Microfluidic capture of endothelial progenitor cells in human blood samples

J. J. Li, D. Broqueres-You, Z. T. Han, W. T. He, S. S. Li, L. M. Jiang, B. I. Levy, J. Shi*, Y. Chen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

We present a method to efficiently trap circulating endothelial progenitor cells (EPCs) in human blood samples by using a microfluidic device with a short cell traveling length. This device consists of a wide channel separated into three parallel parts by two linear arrays of micro-columns. Flow-derivation microstructures are patterned on both up- and down-side walls of the center part of the channel, forcing the cells to encounter the narrowly spaced micro-columns and then be trapped with the help of specified immunological interaction. Both theoretical and experimental data confirmed the reliability of this concept. By using human umbilical cord blood-derived EPCs spiked into a buffer solution or a blood sample, we demonstrate a capture rate of more than 50% for a traveling length of only 1 cm, which is clearly advantageous in terms of cell trapping efficiency and image processing speed.

Original languageEnglish
Pages (from-to)262-266
Number of pages5
JournalMicroelectronic Engineering
Volume111
DOIs
Publication statusPublished - 2013
Externally publishedYes

Keywords

  • Endothelial progenitor cells
  • Microdevices
  • Rare cell trapping

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