Maturing glycoproteomics technologies provide unique structural insights into the N-glycoproteome and its regulation in health and disease

Morten Thaysen-Andersen*, Nicolle H. Packer, Benjamin L. Schulz

*Corresponding author for this work

Research output: Contribution to journalReview article

69 Citations (Scopus)

Abstract

The glycoproteome remains severely understudied because of significant analytical challenges associated with glycoproteomics, the system-wide analysis of intact glycopeptides. This review introduces important structural aspects of protein N-glycosylation and summarizes the latest technological developments and applications in LC-MS/MS-based qualitative and quantitative N-glycoproteomics. These maturing technologies provide unique structural insights into the N-glycoproteome and its synthesis and regulation by complementing existing methods in glycoscience. Modern glycoproteomics is now sufficiently mature to initiate efforts to capture the molecular complexity displayed by the N-glycoproteome, opening exciting opportunities to increase our understanding of the functional roles of protein N-glycosylation in human health and disease.

Original languageEnglish
Pages (from-to)1773-1790
Number of pages18
JournalMolecular and Cellular Proteomics
Volume15
Issue number6
DOIs
Publication statusPublished - 1 Jun 2016

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