Luminescent nanomaterials for molecular-specific cellular imaging

Andrei Vasilyevich Zvyagin, Zhen Song, Annemarie Nadort, Varun Kumaraswamy Annayya Sreenivasan, Sergey Mikhailovich Deyev

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review

Abstract

Imaging of molecular trafficking in cells and biological tissue aided by molecular-specific fluorescent labeling is very attractive, since it affords capturing the key processes in comprehensive biological context. Several shortcomings of the existing organic dye labeling technology, however, call for development of alternative molecular reporters, with improved photostability, reduced cytotoxicity, and an increased number of controllable surface moieties. Such alternative molecular reporters are represented by inorganic luminescent nanoparticles (NP) whose optical, physical, and chemical properties are discussed on the examples of luminescent nanodiamonds (LND) and upconversion nanoparticles (UCNP). The emission origins of these nanomaterials differ markedly. LND emission results from individual nitrogen-vacancy color-centers in a biocompatible nanodiamond host whose properties can be controlled via size and surface groups. Photophysics of UCNP is governed by the collective, nonlinear excitation transfer processes, resulting in conversion of longer-wavelength excitation to the shorter-wavelength emission. The emission/excitation spectral properties of UCNP falling within the biological tissue transparency window open new opportunities of almost complete suppression of the cell/tissue autofluorescence background. The developed surface of these nanoparticles represents a flexible platform populated with biocompatible surface moieties onto which cargo and targeting biomolecules can be firmly docked through a process called bioconjugation. These bioconjugated modules, e.g., nanodiamond-antibody, (quantum dot)-somatostatin, or (upconversion nanoparticle)-(mini-antibody) can gain admission into the cells by initiating the cell-specific, cell-recognized communication protocol. In this chapter, we aim to demonstrate the whole bottom-up bio-nano-optics approach for optical biological imaging capturing luminescent nanoparticle design, surface activation, and bioconjugation and the resultant bioconjugate module deployment in specific internalization in the cell.
LanguageEnglish
Title of host publicationHandbook of nano-optics and nanophotonics
EditorsMotoichi Ohtsu
Place of PublicationBerlin ; New York
PublisherSpringer, Springer Nature
Pages563-596
Number of pages34
ISBN (Print)9783642310652
DOIs
Publication statusPublished - 2013

Publication series

NameSpringer reference
PublisherSpringer

Fingerprint

Nanostructured materials
Nanodiamonds
Nanoparticles
Imaging techniques
Tissue
Labeling
Color centers
Wavelength
Antibodies
Biomolecules
Cytotoxicity
Somatostatin
Transparency
Chemical properties
Semiconductor quantum dots
Vacancies
Optics
Coloring Agents
Nitrogen
Optical properties

Cite this

Zvyagin, A. V., Song, Z., Nadort, A., Sreenivasan, V. K. A., & Deyev, S. M. (2013). Luminescent nanomaterials for molecular-specific cellular imaging. In M. Ohtsu (Ed.), Handbook of nano-optics and nanophotonics (pp. 563-596). (Springer reference). Berlin ; New York: Springer, Springer Nature. https://doi.org/10.1007/978-3-642-31066-9_15
Zvyagin, Andrei Vasilyevich ; Song, Zhen ; Nadort, Annemarie ; Sreenivasan, Varun Kumaraswamy Annayya ; Deyev, Sergey Mikhailovich. / Luminescent nanomaterials for molecular-specific cellular imaging. Handbook of nano-optics and nanophotonics. editor / Motoichi Ohtsu. Berlin ; New York : Springer, Springer Nature, 2013. pp. 563-596 (Springer reference).
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Zvyagin, AV, Song, Z, Nadort, A, Sreenivasan, VKA & Deyev, SM 2013, Luminescent nanomaterials for molecular-specific cellular imaging. in M Ohtsu (ed.), Handbook of nano-optics and nanophotonics. Springer reference, Springer, Springer Nature, Berlin ; New York, pp. 563-596. https://doi.org/10.1007/978-3-642-31066-9_15

Luminescent nanomaterials for molecular-specific cellular imaging. / Zvyagin, Andrei Vasilyevich; Song, Zhen; Nadort, Annemarie; Sreenivasan, Varun Kumaraswamy Annayya; Deyev, Sergey Mikhailovich.

