Metal-organic-framework-based enzymatic microfluidic biosensor via surface patterning and biomineralization

Munirah Mohammad, Amir Razmjou, Kang Liang, Mohsen Asadnia, Vicki Chen

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Recently, the biomineralization of enzyme in metal-organic-framework (enzyme-MOF) composite have shown a great potential to increase enzymes stability without compromising their activity; hence, it is desirable for its applications in biosensing devices. Nonetheless, most of the enzyme-MOF research has been focusing on enzyme encapsulation in particle form, which limits its synthesis flexibility for practical applications because of its requirement for postsynthesis immobilization onto solid support. Therefore, to develop a diagnostic device out of the biomineralized enzyme, surface patterning and integration of microfluidic system offers many advantages. In this work, mussel-inspired polydopamine/polyethyleneimine (PDA/PEI) coating is employed to pattern enzyme-MOF in microfluidic channels and exploit the wettability gradient for "pumpless transportation" effect. As a proof of concept, we combine a cascade reaction of glucose oxidase (GOx) and horseradish peroxidase (HRP) enzymes to detect glucose into a patterned zeolitic imidazole framework-8 (ZIF-8) thin film on a flexible polymeric substrate. The results show that the ZIF-8/GOx&HRP in situ composites on PDA/PEI patterns have good acid and thermal stability compared with samples without ZIF-8. ZIF-8/GOx&HRP in situ shows high selectivity toward glucose, linear sensitivity of 0.00303 Abs/μM, and the limit of detection of 8 μM glucose concentration. An unexpected benefit of this approach is the ability of the ZIF-8 thin-film structure to provide a diffusion limiting effect for substrate influx, thus, producing high range of linear response range (8 μM to 5 mM of glucose). This work provides insights into the spatial location of the enzymes in MOF thin films and the potential of such patterning techniques for MOF-based biosensors using other types of biological elements such as antibodies and aptamers.

LanguageEnglish
Pages1807-1820
Number of pages14
JournalACS Applied Materials and Interfaces
Volume11
Issue number2
DOIs
Publication statusPublished - 2019

Fingerprint

Biomineralization
Microfluidics
Biosensors
Enzymes
Metals
Glucose Oxidase
Glucose oxidase
Glucose
Horseradish Peroxidase
Polyethyleneimine
Thin films
Composite materials
Substrates
Encapsulation
Antibodies
Wetting
Thermodynamic stability
imidazole
Coatings
Acids

Keywords

  • microfluidic
  • biosensors
  • metal-organic framework
  • patterning
  • enzymes
  • polydopamine
  • pumpless transportation

Cite this

Mohammad, Munirah ; Razmjou, Amir ; Liang, Kang ; Asadnia, Mohsen ; Chen, Vicki. / Metal-organic-framework-based enzymatic microfluidic biosensor via surface patterning and biomineralization. In: ACS Applied Materials and Interfaces. 2019 ; Vol. 11, No. 2. pp. 1807-1820.
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abstract = "Recently, the biomineralization of enzyme in metal-organic-framework (enzyme-MOF) composite have shown a great potential to increase enzymes stability without compromising their activity; hence, it is desirable for its applications in biosensing devices. Nonetheless, most of the enzyme-MOF research has been focusing on enzyme encapsulation in particle form, which limits its synthesis flexibility for practical applications because of its requirement for postsynthesis immobilization onto solid support. Therefore, to develop a diagnostic device out of the biomineralized enzyme, surface patterning and integration of microfluidic system offers many advantages. In this work, mussel-inspired polydopamine/polyethyleneimine (PDA/PEI) coating is employed to pattern enzyme-MOF in microfluidic channels and exploit the wettability gradient for {"}pumpless transportation{"} effect. As a proof of concept, we combine a cascade reaction of glucose oxidase (GOx) and horseradish peroxidase (HRP) enzymes to detect glucose into a patterned zeolitic imidazole framework-8 (ZIF-8) thin film on a flexible polymeric substrate. The results show that the ZIF-8/GOx&HRP in situ composites on PDA/PEI patterns have good acid and thermal stability compared with samples without ZIF-8. ZIF-8/GOx&HRP in situ shows high selectivity toward glucose, linear sensitivity of 0.00303 Abs/μM, and the limit of detection of 8 μM glucose concentration. An unexpected benefit of this approach is the ability of the ZIF-8 thin-film structure to provide a diffusion limiting effect for substrate influx, thus, producing high range of linear response range (8 μM to 5 mM of glucose). This work provides insights into the spatial location of the enzymes in MOF thin films and the potential of such patterning techniques for MOF-based biosensors using other types of biological elements such as antibodies and aptamers.",
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Metal-organic-framework-based enzymatic microfluidic biosensor via surface patterning and biomineralization. / Mohammad, Munirah; Razmjou, Amir; Liang, Kang; Asadnia, Mohsen; Chen, Vicki.

In: ACS Applied Materials and Interfaces, Vol. 11, No. 2, 2019, p. 1807-1820.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Metal-organic-framework-based enzymatic microfluidic biosensor via surface patterning and biomineralization

AU - Mohammad, Munirah

AU - Razmjou, Amir

AU - Liang, Kang

AU - Asadnia, Mohsen

AU - Chen, Vicki

PY - 2019

Y1 - 2019

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AB - Recently, the biomineralization of enzyme in metal-organic-framework (enzyme-MOF) composite have shown a great potential to increase enzymes stability without compromising their activity; hence, it is desirable for its applications in biosensing devices. Nonetheless, most of the enzyme-MOF research has been focusing on enzyme encapsulation in particle form, which limits its synthesis flexibility for practical applications because of its requirement for postsynthesis immobilization onto solid support. Therefore, to develop a diagnostic device out of the biomineralized enzyme, surface patterning and integration of microfluidic system offers many advantages. In this work, mussel-inspired polydopamine/polyethyleneimine (PDA/PEI) coating is employed to pattern enzyme-MOF in microfluidic channels and exploit the wettability gradient for "pumpless transportation" effect. As a proof of concept, we combine a cascade reaction of glucose oxidase (GOx) and horseradish peroxidase (HRP) enzymes to detect glucose into a patterned zeolitic imidazole framework-8 (ZIF-8) thin film on a flexible polymeric substrate. The results show that the ZIF-8/GOx&HRP in situ composites on PDA/PEI patterns have good acid and thermal stability compared with samples without ZIF-8. ZIF-8/GOx&HRP in situ shows high selectivity toward glucose, linear sensitivity of 0.00303 Abs/μM, and the limit of detection of 8 μM glucose concentration. An unexpected benefit of this approach is the ability of the ZIF-8 thin-film structure to provide a diffusion limiting effect for substrate influx, thus, producing high range of linear response range (8 μM to 5 mM of glucose). This work provides insights into the spatial location of the enzymes in MOF thin films and the potential of such patterning techniques for MOF-based biosensors using other types of biological elements such as antibodies and aptamers.

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KW - biosensors

KW - metal-organic framework

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KW - enzymes

KW - polydopamine

KW - pumpless transportation

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