Metabolic and transcriptional response of central metabolism affected by root endophytic fungus Piriformospora indica under salinity in barley

Mohammad Reza Ghaffari, Mehdi Ghabooli, Behnam Khatabi, Mohammad Reza Hajirezaei, Patrick Schweizer, Ghasem Hosseini Salekdeh*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

The root endophytic fungus Piriformospora indica enhances plant adaptation to environmental stress based on general and non-specific plant species mechanisms. In the present study, we integrated the ionomics, metabolomics, and transcriptomics data to identify the genes and metabolic regulatory networks conferring salt tolerance in P. indica-colonized barley plants. To this end, leaf samples were harvested at control (0 mM NaCl) and severe salt stress (300 mM NaCl) in P. indica-colonized and non-inoculated barley plants 4 weeks after fungal inoculation. The metabolome analysis resulted in an identification of a signature containing 14 metabolites and ions conferring tolerance to salt stress. Gene expression analysis has led to the identification of 254 differentially expressed genes at 0 mM NaCl and 391 genes at 300 mM NaCl in P. indica-colonized compared to non-inoculated samples. The integration of metabolome and transcriptome analysis indicated that the major and minor carbohydrate metabolism, nitrogen metabolism, and ethylene biosynthesis pathway might play a role in systemic salt-tolerance in leaf tissue induced by the root-colonized fungus.

Original languageEnglish
Pages (from-to)699-717
Number of pages19
JournalPlant Molecular Biology
Volume90
Issue number6
DOIs
Publication statusPublished - 1 Apr 2016
Externally publishedYes

Keywords

  • Endophyte
  • Metabolomics
  • Salt stress tolerance
  • Transcriptomics

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