TY - JOUR
T1 - Maturing glycoproteomics technologies provide unique structural insights into the N-glycoproteome and its regulation in health and disease
AU - Thaysen-Andersen, Morten
AU - Packer, Nicolle H.
AU - Schulz, Benjamin L.
PY - 2016/6/1
Y1 - 2016/6/1
N2 - The glycoproteome remains severely understudied because of significant analytical challenges associated with glycoproteomics, the system-wide analysis of intact glycopeptides. This review introduces important structural aspects of protein N-glycosylation and summarizes the latest technological developments and applications in LC-MS/MS-based qualitative and quantitative N-glycoproteomics. These maturing technologies provide unique structural insights into the N-glycoproteome and its synthesis and regulation by complementing existing methods in glycoscience. Modern glycoproteomics is now sufficiently mature to initiate efforts to capture the molecular complexity displayed by the N-glycoproteome, opening exciting opportunities to increase our understanding of the functional roles of protein N-glycosylation in human health and disease.
AB - The glycoproteome remains severely understudied because of significant analytical challenges associated with glycoproteomics, the system-wide analysis of intact glycopeptides. This review introduces important structural aspects of protein N-glycosylation and summarizes the latest technological developments and applications in LC-MS/MS-based qualitative and quantitative N-glycoproteomics. These maturing technologies provide unique structural insights into the N-glycoproteome and its synthesis and regulation by complementing existing methods in glycoscience. Modern glycoproteomics is now sufficiently mature to initiate efforts to capture the molecular complexity displayed by the N-glycoproteome, opening exciting opportunities to increase our understanding of the functional roles of protein N-glycosylation in human health and disease.
UR - http://www.scopus.com/inward/record.url?scp=84964949185&partnerID=8YFLogxK
UR - http://purl.org/au-research/grants/arc/CE140100003
U2 - 10.1074/mcp.O115.057638
DO - 10.1074/mcp.O115.057638
M3 - Review article
C2 - 26929216
AN - SCOPUS:84964949185
SN - 1535-9476
VL - 15
SP - 1773
EP - 1790
JO - Molecular and Cellular Proteomics
JF - Molecular and Cellular Proteomics
IS - 6
ER -