Mechanism of reversible fluorescent staining of protein with epicocconone

Daniel R. Coghlan, James A. Mackintosh, Peter Karuso*

*Corresponding author for this work

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

(Chemical Equation Presented) Epicocconone is the active ingredient in Deep Purple Total Protein Stain and responsible for the apparent noncovalent staining of proteins in polyacrylamide gel and electroblots. Reaction of epicocconone with amines has shown that epicocconone reacts reversibly with primary amines to produce a highly fluorescent enamine that is readily hydrolyzed by base or strong acid such as in conditions used in post-electrophoretic analysis such as peptide mass fingerprinting or Edman degradation.

Original languageEnglish
Pages (from-to)2401-2404
Number of pages4
JournalOrganic Letters
Volume7
Issue number12
DOIs
Publication statusPublished - 9 Jun 2005

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