Metal particle-enhanced fluorescent immunoassays on metal mirrors

Evgenia G. Matveeva, Ignacy Gryczynski, Anne Barnett, Zoya Leonenko, Joseph R. Lakowicz, Zygmunt Gryczynski

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

We present fluoroimmunoassays on plain metal-coated surfaces (metal mirrors) enhanced by metal nanoparticles (silver island films [SIFs]). Metal mirrors (aluminum, gold, or silver protected with a thin silica layer) were coated with SIFs, and an immunoassay (model assay for rabbit immunoglobulin G or myoglobin immunoassay) was performed on this surface using fluorescently labeled antibodies. Our results showed that SIFs alone (on glass surface not coated with metal) enhance the immunoassay signal approximately 3- to 10-fold. Using a metal mirror instead of glass as support for SIFs results in up to 50-fold signal enhancement.
Original languageEnglish
Pages (from-to)239-245
Number of pages7
JournalAnalytical Biochemistry
Volume363
Issue number2
DOIs
Publication statusPublished - 2007

Keywords

  • fluorescence immunoassay
  • metal-enhanced fluorescence
  • plasmonics
  • nano-size metal particles
  • silver island films

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