Minimal information for studies of extracellular vesicles (MISEV2023): from basic to advanced approaches

Joshua A. Welsh; Deborah C. I. Goberdhan; Lorraine O’Driscoll; Edit I. Buzas; Cherie Blenkiron; Benedetta Bussolati; Houjian Cai; Dolores Di Vizio; Tom A.P. Driedonks; Uta Erdbrügger; Juan M. Falcon-Perez; Qing Ling Fu; Andrew F. Hill; Metka Lenassi; Sai Kiang Lim; Mỹ G. Mahoney; Sujata Mohanty; Andreas Möller; Rienk Nieuwland; Takahiro Ochiya; Susmita Sahoo; Ana C. Torrecilhas; Lei Zheng; Andries Zijlstra; Sarah Abuelreich; Reem Bagabas; Paolo Bergese; Esther M. Bridges; Marco Brucale; Dylan Burger; Randy P. Carney; Emanuele Cocucci; Rossella Crescitelli; Edveena Hanser; Adrian L. Harris; Norman J. Haughey; An Hendrix; Alexander R. Ivanov; Tijana Jovanovic-Talisman; Nicole A. Kruh-Garcia; Vroniqa Ku’ulei Lyn Faustino; Diego Kyburz; Cecilia Lässer; Kathleen M. Lennon; Jan Lötvall; Adam L. Maddox; Elena S. Martens-Uzunova; Rachel R. Mizenko; Lauren A. Newman; Andrea Ridolfi; Eva Rohde; Tatu Rojalin; Andrew Rowland; Andras Saftics; Ursula S. Sandau; Julie A. Saugstad; Faezeh Shekari; Simon Swift; Dmitry Ter-Ovanesyan; Juan P. Tosar; Zivile Useckaite; Francesco Valle; Zoltan Varga; Edwin van der Pol; Martijn J. C. van Herwijnen; Marca H. M. Wauben; Ann M. Wehman; Sarah Williams; Andrea Zendrini; Alan J. Zimmerman; MISEV Consortium; Guoping Li; Kenneth W. Witwer; Clotilde Thery

doi:10.1002/jev2.12404

Minimal information for studies of extracellular vesicles (MISEV2023): from basic to advanced approaches

Joshua A. Welsh^*, Deborah C. I. Goberdhan^*, Lorraine O’Driscoll^*, Edit I. Buzas, Cherie Blenkiron, Benedetta Bussolati, Houjian Cai, Dolores Di Vizio, Tom A.P. Driedonks, Uta Erdbrügger, Juan M. Falcon-Perez, Qing Ling Fu, Andrew F. Hill, Metka Lenassi, Sai Kiang Lim, Mỹ G. Mahoney, Sujata Mohanty, Andreas Möller, Rienk Nieuwland, Takahiro OchiyaSusmita Sahoo, Ana C. Torrecilhas, Lei Zheng, Andries Zijlstra, Sarah Abuelreich, Reem Bagabas, Paolo Bergese, Esther M. Bridges, Marco Brucale, Dylan Burger, Randy P. Carney, Emanuele Cocucci, Rossella Crescitelli, Edveena Hanser, Adrian L. Harris, Norman J. Haughey, An Hendrix, Alexander R. Ivanov, Tijana Jovanovic-Talisman, Nicole A. Kruh-Garcia, Vroniqa Ku’ulei Lyn Faustino, Diego Kyburz, Cecilia Lässer, Kathleen M. Lennon, Jan Lötvall, Adam L. Maddox, Elena S. Martens-Uzunova, Rachel R. Mizenko, Lauren A. Newman, Andrea Ridolfi, Eva Rohde, Tatu Rojalin, Andrew Rowland, Andras Saftics, Ursula S. Sandau, Julie A. Saugstad, Faezeh Shekari, Simon Swift, Dmitry Ter-Ovanesyan, Juan P. Tosar, Zivile Useckaite, Francesco Valle, Zoltan Varga, Edwin van der Pol, Martijn J. C. van Herwijnen, Marca H. M. Wauben, Ann M. Wehman, Sarah Williams, Andrea Zendrini, Alan J. Zimmerman, MISEV Consortium, Guoping Li, Kenneth W. Witwer, Clotilde Thery

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.

