Microfluidic data acusition for digital quantification using amplified single molecule detection

Jonas Melin, Jonas Jarvius, Jenny G̈ransson, Johan Stenberg, Fredrik Nikolajeff, Mats Nilsson*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contribution

Abstract

Amplified single-molecule detection constitutes a scheme for biomolecule enumeration by transforming specific molecular recognition events at nanometer dimensions to optically detectable micrometer-sized DNA macromolecules. Unlike traditional ensemble average measurements, our detection approach preserves the discrete nature of the molecular population. At flow rates higher than 3 uμl/min confocal data acquisition results in a precision limited by Poisson counting statistics.

Original languageEnglish
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherJapan Academic Association Inc
Pages723-725
Number of pages3
Publication statusPublished - 2006
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
Duration: 5 Nov 20069 Nov 2006

Other

Other10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
CountryJapan
CityTokyo
Period5/11/069/11/06

Keywords

  • Confocal detection
  • Single molecule detection

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