Melanogenesis stimulation in B16-F10 melanoma cells induces cell cycle alterations, increased ROS levels and a differential expression of proteins as revealed by proteomic analysis

Elizabeth S. Cunha, Rebeca Kawahara, Marina K. Kadowaki, Hudson G. Amstalden, Guilhermina R. Noleto, Silvia Maria S. C. Cadena, Sheila M. B. Winnischofer, Glaucia R. Martinez

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Considering that stimulation of melanogenesis may lead to alterations of cellular responses, besides melanin production, our main goal was to study the cellular effects of melanogenesis stimulation of B16-F10 melanoma cells. Our results show increased levels of the reactive oxygen species after 15 h of melanogenesis stimulation. Following 48 h of melanogenesis stimulation, proliferation was inhibited (by induction of cell cycle arrest in the G1 phase) and the expression levels of p21 mRNA were increased. In addition, melanogenesis stimulation did not induce cellular senescence. Proteomic analysis demonstrated the involvement of proteins from other pathways besides those related to the cell cycle, including protein disulfide isomerase A3, heat-shock protein 70, and fructose biphosphate aldolase A (all up-regulated), and lactate dehydrogenase (down-regulated). In RT-qPCR experiments, the levels of pyruvate kinase M2 mRNA dropped, whereas the levels of ATP synthase (beta-F1) mRNA increased. These data indicate that melanogenesis stimulation of B16-F10 cells leads to alterations in metabolism and cell cycle progression that may contribute to an induction of cell quiescence, which may provide a mechanism of resistance against cellular injury promoted by melanin synthesis.
Original languageEnglish
Pages (from-to)1913-1925
JournalExperimental Cell Research
Volume318
Issue number15
DOIs
Publication statusPublished - 2012
Externally publishedYes

Keywords

  • melanin
  • melanogenesis
  • cell cycle
  • melanoma
  • ROS

Fingerprint Dive into the research topics of 'Melanogenesis stimulation in B16-F10 melanoma cells induces cell cycle alterations, increased ROS levels and a differential expression of proteins as revealed by proteomic analysis'. Together they form a unique fingerprint.

Cite this