Metal particle-enhanced fluorescent immunoassays on metal mirrors

Evgenia G. Matveeva; Ignacy Gryczynski; Anne Barnett; Zoya Leonenko; Joseph R. Lakowicz; Zygmunt Gryczynski

doi:10.1016/j.ab.2007.01.030

Metal particle-enhanced fluorescent immunoassays on metal mirrors

Evgenia G. Matveeva, Ignacy Gryczynski, Anne Barnett, Zoya Leonenko, Joseph R. Lakowicz, Zygmunt Gryczynski

Research output: Contribution to journal › Article › peer-review

85 Citations (Scopus)

Abstract

We present fluoroimmunoassays on plain metal-coated surfaces (metal mirrors) enhanced by metal nanoparticles (silver island films [SIFs]). Metal mirrors (aluminum, gold, or silver protected with a thin silica layer) were coated with SIFs, and an immunoassay (model assay for rabbit immunoglobulin G or myoglobin immunoassay) was performed on this surface using fluorescently labeled antibodies. Our results showed that SIFs alone (on glass surface not coated with metal) enhance the immunoassay signal approximately 3- to 10-fold. Using a metal mirror instead of glass as support for SIFs results in up to 50-fold signal enhancement.

Original language	English
Pages (from-to)	239-245
Number of pages	7
Journal	Analytical Biochemistry
Volume	363
Issue number	2
DOIs	https://doi.org/10.1016/j.ab.2007.01.030
Publication status	Published - 2007

Keywords

fluorescence immunoassay
metal-enhanced fluorescence
plasmonics
nano-size metal particles
silver island films

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10.1016/j.ab.2007.01.030

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title = "Metal particle-enhanced fluorescent immunoassays on metal mirrors",

abstract = "We present fluoroimmunoassays on plain metal-coated surfaces (metal mirrors) enhanced by metal nanoparticles (silver island films [SIFs]). Metal mirrors (aluminum, gold, or silver protected with a thin silica layer) were coated with SIFs, and an immunoassay (model assay for rabbit immunoglobulin G or myoglobin immunoassay) was performed on this surface using fluorescently labeled antibodies. Our results showed that SIFs alone (on glass surface not coated with metal) enhance the immunoassay signal approximately 3- to 10-fold. Using a metal mirror instead of glass as support for SIFs results in up to 50-fold signal enhancement.",

keywords = "fluorescence immunoassay, metal-enhanced fluorescence, plasmonics, nano-size metal particles, silver island films",

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T1 - Metal particle-enhanced fluorescent immunoassays on metal mirrors

AU - Matveeva, Evgenia G.

AU - Gryczynski, Ignacy

AU - Barnett, Anne

AU - Leonenko, Zoya

AU - Lakowicz, Joseph R.

AU - Gryczynski, Zygmunt

PY - 2007

Y1 - 2007

N2 - We present fluoroimmunoassays on plain metal-coated surfaces (metal mirrors) enhanced by metal nanoparticles (silver island films [SIFs]). Metal mirrors (aluminum, gold, or silver protected with a thin silica layer) were coated with SIFs, and an immunoassay (model assay for rabbit immunoglobulin G or myoglobin immunoassay) was performed on this surface using fluorescently labeled antibodies. Our results showed that SIFs alone (on glass surface not coated with metal) enhance the immunoassay signal approximately 3- to 10-fold. Using a metal mirror instead of glass as support for SIFs results in up to 50-fold signal enhancement.

AB - We present fluoroimmunoassays on plain metal-coated surfaces (metal mirrors) enhanced by metal nanoparticles (silver island films [SIFs]). Metal mirrors (aluminum, gold, or silver protected with a thin silica layer) were coated with SIFs, and an immunoassay (model assay for rabbit immunoglobulin G or myoglobin immunoassay) was performed on this surface using fluorescently labeled antibodies. Our results showed that SIFs alone (on glass surface not coated with metal) enhance the immunoassay signal approximately 3- to 10-fold. Using a metal mirror instead of glass as support for SIFs results in up to 50-fold signal enhancement.

KW - fluorescence immunoassay

KW - metal-enhanced fluorescence

KW - plasmonics

KW - nano-size metal particles

KW - silver island films

UR - https://www.scopus.com/pages/publications/33947286758

U2 - 10.1016/j.ab.2007.01.030

DO - 10.1016/j.ab.2007.01.030

M3 - Article

C2 - 17316540

SN - 0003-2697

VL - 363

SP - 239

EP - 245

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Metal particle-enhanced fluorescent immunoassays on metal mirrors

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Keywords

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