting it.

Cite this

Hamey, L. G. C., Connally, R. E., Yen, S. W. T., Lawson, T. S., Piper, J. A., & Iredell, J. (2009). Luminescent microspheres resolved from strong background on an automated time-gated luminescence microscopy workstation. In H. Shi, Y. Zhang, M. J. Bottema, B. C. Lovell, & A. J. Maeder (Eds.), DICTA 2009 - Digital Image Computing: Techniques and Applications (pp. 223-228). [5384977] Piscataway, NJ: Institute of Electrical and Electronics Engineers (IEEE). https://doi.org/10.1109/DICTA.2009.44