Mechanism of reversible fluorescent staining of protein with epicocconone

Daniel R. Coghlan, James A. Mackintosh, Peter Karuso

Research output: Contribution to journalArticleResearchpeer-review

Abstract

(Chemical Equation Presented) Epicocconone is the active ingredient in Deep Purple Total Protein Stain and responsible for the apparent noncovalent staining of proteins in polyacrylamide gel and electroblots. Reaction of epicocconone with amines has shown that epicocconone reacts reversibly with primary amines to produce a highly fluorescent enamine that is readily hydrolyzed by base or strong acid such as in conditions used in post-electrophoretic analysis such as peptide mass fingerprinting or Edman degradation.

LanguageEnglish
Pages2401-2404
Number of pages4
JournalOrganic Letters
Volume7
Issue number12
DOIs
Publication statusPublished - 9 Jun 2005

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staining
amines
Staining and Labeling
proteins
Amines
ingredients
peptides
Proteins
Peptide Mapping
gels
degradation
acids
Coloring Agents
Degradation
Peptides
Acids
epicocconone

Cite this

Coghlan, Daniel R. ; Mackintosh, James A. ; Karuso, Peter. / Mechanism of reversible fluorescent staining of protein with epicocconone. In: Organic Letters. 2005 ; Vol. 7, No. 12. pp. 2401-2404.
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Mechanism of reversible fluorescent staining of protein with epicocconone. / Coghlan, Daniel R.; Mackintosh, James A.; Karuso, Peter.

In: Organic Letters, Vol. 7, No. 12, 09.06.2005, p. 2401-2404.

Research output: Contribution to journalArticleResearchpeer-review

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