TY - JOUR
T1 - Mass spectrometric identification of indole compounds produced by Rhizobium strains
AU - Badenoch‐Jones, J.
AU - Summons, R. E.
AU - Entsch, B.
AU - Rolfe, B. G.
AU - Parker, C. W.
AU - Letham, D. S.
PY - 1982
Y1 - 1982
N2 - The following indole compounds have been unequivocally identified and semi‐quantitated in culture supernatants of Rhizobium strains by gas chromatography mass spectrometry: indole‐3‐aldehyde, trytophol, indole‐3‐carboxylic acid, indole‐3‐glycollc acid, indole‐3‐actic acid, indole‐3‐glyoxylic acid, N‐acethyl‐L‐tryptophan and indole‐3‐pyruvic acid, as well as indole‐3‐acetic acid. The strains included a number of chosen Rhizobium mutants defective in various stages of nodule formation, and the biological implications of the findings are discussed. Two alternative approaches to sample purification were taken, one based primarily on solvent partitioning and the other on high‐performance liquid chromatography. The advantages of each method are discussed. Gas chromatographic retention times and mass spectra are given for the TMS derivatives of 17 authentic indole standards.
AB - The following indole compounds have been unequivocally identified and semi‐quantitated in culture supernatants of Rhizobium strains by gas chromatography mass spectrometry: indole‐3‐aldehyde, trytophol, indole‐3‐carboxylic acid, indole‐3‐glycollc acid, indole‐3‐actic acid, indole‐3‐glyoxylic acid, N‐acethyl‐L‐tryptophan and indole‐3‐pyruvic acid, as well as indole‐3‐acetic acid. The strains included a number of chosen Rhizobium mutants defective in various stages of nodule formation, and the biological implications of the findings are discussed. Two alternative approaches to sample purification were taken, one based primarily on solvent partitioning and the other on high‐performance liquid chromatography. The advantages of each method are discussed. Gas chromatographic retention times and mass spectra are given for the TMS derivatives of 17 authentic indole standards.
UR - http://www.scopus.com/inward/record.url?scp=0020470720&partnerID=8YFLogxK
U2 - 10.1002/bms.1200091005
DO - 10.1002/bms.1200091005
M3 - Article
AN - SCOPUS:0020470720
SN - 0306-042X
VL - 9
SP - 429
EP - 437
JO - Biological Mass Spectrometry
JF - Biological Mass Spectrometry
IS - 10
ER -