Mass spectral compatibility of four proteomics stains

Malcolm S. Ball, Peter Karuso*

*Corresponding author for this work

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

With the recent introduction of new fluorescence stains to the proteomics market, there is now more choice available. SYPRO Ruby, LavaPurple, Flamingo, and Krypton total protein stains were compared for ease of use, image quality, and compatibility with protein identification by peptide mass fingerprinting (PMF) (MALDI-TOF). All four stains produced good images but with slightly different staining patterns. SYPRO was found to inhibit identification of cysteine and tryptophan containing peptides, which reduced protein identification.

Original languageEnglish
Pages (from-to)4313-4320
Number of pages8
JournalJournal of Proteome Research
Volume6
Issue number11
DOIs
Publication statusPublished - Nov 2007

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