Microdetermination of nucleotides in hydrolyzates of RNA

J. M. Gebicki*, Simon Freed

*Corresponding author for this work

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Lithium hydroxide, rather than the usual potassium hydroxide, was used to hydrolyze microgram amounts of RNA. After neutralization, lithium perchlorate was removed by extraction with isopropanol-ether; this eliminated the need for desalting of the mixture of nucleotides obtained, which were separated by thin-layer chromatography and measured with a precision of ±5%.

Original languageEnglish
Pages (from-to)253-257
Number of pages5
JournalAnalytical Biochemistry
Volume14
Issue number2
Publication statusPublished - Feb 1966
Externally publishedYes

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