Mass spectral compatibility of four proteomics stains

Malcolm S. Ball, Peter Karuso

Research output: Contribution to journalArticleResearchpeer-review

Abstract

With the recent introduction of new fluorescence stains to the proteomics market, there is now more choice available. SYPRO Ruby, LavaPurple, Flamingo, and Krypton total protein stains were compared for ease of use, image quality, and compatibility with protein identification by peptide mass fingerprinting (PMF) (MALDI-TOF). All four stains produced good images but with slightly different staining patterns. SYPRO was found to inhibit identification of cysteine and tryptophan containing peptides, which reduced protein identification.

LanguageEnglish
Pages4313-4320
Number of pages8
JournalJournal of Proteome Research
Volume6
Issue number11
DOIs
Publication statusPublished - Nov 2007

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Proteomics
Coloring Agents
Krypton
Ruby
Peptides
Proteins
Peptide Mapping
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Tryptophan
Image quality
Cysteine
Fluorescence
Staining and Labeling
stains-all

Cite this

Ball, Malcolm S. ; Karuso, Peter. / Mass spectral compatibility of four proteomics stains. In: Journal of Proteome Research. 2007 ; Vol. 6, No. 11. pp. 4313-4320.
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Mass spectral compatibility of four proteomics stains. / Ball, Malcolm S.; Karuso, Peter.

In: Journal of Proteome Research, Vol. 6, No. 11, 11.2007, p. 4313-4320.

Research output: Contribution to journalArticleResearchpeer-review

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