Microfluidic devices with disposable enzyme electrode for electrochemical monitoring of glucose concentrations

Xin Li, Fan Zhang, Jian Shi, Li Wang, Jing Hua Tian, Xiong Tu Zhou, Lian Mei Jiang, Li Liu, Zhen Jie Zhao, Pin Gang He, Yong Chen*

*Corresponding author for this work

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

This article describes the fabrication of tube-like microchannels made of UV curable polymer on a glass substrate and the device assembling with a disposable enzyme-working electrode for high-sensitivity electrochemical detection. While both reference and counter electrodes are patterned on the surface of the glass substrate, the working electrode is flipped on the top of the channel with an open access, providing a face-to-face probing configuration. When the enzyme electrode is contaminated or degraded, it can be easily replaced by a new one, keeping the main body of the device and the detection schema unchanged. Using glucose oxidase-coated gold electrodes, we were able to determine a linear amperometry response to the glucose concentrations in the range of 2-16mM. By replacing the as-prepared working electrode by the one after thermal treatments, we showed a much more degraded enzyme electrode activity, enabling efficient determination of the electrode quality as well as the whole process optimization.

Original languageEnglish
Pages (from-to)3201-3206
Number of pages6
JournalElectrophoresis
Volume32
Issue number22
DOIs
Publication statusPublished - Nov 2011
Externally publishedYes

Keywords

  • Electrochemical detection
  • Microdevices
  • Microfluidics

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