@inbook{163cbe2551c3414ab31a1c4deaa0f0eb,
title = "Microinjection of zygotes for CRISPR/Cas9-mediated insertion of transgenes into the murine Rosa26 safe harbor",
abstract = "Genetically modified (GM) mice are widely used in biomedical research because they can address complex questions in an in-vivo setting that could not otherwise be addressed in-vitro. Microinjection of zygotes remains the most common technique to generate GM animals to date. Here, we describe the targeted insertion (knock-in) of transgenes by microinjection of 1-cell or 2-cell stage embryos into the murine Rosa26 safe harbor.",
keywords = "Genotyping, Microinjection, Pronuclei, Reimplantation, Rosa26, Transgene, Zygotes",
author = "Fabien Delerue and Ittner, {Lars M.}",
year = "2022",
doi = "10.1007/978-1-0716-2301-5_7",
language = "English",
isbn = "9781071623008",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "115--128",
editor = "Verma, {Paul John} and Huseyin Sumer and Jun Liu",
booktitle = "Applications of genome modulation and editing",
}