Mass spectral compatibility of four proteomics stains

Malcolm S. Ball, Peter Karuso*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    24 Citations (Scopus)

    Abstract

    With the recent introduction of new fluorescence stains to the proteomics market, there is now more choice available. SYPRO Ruby, LavaPurple, Flamingo, and Krypton total protein stains were compared for ease of use, image quality, and compatibility with protein identification by peptide mass fingerprinting (PMF) (MALDI-TOF). All four stains produced good images but with slightly different staining patterns. SYPRO was found to inhibit identification of cysteine and tryptophan containing peptides, which reduced protein identification.

    Original languageEnglish
    Pages (from-to)4313-4320
    Number of pages8
    JournalJournal of Proteome Research
    Volume6
    Issue number11
    DOIs
    Publication statusPublished - Nov 2007

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