Marker assisted selection for heat tolerance in bread wheat

Shahab Sadat*, Khalil Alami Saeid, Mohammad Reza Bihamta, Sepideh Torabi, Seyed Ghasem Hoseini Salekdeh, Gholam Abbas Lotfali Ayeneh

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Due to difficulty of managing of heat tolerance through conventional phenotypic selection and the presence of several QTLs for such a polygenic trait with complex inheritance, Marker Assisted Selection (MAS) was considered as an efficient method for screening 25 bread wheat genotypes to heat stress. Fourteen SSR markers which were linked to some important traits including grain filling duration, HSI (Heat Susceptibility Index) grain filling duration, HSI_single kernel weight of main spike and HSI kernel weight under heat stress were selected from previous reports. After DNA extraction and running PCR products on 8% acrylamide gel and PeqGOLD MoSieve Agarose MS-1000 and staining procedure, data scoring and statistical analysis were performed. Constructed dendrogram using the Unweighted Pair-Group Method on Arithmetic mean (UPGMA) grouped genotypes in 3 clusters. Two genotypes were laid in a same cluster. One genotype was grouped in another cluster and the remaining 22 genotypes were laid in the third cluster. The results of field experiments from previous reports and the results of molecular screening for heat tolerance indicated that selected markers were able to screen bread wheat genotypes for heat tolerance.

Original languageEnglish
Pages (from-to)1181-1189
Number of pages9
JournalWorld Applied Sciences Journal
Volume21
Issue number8
DOIs
Publication statusPublished - 2013
Externally publishedYes

Keywords

  • marker assisted selection
  • heat stress
  • wheat
  • molecular markers

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