Microiontophoretic study of individual neurons during intracellular recording

Qi Jian Sun*, Paul M. Pilowsky

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

There is an increasing tendency to combine multidisciplinary methods in studying the central nervous systems. One recently developed technique is the combination of electrophysiological and pharmacological investigations by making intracellular recording during extracellular iontophoresis of drugs to study the effects of neurotransmitters on neuronal activity and connections. In this chapter, we describe this technique in detail. We show how: 1. Electrodes are made for this type of study. 2. Drug solutions are prepared for current iontophoresis. 3. A microiontophoresis current generator is used to iontophoretically inject drugs onto a neuron while performing intracellular recording. At the end of this chapter, we provide an example from our recent studies that uses this iontophoretic technique to study effect of counter-intuitive inhibitory effect of blocking GABAA receptors in the control of post-inspiratory activity of the expiratory laryngeal motoneurons.

Original languageEnglish
Title of host publicationStimulation and Inhibition of Neurons
EditorsPaul M. Pilowsky, Melissa M.J. Farnham, Angelina Y. Fong
Place of PublicationNew York
PublisherHumana Press
Pages141-149
Number of pages9
ISBN (Electronic)9781627032339
ISBN (Print)9781627032322, 1627032320
DOIs
Publication statusPublished - 2013

Publication series

NameNeuromethods
PublisherHumana Press
Volume78
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

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