Method for monitoring hydrolytic activity

Peter Karuso (Inventor), Hung-yoon Choi (Inventor)

Research output: Patent

Abstract

The present invention relates to methods of measuring the activity of a hydrolytic agent comprising contacting a biomolecule with a hydrolytic agent in the presence of a fluorescent dye under conditions that allow digestion of the biomolecule by the hydrolytic agent. The fluorescence of the dye is monitored over time and a change in fluorescence signifies digestion of the biomolecule by the hydrolytic agent. The biomolecule is preferably a protein, peptide or proteome but can also be a carbohydrate, oligonucleotide or lipid. Further methods relate to determining an end point for digestion of a biomolecule by a hydrolytic agent, and methods of monitoring digestion of a biomolecule by a hydrolytic agent. The monitoring can be performed on the reaction mixture in real time or via sampling. The invention also relates to kits for carrying out the method
Original languageEnglish
Patent numberWO2007051257
IPCG01N 33/52
Priority date4/11/05
Publication statusPublished - 10 May 2007

Bibliographical note

from PCT/AU2006/001650

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