TY - JOUR
T1 - Mass spectrometric analysis of the editosome and other multiprotein complexes in Trypanosoma brucei
AU - Panigrahi, Aswini K.
AU - Allen, Thomas E.
AU - Stuart, Kenneth
AU - Haynes, Paul A.
AU - Gygi, Steven P.
PY - 2003/7
Y1 - 2003/7
N2 - The composition of the editosome, a multi-protein complex that catalyzes uridine insertion and deletion RNA editing to produce mature mitochondrial mRNAs in trypanosomes, was analyzed by mass spectrometry. The editosomes were isolated by column chromatography, glycerol gradient sedimentation, and monoclonal antibody affinity purifications. At least 16 proteins form the catalytic core of the editosome, and additional associated proteins were identified. Analyses of mitochondrial fractions identified several non-editosome proteins and multi-protein complexes. These studies contribute to the functional annotation of T. brucei genome.
AB - The composition of the editosome, a multi-protein complex that catalyzes uridine insertion and deletion RNA editing to produce mature mitochondrial mRNAs in trypanosomes, was analyzed by mass spectrometry. The editosomes were isolated by column chromatography, glycerol gradient sedimentation, and monoclonal antibody affinity purifications. At least 16 proteins form the catalytic core of the editosome, and additional associated proteins were identified. Analyses of mitochondrial fractions identified several non-editosome proteins and multi-protein complexes. These studies contribute to the functional annotation of T. brucei genome.
UR - http://www.scopus.com/inward/record.url?scp=0038488182&partnerID=8YFLogxK
U2 - 10.1016/S1044-0305(03)00126-0
DO - 10.1016/S1044-0305(03)00126-0
M3 - Article
C2 - 12837594
AN - SCOPUS:0038488182
SN - 1044-0305
VL - 14
SP - 728
EP - 735
JO - Journal of the American Society for Mass Spectrometry
JF - Journal of the American Society for Mass Spectrometry
IS - 7
ER -