Mapping the distribution of the telomeric sequence (T 2AG 3) n in the 2n = 14 ancestral marsupial complement and in the macropodines (Marsupialia: Macropodidae) by fluorescence in situ hybridization

C. J. Metcalfe, M. D. B. Eldridge, P. G. Johnston

    Research output: Contribution to journalArticlepeer-review

    25 Citations (Scopus)

    Abstract

    In this study we test the theory that the presence of the conserved vertebrate telomeric sequence (T 2AG 3) n at the centromeres of Australian marsupial 2n = 14 complements is evidence that these karyotypes are recently derived, which is contrary to the generally held view that the 2n = 14 karyotype is ancestral for Australasian and American marsupials. Here we compare the distribution of the (T 2AG 3) n sequence and constitutive heterochromatin in the presumed ancestral 2n = 14 complement and in complements with known rearrangements. We found that where there were moderate to large amounts of constitutive heterochromatin, the distribution of the (T 2AG 3) n sequence reflected its presence as a native component of satellite DNA rather than its involvement in past rearrangements. The presence of centromeric heterochromatin in all Australian 2n = 14 complements therefore suggests that centromeric sites of the (T 2AG 3) n sequence do not represent evidence for recent rearrangements.

    Original languageEnglish
    Pages (from-to)405-414
    Number of pages10
    JournalChromosome Research
    Volume12
    Issue number4
    DOIs
    Publication statusPublished - 2004

    Keywords

    • fluorescence in-situ hybridization
    • karyology
    • Macropodidae
    • marsupial
    • telomere

    Fingerprint

    Dive into the research topics of 'Mapping the distribution of the telomeric sequence (T 2AG 3) n in the 2n = 14 ancestral marsupial complement and in the macropodines (Marsupialia: Macropodidae) by fluorescence in situ hybridization'. Together they form a unique fingerprint.

    Cite this