Microfluidic data acusition for digital quantification using amplified single molecule detection

Jonas Melin, Jonas Jarvius, Jenny G̈ransson, Johan Stenberg, Fredrik Nikolajeff, Mats Nilsson*

*Corresponding author for this work

    Research output: Chapter in Book/Report/Conference proceedingConference proceeding contributionpeer-review

    Abstract

    Amplified single-molecule detection constitutes a scheme for biomolecule enumeration by transforming specific molecular recognition events at nanometer dimensions to optically detectable micrometer-sized DNA macromolecules. Unlike traditional ensemble average measurements, our detection approach preserves the discrete nature of the molecular population. At flow rates higher than 3 uμl/min confocal data acquisition results in a precision limited by Poisson counting statistics.

    Original languageEnglish
    Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    PublisherJapan Academic Association Inc
    Pages723-725
    Number of pages3
    Publication statusPublished - 2006
    Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
    Duration: 5 Nov 20069 Nov 2006

    Other

    Other10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
    Country/TerritoryJapan
    CityTokyo
    Period5/11/069/11/06

    Keywords

    • Confocal detection
    • Single molecule detection

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