TY - JOUR
T1 - Methicillin-resistant Staphylococcus sp. colonizing health care workers of a cancer hospital
AU - Costa, Dayane de Melo
AU - Kipnis, André
AU - Leão-Vasconcelos, Lara Stefânia Netto de Oliveira
AU - Rocha-Vilefort, Larissa Oliveira
AU - Telles, Sheila Araújo
AU - André, Maria Cláudia Dantas Porfírio Borges
AU - Tipple, Anaclara Ferreira Veiga
AU - Lima, Ana Beatriz Mori
AU - Ribeiro, Nádia Ferreira Gonçalves
AU - Pereira, Mayara Regina
AU - Prado-Palos, Marinésia Aparecida
N1 - Copyright the Sociedade Brasileira de Microbiologia 2014. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.
PY - 2014/7/1
Y1 - 2014/7/1
N2 - The aim of the study was to analyze epidemiological and microbiological aspects of oral colonization by methicillin-resistant Staphylococcus of health care workers in a cancer hospital. Interview and saliva sampling were performed with 149 health care workers. Antimicrobial resistance was determined by disk diffusion and minimum inhibitory concentration. Polymerase Chain Reaction, Internal Transcribed Spacer-Polymerase Chain Reaction and Pulsed Field Gel Electrophoresis were performed for genotypic characterization of methicillin-resistant Staphylococcus. Risk factors were determined by logistic regression. Methicillin-resistant Staphylococcus colonization prevalence was 19.5%, denture wearing (p = 0.03), habit of nail biting (p = 0.04) and preparation and administration of antimicrobial (p = 0.04) were risk factors identified. All methicillin-resistant Staphylococcus were S. epidermidis, 94.4% of them had mecA gene. Closely related and indistinguishable methicillinresistant S. epidermidis were detected. These results highlight that HCWs which have contact with patient at high risk for developing infections were identified as colonized by MRSE in the oral cavity, reinforcing this cavity as a reservoir of these bacteria and the risk to themselves and patients safety, because these microorganisms may be spread by coughing and talking.
AB - The aim of the study was to analyze epidemiological and microbiological aspects of oral colonization by methicillin-resistant Staphylococcus of health care workers in a cancer hospital. Interview and saliva sampling were performed with 149 health care workers. Antimicrobial resistance was determined by disk diffusion and minimum inhibitory concentration. Polymerase Chain Reaction, Internal Transcribed Spacer-Polymerase Chain Reaction and Pulsed Field Gel Electrophoresis were performed for genotypic characterization of methicillin-resistant Staphylococcus. Risk factors were determined by logistic regression. Methicillin-resistant Staphylococcus colonization prevalence was 19.5%, denture wearing (p = 0.03), habit of nail biting (p = 0.04) and preparation and administration of antimicrobial (p = 0.04) were risk factors identified. All methicillin-resistant Staphylococcus were S. epidermidis, 94.4% of them had mecA gene. Closely related and indistinguishable methicillinresistant S. epidermidis were detected. These results highlight that HCWs which have contact with patient at high risk for developing infections were identified as colonized by MRSE in the oral cavity, reinforcing this cavity as a reservoir of these bacteria and the risk to themselves and patients safety, because these microorganisms may be spread by coughing and talking.
KW - Health personnel
KW - Hospital
KW - Methicillin resistance
KW - Occupational health
KW - Oncology service
KW - Staphylococcus
UR - http://www.scopus.com/inward/record.url?scp=84909950200&partnerID=8YFLogxK
U2 - 10.1590/S1517-83822014000300006
DO - 10.1590/S1517-83822014000300006
M3 - Article
C2 - 25477910
AN - SCOPUS:84909950200
SN - 1517-8382
VL - 45
SP - 799
EP - 805
JO - Brazilian Journal of Microbiology
JF - Brazilian Journal of Microbiology
IS - 3
ER -