Luminescent microspheres resolved from strong background on an automated time-gated luminescence microscopy workstation

Leonard G C Hamey; Russell E. Connally; Simon Wong Too Yen; Thomas S. Lawson; James A. Piper; Jon Iredell

doi:10.1109/DICTA.2009.44

Luminescent microspheres resolved from strong background on an automated time-gated luminescence microscopy workstation

Leonard G C Hamey, Russell E. Connally, Simon Wong Too Yen, Thomas S. Lawson, James A. Piper, Jon Iredell

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

2 Citations (Scopus)

71 Downloads (Pure)

Abstract

Fluorescence microscopy is a powerful tool for the rapid identification of target organisms. However, natural autofluorescence often interferes with identification. Time-gated luminescence microscopy (TGLM) uses luminescent labels with long persistence in conjunction with digital imaging to regain discriminative power. Following the excitation pulse, short-lived autofluorescence decays rapidly whereas the long-lived emission from lanthanide doped polymer beads persists for hundreds of microseconds. After a short resolving period, a gated high gain camera captures the persistent emission in the absence of short-lived fluorescence. We report on the development of a TGLM software system for automated scanning of microscope slides, and show its use to resolve luminescent microspheres within a matrix of autofluorescent algae.

Original language	English
Title of host publication	DICTA 2009 - Digital Image Computing: Techniques and Applications
Editors	Hao Shi, Yanchun Zhang, Murk J. Bottema, Brian C. Lovell, Anthony J. Maeder
Place of Publication	Piscataway, NJ
Publisher	Institute of Electrical and Electronics Engineers (IEEE)
Pages	223-228
Number of pages	6
ISBN (Electronic)	9780769538662
ISBN (Print)	9781424452972
DOIs	https://doi.org/10.1109/DICTA.2009.44
Publication status	Published - Dec 2009
Event	Digital Image Computing: Techniques and Applications, DICTA - 2009 - Melbourne, Australia Duration: 1 Dec 2009 → 3 Dec 2009

Other

Other	Digital Image Computing: Techniques and Applications, DICTA - 2009
Country/Territory	Australia
City	Melbourne
Period	1/12/09 → 3/12/09

Bibliographical note

Copyright 2009 IEEE. Reprinted from DICTA 2009 : 2009 digital image computing techniques and applications : proceedings : 1-3 December 2009, Melbourne, Australia. This material is posted here with permission of the IEEE. Such permission of the IEEE does not in any way imply IEEE endorsement of any of Macquarie Universityâ€™s products or services. Internal or personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution must be obtained from the IEEE by writing to [email protected]. By choosing to view this document, you agree to all provisions of the copyright laws protecting it.

Access to Document

10.1109/DICTA.2009.44

Publisher version (open access).pdfFinal published version, 2.85 MB

Cite this

Hamey, L. G. C., Connally, R. E., Yen, S. W. T., Lawson, T. S., Piper, J. A., & Iredell, J. (2009). Luminescent microspheres resolved from strong background on an automated time-gated luminescence microscopy workstation. In H. Shi, Y. Zhang, M. J. Bottema, B. C. Lovell, & A. J. Maeder (Eds.), DICTA 2009 - Digital Image Computing: Techniques and Applications (pp. 223-228). Article 5384977 Institute of Electrical and Electronics Engineers (IEEE). https://doi.org/10.1109/DICTA.2009.44

Hamey, Leonard G C ; Connally, Russell E. ; Yen, Simon Wong Too et al. / Luminescent microspheres resolved from strong background on an automated time-gated luminescence microscopy workstation. DICTA 2009 - Digital Image Computing: Techniques and Applications. editor / Hao Shi ; Yanchun Zhang ; Murk J. Bottema ; Brian C. Lovell ; Anthony J. Maeder. Piscataway, NJ : Institute of Electrical and Electronics Engineers (IEEE), 2009. pp. 223-228

@inproceedings{b77a694199ed42998435cdf1799bc440,

title = "Luminescent microspheres resolved from strong background on an automated time-gated luminescence microscopy workstation",

abstract = "Fluorescence microscopy is a powerful tool for the rapid identification of target organisms. However, natural autofluorescence often interferes with identification. Time-gated luminescence microscopy (TGLM) uses luminescent labels with long persistence in conjunction with digital imaging to regain discriminative power. Following the excitation pulse, short-lived autofluorescence decays rapidly whereas the long-lived emission from lanthanide doped polymer beads persists for hundreds of microseconds. After a short resolving period, a gated high gain camera captures the persistent emission in the absence of short-lived fluorescence. We report on the development of a TGLM software system for automated scanning of microscope slides, and show its use to resolve luminescent microspheres within a matrix of autofluorescent algae.",

author = "Hamey, \{Leonard G C\} and Connally, \{Russell E.\} and Yen, \{Simon Wong Too\} and Lawson, \{Thomas S.\} and Piper, \{James A.\} and Jon Iredell",

note = "Copyright 2009 IEEE. Reprinted from DICTA 2009 : 2009 digital image computing techniques and applications : proceedings : 1-3 December 2009, Melbourne, Australia. This material is posted here with permission of the IEEE. Such permission of the IEEE does not in any way imply IEEE endorsement of any of Macquarie University{\^a}€{\texttrademark}s products or services. Internal or personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution must be obtained from the IEEE by writing to [email protected]. By choosing to view this document, you agree to all provisions of the copyright laws protecting it.; Digital Image Computing: Techniques and Applications, DICTA - 2009 ; Conference date: 01-12-2009 Through 03-12-2009",

