Abstract
Lithium hydroxide, rather than the usual potassium hydroxide, was used to hydrolyze microgram amounts of RNA. After neutralization, lithium perchlorate was removed by extraction with isopropanol-ether; this eliminated the need for desalting of the mixture of nucleotides obtained, which were separated by thin-layer chromatography and measured with a precision of ±5%.
| Original language | English |
|---|---|
| Pages (from-to) | 253-257 |
| Number of pages | 5 |
| Journal | Analytical Biochemistry |
| Volume | 14 |
| Issue number | 2 |
| Publication status | Published - Feb 1966 |
| Externally published | Yes |