Membrane interaction of Pasteurella multocida toxin involves sphingomyelin

Michael C. Brothers, Mengfei Ho, Ram Maharjan, Nathan C. Clemons, Yuka Bannai, Mark A. Waites, Melinda J. Faulkner, Theresa B. Kuhlenschmidt, Mark S. Kuhlenschmidt, Steven R. Blanke, Chad M. Rienstra, Brenda A. Wilson

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Pasteurella multocida toxin (PMT) is an AB toxin that causes pleiotropic effects in targeted host cells. The N-terminus of PMT (PMT-N) is considered to harbor the membrane receptor binding and translocation domains responsible for mediating cellular entry and delivery of the C-terminal catalytic domain into the host cytosol. Previous studies have implicated gangliosides as the host receptors for PMT binding. To gain further insight into the binding interactions involved in PMT binding to cell membranes, we explored the role of various membrane components in PMT binding, utilizing four different approaches: (a) TLC-overlay binding experiments with 125I-labeled PMT, PMT-N or the C-terminus of PMT; (b) pull-down experiments using reconstituted membrane liposomes with full-length PMT; (c) surface plasmon resonance analysis of PMT-N binding to reconstituted membrane liposomes; (d) and surface plasmon resonance analysis of PMT-N binding to HEK-293T cell membranes without or with sphingomyelinase, phospholipase D or trypsin treatment. The results obtained revealed that, in our experimental system, full-length PMT and PMT-N did not bind to gangliosides, including monoasialogangliosides GM1, GM2 or GM3, but instead bound to membrane phospholipids, primarily the abundant sphingophospholipid sphingomyelin or phosphatidylcholine with other lipid components. Collectively, these studies demonstrate the importance of sphingomyelin for PMT binding to membranes and suggest the involvement of a protein co-receptor.

Original languageEnglish
Pages (from-to)4633-4648
Number of pages16
JournalFEBS Journal
Volume278
Issue number23
DOIs
Publication statusPublished - Dec 2011
Externally publishedYes

Bibliographical note

© 2011 The Authors Journal compilation © 2011 FEBS.

Keywords

  • Animals
  • Bacterial Proteins/chemistry
  • Bacterial Toxins/chemistry
  • Binding Sites
  • Cell Line
  • Cell Membrane/metabolism
  • Chlorocebus aethiops
  • Humans
  • Mice
  • Pasteurella multocida/chemistry
  • Sphingomyelins/chemistry
  • Surface Plasmon Resonance

Fingerprint Dive into the research topics of 'Membrane interaction of <i>Pasteurella multocida</i> toxin involves sphingomyelin'. Together they form a unique fingerprint.

Cite this