Mapping the distribution of the telomeric sequence (T 2AG 3) n in the 2n = 14 ancestral marsupial complement and in the macropodines (Marsupialia: Macropodidae) by fluorescence in situ hybridization

C. J. Metcalfe, M. D. B. Eldridge, P. G. Johnston

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23 Citations (Scopus)

Abstract

In this study we test the theory that the presence of the conserved vertebrate telomeric sequence (T 2AG 3) n at the centromeres of Australian marsupial 2n = 14 complements is evidence that these karyotypes are recently derived, which is contrary to the generally held view that the 2n = 14 karyotype is ancestral for Australasian and American marsupials. Here we compare the distribution of the (T 2AG 3) n sequence and constitutive heterochromatin in the presumed ancestral 2n = 14 complement and in complements with known rearrangements. We found that where there were moderate to large amounts of constitutive heterochromatin, the distribution of the (T 2AG 3) n sequence reflected its presence as a native component of satellite DNA rather than its involvement in past rearrangements. The presence of centromeric heterochromatin in all Australian 2n = 14 complements therefore suggests that centromeric sites of the (T 2AG 3) n sequence do not represent evidence for recent rearrangements.

Original languageEnglish
Pages (from-to)405-414
Number of pages10
JournalChromosome Research
Volume12
Issue number4
DOIs
Publication statusPublished - 2004

Keywords

  • fluorescence in-situ hybridization
  • karyology
  • Macropodidae
  • marsupial
  • telomere

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