Microdetermination of nucleotides in hydrolyzates of RNA

J. M. Gebicki; Simon Freed

Microdetermination of nucleotides in hydrolyzates of RNA

J. M. Gebicki^*, Simon Freed

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Lithium hydroxide, rather than the usual potassium hydroxide, was used to hydrolyze microgram amounts of RNA. After neutralization, lithium perchlorate was removed by extraction with isopropanol-ether; this eliminated the need for desalting of the mixture of nucleotides obtained, which were separated by thin-layer chromatography and measured with a precision of ±5%.

Original language	English
Pages (from-to)	253-257
Number of pages	5
Journal	Analytical Biochemistry
Volume	14
Issue number	2
Publication status	Published - Feb 1966
Externally published	Yes

Cite this

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title = "Microdetermination of nucleotides in hydrolyzates of RNA",

abstract = "Lithium hydroxide, rather than the usual potassium hydroxide, was used to hydrolyze microgram amounts of RNA. After neutralization, lithium perchlorate was removed by extraction with isopropanol-ether; this eliminated the need for desalting of the mixture of nucleotides obtained, which were separated by thin-layer chromatography and measured with a precision of ±5\%.",

author = "Gebicki, \{J. M.\} and Simon Freed",

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T1 - Microdetermination of nucleotides in hydrolyzates of RNA

AU - Gebicki, J. M.

AU - Freed, Simon

PY - 1966/2

Y1 - 1966/2

N2 - Lithium hydroxide, rather than the usual potassium hydroxide, was used to hydrolyze microgram amounts of RNA. After neutralization, lithium perchlorate was removed by extraction with isopropanol-ether; this eliminated the need for desalting of the mixture of nucleotides obtained, which were separated by thin-layer chromatography and measured with a precision of ±5%.

AB - Lithium hydroxide, rather than the usual potassium hydroxide, was used to hydrolyze microgram amounts of RNA. After neutralization, lithium perchlorate was removed by extraction with isopropanol-ether; this eliminated the need for desalting of the mixture of nucleotides obtained, which were separated by thin-layer chromatography and measured with a precision of ±5%.

UR - https://www.scopus.com/pages/publications/0013878293

M3 - Article

C2 - 5939861

AN - SCOPUS:0013878293

SN - 0003-2697

VL - 14

SP - 253

EP - 257

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Microdetermination of nucleotides in hydrolyzates of RNA

Abstract

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