Method for monitoring hydrolytic activity

Peter Karuso (Inventor), Hung-yoon Choi (Inventor)

Research output: PatentResearch

Abstract

The present invention relates to methods of measuring the activity of a hydrolytic agent comprising contacting a biomolecule with a hydrolytic agent in the presence of a fluorescent dye under conditions that allow digestion of the biomolecule by the hydrolytic agent. The fluorescence of the dye is monitored over time and a change in fluorescence signifies digestion of the biomolecule by the hydrolytic agent. The biomolecule is preferably a protein, peptide or proteome but can also be a carbohydrate, oligonucleotide or lipid. Further methods relate to determining an end point for digestion of a biomolecule by a hydrolytic agent, and methods of monitoring digestion of a biomolecule by a hydrolytic agent. The monitoring can be performed on the reaction mixture in real time or via sampling. The invention also relates to kits for carrying out the method
LanguageEnglish
Patent numberWO2007/051257
IPCG01N 33/52
Priority date4/11/05
Publication statusPublished - 10 May 2007

Fingerprint

Biomolecules
Monitoring
Patents and inventions
Fluorescence
Proteome
Fluorescent Dyes
Oligonucleotides
Coloring Agents
Carbohydrates
Sampling
Lipids
Peptides
Proteins

Bibliographical note

from PCT/AU2006/001650

Cite this

Karuso, P., & Choi, H. (2007). IPC No. G01N 33/52. Method for monitoring hydrolytic activity. (Patent No. WO2007/051257).
Karuso, Peter (Inventor) ; Choi, Hung-yoon (Inventor). / Method for monitoring hydrolytic activity. IPC No.: G01N 33/52. Patent No.: WO2007/051257.
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abstract = "The present invention relates to methods of measuring the activity of a hydrolytic agent comprising contacting a biomolecule with a hydrolytic agent in the presence of a fluorescent dye under conditions that allow digestion of the biomolecule by the hydrolytic agent. The fluorescence of the dye is monitored over time and a change in fluorescence signifies digestion of the biomolecule by the hydrolytic agent. The biomolecule is preferably a protein, peptide or proteome but can also be a carbohydrate, oligonucleotide or lipid. Further methods relate to determining an end point for digestion of a biomolecule by a hydrolytic agent, and methods of monitoring digestion of a biomolecule by a hydrolytic agent. The monitoring can be performed on the reaction mixture in real time or via sampling. The invention also relates to kits for carrying out the method",
author = "Peter Karuso and Hung-yoon Choi",
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Karuso, P & Choi, H 2007, Method for monitoring hydrolytic activity, Patent No. WO2007/051257, IPC No. G01N 33/52.

Method for monitoring hydrolytic activity. / Karuso, Peter (Inventor); Choi, Hung-yoon (Inventor).

IPC No.: G01N 33/52. Patent No.: WO2007/051257.

Research output: PatentResearch

TY - PAT

T1 - Method for monitoring hydrolytic activity

AU - Karuso, Peter

AU - Choi, Hung-yoon

N1 - from PCT/AU2006/001650

PY - 2007/5/10

Y1 - 2007/5/10

N2 - The present invention relates to methods of measuring the activity of a hydrolytic agent comprising contacting a biomolecule with a hydrolytic agent in the presence of a fluorescent dye under conditions that allow digestion of the biomolecule by the hydrolytic agent. The fluorescence of the dye is monitored over time and a change in fluorescence signifies digestion of the biomolecule by the hydrolytic agent. The biomolecule is preferably a protein, peptide or proteome but can also be a carbohydrate, oligonucleotide or lipid. Further methods relate to determining an end point for digestion of a biomolecule by a hydrolytic agent, and methods of monitoring digestion of a biomolecule by a hydrolytic agent. The monitoring can be performed on the reaction mixture in real time or via sampling. The invention also relates to kits for carrying out the method

AB - The present invention relates to methods of measuring the activity of a hydrolytic agent comprising contacting a biomolecule with a hydrolytic agent in the presence of a fluorescent dye under conditions that allow digestion of the biomolecule by the hydrolytic agent. The fluorescence of the dye is monitored over time and a change in fluorescence signifies digestion of the biomolecule by the hydrolytic agent. The biomolecule is preferably a protein, peptide or proteome but can also be a carbohydrate, oligonucleotide or lipid. Further methods relate to determining an end point for digestion of a biomolecule by a hydrolytic agent, and methods of monitoring digestion of a biomolecule by a hydrolytic agent. The monitoring can be performed on the reaction mixture in real time or via sampling. The invention also relates to kits for carrying out the method

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M3 - Patent

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Karuso P, Choi H, inventors. Method for monitoring hydrolytic activity. G01N 33/52. 2007 May 10.