Mhp107 is a member of the multifunctional adhesin family of Mycoplasma hyopneumoniae

Lisa M. Seymour, Linda Falconer, Ania T. Deutscher, F. Chris Minion, Matthew P. Padula, Nicholas E. Dixon, Steven P. Djordjevic, Mark J. Walker*

*Corresponding author for this work

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Mycoplasma hyopneumoniae is the causative pathogen of porcine enzootic pneumonia, an economically significant disease that disrupts the mucociliary escalator in the swine respiratory tract. Expression of Mhp107, a P97 paralog encoded by the gene mhp107, was confirmed using ESI-MS/MS. To investigate the function of Mhp107, three recombinant proteins, F1(Mhp107), F2(Mhp107), and F3(Mhp107), spanning the N-terminal, central, and C-terminal regions of Mhp107 were constructed. Colonization of swine by M. hyopneumoniae requires adherence of the bacterium to ciliated cells of the respiratory tract. Recent studies have identified a number of M. hyopneumoniae adhesins that bind heparin, fibronectin, and plasminogen. F1(Mhp107) was found to bind porcine heparin (K(D) similar to 90 nM) in a dose-dependent and saturable manner, whereas F3(Mhp107) bound fibronectin (K(D) similar to 180 nM) at physiologically relevant concentrations. F1(Mhp107) also bound porcine plasminogen (K(D) = 24 nM) in a dose-dependent and physiologically relevant manner. Microspheres coated with F3(Mhp107) mediate adherence to porcine kidney epithelial-like (PK15) cells, and all three recombinant proteins (F1(Mhp107)-F3(Mhp107)) bound swine respiratory cilia. Together, these findings indicate that Mhp107 is a member of the multifunctional M. hyopneumoniae adhesin family of surface proteins and contributes to both adherence to the host and pathogenesis.

Original languageEnglish
Pages (from-to)10097-10104
Number of pages8
JournalJournal of Biological Chemistry
Volume286
Issue number12
DOIs
Publication statusPublished - 25 Mar 2011
Externally publishedYes

Keywords

  • Fibronectin
  • Heparin Binding protein
  • Multifunctional Protein
  • Plasminogen
  • Surface Plasmon Resonance (SPR)
  • Mycoplasma Hyopneumoniae
  • Adhesin

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