year = "2009",

month = dec,

doi = "10.1109/DICTA.2009.44",

language = "English",

isbn = "9781424452972",

pages = "223--228",

editor = "Hao Shi and Yanchun Zhang and Bottema, \{Murk J.\} and Lovell, \{Brian C.\} and Maeder, \{Anthony J.\}",

booktitle = "DICTA 2009 - Digital Image Computing: Techniques and Applications",

publisher = "Institute of Electrical and Electronics Engineers (IEEE)",

address = "United States",

}

Hamey, LGC, Connally, RE, Yen, SWT, Lawson, TS, Piper, JA & Iredell, J 2009, Luminescent microspheres resolved from strong background on an automated time-gated luminescence microscopy workstation. in H Shi, Y Zhang, MJ Bottema, BC Lovell & AJ Maeder (eds), DICTA 2009 - Digital Image Computing: Techniques and Applications., 5384977, Institute of Electrical and Electronics Engineers (IEEE), Piscataway, NJ, pp. 223-228, Digital Image Computing: Techniques and Applications, DICTA - 2009, Melbourne, Victoria, Australia, 1/12/09. https://doi.org/10.1109/DICTA.2009.44

Luminescent microspheres resolved from strong background on an automated time-gated luminescence microscopy workstation. / Hamey, Leonard G C; Connally, Russell E.; Yen, Simon Wong Too et al.
DICTA 2009 - Digital Image Computing: Techniques and Applications. ed. / Hao Shi; Yanchun Zhang; Murk J. Bottema; Brian C. Lovell; Anthony J. Maeder. Piscataway, NJ: Institute of Electrical and Electronics Engineers (IEEE), 2009. p. 223-228 5384977.

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

TY - GEN

T1 - Luminescent microspheres resolved from strong background on an automated time-gated luminescence microscopy workstation

AU - Hamey, Leonard G C

AU - Connally, Russell E.

AU - Yen, Simon Wong Too

AU - Lawson, Thomas S.

AU - Piper, James A.

AU - Iredell, Jon

N1 - Copyright 2009 IEEE. Reprinted from DICTA 2009 : 2009 digital image computing techniques and applications : proceedings : 1-3 December 2009, Melbourne, Australia. This material is posted here with permission of the IEEE. Such permission of the IEEE does not in any way imply IEEE endorsement of any of Macquarie Universityâ€™s products or services. Internal or personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution must be obtained from the IEEE by writing to [email protected]. By choosing to view this document, you agree to all provisions of the copyright laws protecting it.

PY - 2009/12

Y1 - 2009/12

N2 - Fluorescence microscopy is a powerful tool for the rapid identification of target organisms. However, natural autofluorescence often interferes with identification. Time-gated luminescence microscopy (TGLM) uses luminescent labels with long persistence in conjunction with digital imaging to regain discriminative power. Following the excitation pulse, short-lived autofluorescence decays rapidly whereas the long-lived emission from lanthanide doped polymer beads persists for hundreds of microseconds. After a short resolving period, a gated high gain camera captures the persistent emission in the absence of short-lived fluorescence. We report on the development of a TGLM software system for automated scanning of microscope slides, and show its use to resolve luminescent microspheres within a matrix of autofluorescent algae.

AB - Fluorescence microscopy is a powerful tool for the rapid identification of target organisms. However, natural autofluorescence often interferes with identification. Time-gated luminescence microscopy (TGLM) uses luminescent labels with long persistence in conjunction with digital imaging to regain discriminative power. Following the excitation pulse, short-lived autofluorescence decays rapidly whereas the long-lived emission from lanthanide doped polymer beads persists for hundreds of microseconds. After a short resolving period, a gated high gain camera captures the persistent emission in the absence of short-lived fluorescence. We report on the development of a TGLM software system for automated scanning of microscope slides, and show its use to resolve luminescent microspheres within a matrix of autofluorescent algae.

UR - https://www.scopus.com/pages/publications/77950336023

U2 - 10.1109/DICTA.2009.44

DO - 10.1109/DICTA.2009.44

M3 - Conference proceeding contribution

AN - SCOPUS:77950336023

SN - 9781424452972

SP - 223

EP - 228

BT - DICTA 2009 - Digital Image Computing: Techniques and Applications

A2 - Shi, Hao

A2 - Zhang, Yanchun

A2 - Bottema, Murk J.

A2 - Lovell, Brian C.

A2 - Maeder, Anthony J.

PB - Institute of Electrical and Electronics Engineers (IEEE)

CY - Piscataway, NJ

T2 - Digital Image Computing: Techniques and Applications, DICTA - 2009

Y2 - 1 December 2009 through 3 December 2009

ER -

Hamey LGC, Connally RE, Yen SWT, Lawson TS, Piper JA, Iredell J. Luminescent microspheres resolved from strong background on an automated time-gated luminescence microscopy workstation. In Shi H, Zhang Y, Bottema MJ, Lovell BC, Maeder AJ, editors, DICTA 2009 - Digital Image Computing: Techniques and Applications. Piscataway, NJ: Institute of Electrical and Electronics Engineers (IEEE). 2009. p. 223-228. 5384977 doi: 10.1109/DICTA.2009.44

Luminescent microspheres resolved from strong background on an automated time-gated luminescence microscopy workstation

Abstract

Other

Bibliographical note

Access to Document

Other files and links

Fingerprint

Cite this