Mechanism of reversible fluorescent staining of protein with epicocconone

Daniel R. Coghlan, James A. Mackintosh, Peter Karuso*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    64 Citations (Scopus)


    (Chemical Equation Presented) Epicocconone is the active ingredient in Deep Purple Total Protein Stain and responsible for the apparent noncovalent staining of proteins in polyacrylamide gel and electroblots. Reaction of epicocconone with amines has shown that epicocconone reacts reversibly with primary amines to produce a highly fluorescent enamine that is readily hydrolyzed by base or strong acid such as in conditions used in post-electrophoretic analysis such as peptide mass fingerprinting or Edman degradation.

    Original languageEnglish
    Pages (from-to)2401-2404
    Number of pages4
    JournalOrganic Letters
    Issue number12
    Publication statusPublished - 9 Jun 2005


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