Handbook of nano-optics and nanophotonics. ed. / Motoichi Ohtsu. Berlin ; New York : Springer, Springer Nature, 2013. p. 563-596 (Springer reference).

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review

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AU - Zvyagin, Andrei Vasilyevich

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AU - Deyev, Sergey Mikhailovich

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N2 - Imaging of molecular trafficking in cells and biological tissue aided by molecular-specific fluorescent labeling is very attractive, since it affords capturing the key processes in comprehensive biological context. Several shortcomings of the existing organic dye labeling technology, however, call for development of alternative molecular reporters, with improved photostability, reduced cytotoxicity, and an increased number of controllable surface moieties. Such alternative molecular reporters are represented by inorganic luminescent nanoparticles (NP) whose optical, physical, and chemical properties are discussed on the examples of luminescent nanodiamonds (LND) and upconversion nanoparticles (UCNP). The emission origins of these nanomaterials differ markedly. LND emission results from individual nitrogen-vacancy color-centers in a biocompatible nanodiamond host whose properties can be controlled via size and surface groups. Photophysics of UCNP is governed by the collective, nonlinear excitation transfer processes, resulting in conversion of longer-wavelength excitation to the shorter-wavelength emission. The emission/excitation spectral properties of UCNP falling within the biological tissue transparency window open new opportunities of almost complete suppression of the cell/tissue autofluorescence background. The developed surface of these nanoparticles represents a flexible platform populated with biocompatible surface moieties onto which cargo and targeting biomolecules can be firmly docked through a process called bioconjugation. These bioconjugated modules, e.g., nanodiamond-antibody, (quantum dot)-somatostatin, or (upconversion nanoparticle)-(mini-antibody) can gain admission into the cells by initiating the cell-specific, cell-recognized communication protocol. In this chapter, we aim to demonstrate the whole bottom-up bio-nano-optics approach for optical biological imaging capturing luminescent nanoparticle design, surface activation, and bioconjugation and the resultant bioconjugate module deployment in specific internalization in the cell.

AB - Imaging of molecular trafficking in cells and biological tissue aided by molecular-specific fluorescent labeling is very attractive, since it affords capturing the key processes in comprehensive biological context. Several shortcomings of the existing organic dye labeling technology, however, call for development of alternative molecular reporters, with improved photostability, reduced cytotoxicity, and an increased number of controllable surface moieties. Such alternative molecular reporters are represented by inorganic luminescent nanoparticles (NP) whose optical, physical, and chemical properties are discussed on the examples of luminescent nanodiamonds (LND) and upconversion nanoparticles (UCNP). The emission origins of these nanomaterials differ markedly. LND emission results from individual nitrogen-vacancy color-centers in a biocompatible nanodiamond host whose properties can be controlled via size and surface groups. Photophysics of UCNP is governed by the collective, nonlinear excitation transfer processes, resulting in conversion of longer-wavelength excitation to the shorter-wavelength emission. The emission/excitation spectral properties of UCNP falling within the biological tissue transparency window open new opportunities of almost complete suppression of the cell/tissue autofluorescence background. The developed surface of these nanoparticles represents a flexible platform populated with biocompatible surface moieties onto which cargo and targeting biomolecules can be firmly docked through a process called bioconjugation. These bioconjugated modules, e.g., nanodiamond-antibody, (quantum dot)-somatostatin, or (upconversion nanoparticle)-(mini-antibody) can gain admission into the cells by initiating the cell-specific, cell-recognized communication protocol. In this chapter, we aim to demonstrate the whole bottom-up bio-nano-optics approach for optical biological imaging capturing luminescent nanoparticle design, surface activation, and bioconjugation and the resultant bioconjugate module deployment in specific internalization in the cell.

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Zvyagin AV, Song Z, Nadort A, Sreenivasan VKA, Deyev SM. Luminescent nanomaterials for molecular-specific cellular imaging. In Ohtsu M, editor, Handbook of nano-optics and nanophotonics. Berlin ; New York: Springer, Springer Nature. 2013. p. 563-596. (Springer reference). https://doi.org/10.1007/978-3-642-31066-9_15