Mechanism of reversible fluorescent staining of protein with epicocconone

Daniel R. Coghlan; James A. Mackintosh; Peter Karuso

doi:10.1021/ol050665b

Mechanism of reversible fluorescent staining of protein with epicocconone

Daniel R. Coghlan, James A. Mackintosh, Peter Karuso^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

65 Citations (Scopus)

Abstract

(Chemical Equation Presented) Epicocconone is the active ingredient in Deep Purple Total Protein Stain and responsible for the apparent noncovalent staining of proteins in polyacrylamide gel and electroblots. Reaction of epicocconone with amines has shown that epicocconone reacts reversibly with primary amines to produce a highly fluorescent enamine that is readily hydrolyzed by base or strong acid such as in conditions used in post-electrophoretic analysis such as peptide mass fingerprinting or Edman degradation.

Original language	English
Pages (from-to)	2401-2404
Number of pages	4
Journal	Organic Letters
Volume	7
Issue number	12
DOIs	https://doi.org/10.1021/ol050665b
Publication status	Published - 9 Jun 2005

Access to Document

10.1021/ol050665b

Cite this

@article{92a98bba08ab4334b871f66234fe599a,

title = "Mechanism of reversible fluorescent staining of protein with epicocconone",

abstract = "(Chemical Equation Presented) Epicocconone is the active ingredient in Deep Purple Total Protein Stain and responsible for the apparent noncovalent staining of proteins in polyacrylamide gel and electroblots. Reaction of epicocconone with amines has shown that epicocconone reacts reversibly with primary amines to produce a highly fluorescent enamine that is readily hydrolyzed by base or strong acid such as in conditions used in post-electrophoretic analysis such as peptide mass fingerprinting or Edman degradation.",

author = "Coghlan, {Daniel R.} and Mackintosh, {James A.} and Peter Karuso",

year = "2005",

month = jun,

day = "9",

doi = "10.1021/ol050665b",

language = "English",

volume = "7",

pages = "2401--2404",

journal = "Organic Letters",

issn = "1523-7060",

publisher = "American Chemical Society",

number = "12",

}

TY - JOUR

T1 - Mechanism of reversible fluorescent staining of protein with epicocconone

AU - Coghlan, Daniel R.

AU - Mackintosh, James A.

AU - Karuso, Peter

PY - 2005/6/9

Y1 - 2005/6/9

N2 - (Chemical Equation Presented) Epicocconone is the active ingredient in Deep Purple Total Protein Stain and responsible for the apparent noncovalent staining of proteins in polyacrylamide gel and electroblots. Reaction of epicocconone with amines has shown that epicocconone reacts reversibly with primary amines to produce a highly fluorescent enamine that is readily hydrolyzed by base or strong acid such as in conditions used in post-electrophoretic analysis such as peptide mass fingerprinting or Edman degradation.

AB - (Chemical Equation Presented) Epicocconone is the active ingredient in Deep Purple Total Protein Stain and responsible for the apparent noncovalent staining of proteins in polyacrylamide gel and electroblots. Reaction of epicocconone with amines has shown that epicocconone reacts reversibly with primary amines to produce a highly fluorescent enamine that is readily hydrolyzed by base or strong acid such as in conditions used in post-electrophoretic analysis such as peptide mass fingerprinting or Edman degradation.

UR - http://www.scopus.com/inward/record.url?scp=20544457120&partnerID=8YFLogxK

U2 - 10.1021/ol050665b

DO - 10.1021/ol050665b

M3 - Article

C2 - 15932208

AN - SCOPUS:20544457120

SN - 1523-7060

VL - 7

SP - 2401

EP - 2404

JO - Organic Letters

JF - Organic Letters

IS - 12

ER -

Mechanism of reversible fluorescent staining of protein with epicocconone

Abstract

Access to Document

Other files and links

Fingerprint

Cite this