TY - JOUR
T1 - Microplate reader-based quantitation of collagens
AU - Walsh, Bradley J.
AU - Thornton, Stephen C.
AU - Penny, Ronald
AU - Breit, Samuel N.
PY - 1992
Y1 - 1992
N2 - By using a picrosirius dye, sensitive and specific staining of collagens plated in microtiter wells was achieved. The range of detection was from 0.5 to 20 μg. Human collagen types I, III, IV, and V were tested and able to be detected by the method. The dye did not bind to acetylcholinesterase or elastin. It did bind to C1q to some extent but this is not surprising since the molecule contains some triple helical collagen-like structures. A comparison performed between this assay and a colorimetric assay for hydroxyproline using tissue culture supernatants gave similar results for both samples. Due to its simplicity and sensitivity this assay will be most useful in laboratories where large numbers of samples must be screened for collagen production.
AB - By using a picrosirius dye, sensitive and specific staining of collagens plated in microtiter wells was achieved. The range of detection was from 0.5 to 20 μg. Human collagen types I, III, IV, and V were tested and able to be detected by the method. The dye did not bind to acetylcholinesterase or elastin. It did bind to C1q to some extent but this is not surprising since the molecule contains some triple helical collagen-like structures. A comparison performed between this assay and a colorimetric assay for hydroxyproline using tissue culture supernatants gave similar results for both samples. Due to its simplicity and sensitivity this assay will be most useful in laboratories where large numbers of samples must be screened for collagen production.
UR - http://www.scopus.com/inward/record.url?scp=0026747051&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(92)90301-M
DO - 10.1016/0003-2697(92)90301-M
M3 - Article
C2 - 1384382
AN - SCOPUS:0026747051
SN - 0003-2697
VL - 203
SP - 187
EP - 190
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -