TY - JOUR
T1 - Marker assisted selection for heat tolerance in bread wheat
AU - Sadat, Shahab
AU - Saeid, Khalil Alami
AU - Bihamta, Mohammad Reza
AU - Torabi, Sepideh
AU - Hoseini Salekdeh, Seyed Ghasem
AU - Lotfali Ayeneh, Gholam Abbas
PY - 2013
Y1 - 2013
N2 - Due to difficulty of managing of heat tolerance through conventional phenotypic selection and the presence of several QTLs for such a polygenic trait with complex inheritance, Marker Assisted Selection (MAS) was considered as an efficient method for screening 25 bread wheat genotypes to heat stress. Fourteen SSR markers which were linked to some important traits including grain filling duration, HSI (Heat Susceptibility Index) grain filling duration, HSI_single kernel weight of main spike and HSI kernel weight under heat stress were selected from previous reports. After DNA extraction and running PCR products on 8% acrylamide gel and PeqGOLD MoSieve Agarose MS-1000 and staining procedure, data scoring and statistical analysis were performed. Constructed dendrogram using the Unweighted Pair-Group Method on Arithmetic mean (UPGMA) grouped genotypes in 3 clusters. Two genotypes were laid in a same cluster. One genotype was grouped in another cluster and the remaining 22 genotypes were laid in the third cluster. The results of field experiments from previous reports and the results of molecular screening for heat tolerance indicated that selected markers were able to screen bread wheat genotypes for heat tolerance.
AB - Due to difficulty of managing of heat tolerance through conventional phenotypic selection and the presence of several QTLs for such a polygenic trait with complex inheritance, Marker Assisted Selection (MAS) was considered as an efficient method for screening 25 bread wheat genotypes to heat stress. Fourteen SSR markers which were linked to some important traits including grain filling duration, HSI (Heat Susceptibility Index) grain filling duration, HSI_single kernel weight of main spike and HSI kernel weight under heat stress were selected from previous reports. After DNA extraction and running PCR products on 8% acrylamide gel and PeqGOLD MoSieve Agarose MS-1000 and staining procedure, data scoring and statistical analysis were performed. Constructed dendrogram using the Unweighted Pair-Group Method on Arithmetic mean (UPGMA) grouped genotypes in 3 clusters. Two genotypes were laid in a same cluster. One genotype was grouped in another cluster and the remaining 22 genotypes were laid in the third cluster. The results of field experiments from previous reports and the results of molecular screening for heat tolerance indicated that selected markers were able to screen bread wheat genotypes for heat tolerance.
KW - marker assisted selection
KW - heat stress
KW - wheat
KW - molecular markers
UR - http://www.scopus.com/inward/record.url?scp=84875039881&partnerID=8YFLogxK
UR - https://www.idosi.org/wasj/wasj21(8)2013.htm
U2 - 10.5829/idosi.wasj.2013.21.8.2866
DO - 10.5829/idosi.wasj.2013.21.8.2866
M3 - Article
AN - SCOPUS:84875039881
SN - 1818-4952
VL - 21
SP - 1181
EP - 1189
JO - World Applied Sciences Journal
JF - World Applied Sciences Journal
IS - 8
ER -