Microplate reader-based quantitation of collagens

Bradley J. Walsh*, Stephen C. Thornton, Ronald Penny, Samuel N. Breit

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

95 Citations (Scopus)

Abstract

By using a picrosirius dye, sensitive and specific staining of collagens plated in microtiter wells was achieved. The range of detection was from 0.5 to 20 μg. Human collagen types I, III, IV, and V were tested and able to be detected by the method. The dye did not bind to acetylcholinesterase or elastin. It did bind to C1q to some extent but this is not surprising since the molecule contains some triple helical collagen-like structures. A comparison performed between this assay and a colorimetric assay for hydroxyproline using tissue culture supernatants gave similar results for both samples. Due to its simplicity and sensitivity this assay will be most useful in laboratories where large numbers of samples must be screened for collagen production.

Original languageEnglish
Pages (from-to)187-190
Number of pages4
JournalAnalytical Biochemistry
Volume203
Issue number2
DOIs
Publication statusPublished - 1992
Externally publishedYes

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