Mechanism of reversible fluorescent staining of protein with epicocconone

Daniel R. Coghlan; James A. Mackintosh; Peter Karuso

doi:10.1021/ol050665b

Mechanism of reversible fluorescent staining of protein with epicocconone

Daniel R. Coghlan, James A. Mackintosh, Peter Karuso^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

64 Citations (Scopus)

Abstract

(Chemical Equation Presented) Epicocconone is the active ingredient in Deep Purple Total Protein Stain and responsible for the apparent noncovalent staining of proteins in polyacrylamide gel and electroblots. Reaction of epicocconone with amines has shown that epicocconone reacts reversibly with primary amines to produce a highly fluorescent enamine that is readily hydrolyzed by base or strong acid such as in conditions used in post-electrophoretic analysis such as peptide mass fingerprinting or Edman degradation.

Original language	English
Pages (from-to)	2401-2404
Number of pages	4
Journal	Organic Letters
Volume	7
Issue number	12
DOIs	https://doi.org/10.1021/ol050665b
Publication status	Published - 9 Jun 2005

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10.1021/ol050665b

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abstract = "(Chemical Equation Presented) Epicocconone is the active ingredient in Deep Purple Total Protein Stain and responsible for the apparent noncovalent staining of proteins in polyacrylamide gel and electroblots. Reaction of epicocconone with amines has shown that epicocconone reacts reversibly with primary amines to produce a highly fluorescent enamine that is readily hydrolyzed by base or strong acid such as in conditions used in post-electrophoretic analysis such as peptide mass fingerprinting or Edman degradation.",

author = "Coghlan, {Daniel R.} and Mackintosh, {James A.} and Peter Karuso",

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AU - Mackintosh, James A.

AU - Karuso, Peter

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N2 - (Chemical Equation Presented) Epicocconone is the active ingredient in Deep Purple Total Protein Stain and responsible for the apparent noncovalent staining of proteins in polyacrylamide gel and electroblots. Reaction of epicocconone with amines has shown that epicocconone reacts reversibly with primary amines to produce a highly fluorescent enamine that is readily hydrolyzed by base or strong acid such as in conditions used in post-electrophoretic analysis such as peptide mass fingerprinting or Edman degradation.

AB - (Chemical Equation Presented) Epicocconone is the active ingredient in Deep Purple Total Protein Stain and responsible for the apparent noncovalent staining of proteins in polyacrylamide gel and electroblots. Reaction of epicocconone with amines has shown that epicocconone reacts reversibly with primary amines to produce a highly fluorescent enamine that is readily hydrolyzed by base or strong acid such as in conditions used in post-electrophoretic analysis such as peptide mass fingerprinting or Edman degradation.

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Mechanism of reversible fluorescent staining of protein with epicocconone

Abstract

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