MALDI-TOF MS meets WGS in a VRE outbreak investigation

S. Schlebusch; G. R. Price; R. L. Gallagher; V. Horton-Szar; L. D. H. Elbourne; P. Griffin; D. J. Venter; S. O. Jensen; S. J. Van Hal

doi:10.1007/s10096-016-2824-4

MALDI-TOF MS meets WGS in a VRE outbreak investigation

S. Schlebusch^*, G. R. Price, R. L. Gallagher, V. Horton-Szar, L. D. H. Elbourne, P. Griffin, D. J. Venter, S. O. Jensen, S. J. Van Hal

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

The use of MALDI-TOF MS (matrix-assisted laser desorption/ ionization–time of flight mass spectrometry) and WGS (whole genome sequencing) has been described for identification and strain relatedness determination. We describe the complementary use of MALDI-TOF MS and WGS in a VRE (vancomycin-resistant enterococci) outbreak investigation, and discuss some of the challenges with defining strain similarity across these two platforms. Although both assays indicated multiple clusters involved in the outbreak of vancomycin resistant Enterococcus faecium isolates from positive blood cultures of four haematology–oncology patients, the small cohort and discrepancies between findings indicate the limitations of MALDI-TOF MS and the cautious interpretation of MALDI-TOF MS dendrograms during outbreaks. For definitive determination of the evolutionary distance between isolates, WGS can be used.

Original language	English
Pages (from-to)	495-499
Number of pages	5
Journal	European Journal of Clinical Microbiology and Infectious Diseases
Volume	36
Issue number	3
Early online date	26 Nov 2016
DOIs	https://doi.org/10.1007/s10096-016-2824-4
Publication status	Published - Mar 2017

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title = "MALDI-TOF MS meets WGS in a VRE outbreak investigation",

abstract = "The use of MALDI-TOF MS (matrix-assisted laser desorption/ ionization–time of flight mass spectrometry) and WGS (whole genome sequencing) has been described for identification and strain relatedness determination. We describe the complementary use of MALDI-TOF MS and WGS in a VRE (vancomycin-resistant enterococci) outbreak investigation, and discuss some of the challenges with defining strain similarity across these two platforms. Although both assays indicated multiple clusters involved in the outbreak of vancomycin resistant Enterococcus faecium isolates from positive blood cultures of four haematology–oncology patients, the small cohort and discrepancies between findings indicate the limitations of MALDI-TOF MS and the cautious interpretation of MALDI-TOF MS dendrograms during outbreaks. For definitive determination of the evolutionary distance between isolates, WGS can be used.",

author = "S. Schlebusch and Price, {G. R.} and Gallagher, {R. L.} and V. Horton-Szar and Elbourne, {L. D. H.} and P. Griffin and Venter, {D. J.} and Jensen, {S. O.} and {Van Hal}, {S. J.}",

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AU - Schlebusch, S.

AU - Price, G. R.

AU - Gallagher, R. L.

AU - Horton-Szar, V.

AU - Elbourne, L. D. H.

AU - Griffin, P.

AU - Venter, D. J.

AU - Jensen, S. O.

AU - Van Hal, S. J.

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AB - The use of MALDI-TOF MS (matrix-assisted laser desorption/ ionization–time of flight mass spectrometry) and WGS (whole genome sequencing) has been described for identification and strain relatedness determination. We describe the complementary use of MALDI-TOF MS and WGS in a VRE (vancomycin-resistant enterococci) outbreak investigation, and discuss some of the challenges with defining strain similarity across these two platforms. Although both assays indicated multiple clusters involved in the outbreak of vancomycin resistant Enterococcus faecium isolates from positive blood cultures of four haematology–oncology patients, the small cohort and discrepancies between findings indicate the limitations of MALDI-TOF MS and the cautious interpretation of MALDI-TOF MS dendrograms during outbreaks. For definitive determination of the evolutionary distance between isolates, WGS can be used.

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MALDI-TOF MS meets WGS in a VRE outbreak investigation

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