Original language	English
Article number	e12404
Pages (from-to)	1-84
Number of pages	84
Journal	Journal of Extracellular Vesicles
Volume	13
Issue number	2
DOIs	https://doi.org/10.1002/jev2.12404
Publication status	Published - Feb 2024

Bibliographical note

Copyright the Author(s) 2024. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

A correction exists for this article, the current version has been updated. The correction can be found at doi: 10.1002/jev2.12451

Keywords

ectosomes
exosomes
extracellular particles
extracellular vesicles
guidelines
microparticles
microvesicles
minimal information requirements
MISEV
reproducibility
rigor
standardisation

Access to Document

10.1002/jev2.12404Licence: CC BY

Publisher version (open access)Final published version, 2.46 MBLicence: CC BY

Cite this

Welsh, J. A., Goberdhan, D. C. I., O’Driscoll, L., Buzas, E. I., Blenkiron, C., Bussolati, B., Cai, H., Di Vizio, D., Driedonks, T. A. P., Erdbrügger, U., Falcon-Perez, J. M., Fu, Q. L., Hill, A. F., Lenassi, M., Lim, S. K., Mahoney, M. G., Mohanty, S., Möller, A., Nieuwland, R., ... Thery, C. (2024). Minimal information for studies of extracellular vesicles (MISEV2023): from basic to advanced approaches. Journal of Extracellular Vesicles, 13(2), 1-84. Article e12404. https://doi.org/10.1002/jev2.12404

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title = "Minimal information for studies of extracellular vesicles (MISEV2023): from basic to advanced approaches",

abstract = "Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its {\textquoteleft}Minimal Information for Studies of Extracellular Vesicles{\textquoteright}, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.",

keywords = "ectosomes, exosomes, extracellular particles, extracellular vesicles, guidelines, microparticles, microvesicles, minimal information requirements, MISEV, reproducibility, rigor, standardisation",

author = "Welsh, {Joshua A.} and Goberdhan, {Deborah C. I.} and Lorraine O{\textquoteright}Driscoll and Buzas, {Edit I.} and Cherie Blenkiron and Benedetta Bussolati and Houjian Cai and {Di Vizio}, Dolores and Driedonks, {Tom A.P.} and Uta Erdbr{\"u}gger and Falcon-Perez, {Juan M.} and Fu, {Qing Ling} and Hill, {Andrew F.} and Metka Lenassi and Lim, {Sai Kiang} and Mahoney, {Mỹ G.} and Sujata Mohanty and Andreas M{\"o}ller and Rienk Nieuwland and Takahiro Ochiya and Susmita Sahoo and Torrecilhas, {Ana C.} and Lei Zheng and Andries Zijlstra and Sarah Abuelreich and Reem Bagabas and Paolo Bergese and Bridges, {Esther M.} and Marco Brucale and Dylan Burger and Carney, {Randy P.} and Emanuele Cocucci and Rossella Crescitelli and Edveena Hanser and Harris, {Adrian L.} and Haughey, {Norman J.} and An Hendrix and Ivanov, {Alexander R.} and Tijana Jovanovic-Talisman and Kruh-Garcia, {Nicole A.} and Faustino, {Vroniqa Ku{\textquoteright}ulei Lyn} and Diego Kyburz and Cecilia L{\"a}sser and Lennon, {Kathleen M.} and Jan L{\"o}tvall and Maddox, {Adam L.} and Martens-Uzunova, {Elena S.} and Mizenko, {Rachel R.} and Newman, {Lauren A.} and Andrea Ridolfi and Eva Rohde and Tatu Rojalin and Andrew Rowland and Andras Saftics and Sandau, {Ursula S.} and Saugstad, {Julie A.} and Faezeh Shekari and Simon Swift and Dmitry Ter-Ovanesyan and Tosar, {Juan P.} and Zivile Useckaite and Francesco Valle and Zoltan Varga and {van der Pol}, Edwin and {van Herwijnen}, {Martijn J. C.} and Wauben, {Marca H. M.} and Wehman, {Ann M.} and Sarah Williams and Andrea Zendrini and Zimmerman, {Alan J.} and {MISEV Consortium} and Samar Ahmad and Ahmed, {Dina A. K.} and Ahmed, {Sarah H.} and Elena Aikawa and Naveed Akbar and Kazunari Akiyoshi and Al-Adra, {David P.} and Al-Masawa, {Maimonah E.} and Manuel Albanese and Ainhoa Alberro and Alcaraz, {Mar{\'i}a Jos{\'e}} and Jen Alexander-Brett and Alexander, {Kimberley L.} and Nilufar Ali and Alibhai, {Faisal J.} and Susann Allelein and Allenby, {Mark C.} and Fausto Almeida and {de Almeida}, {Luis Pereira} and Almousa, {Sameh W.} and Nihal Altan-Bonnet and Altei, {Wanessa F.} and Gloria Alvarez-Llamas and Alvarez, {Cora L.} and An, {Hyo Jung} and Krishnan Anand and Andaloussi, {Samir E. L.} and Anderson, {Johnathon D.} and Ramaroson Andriantsitohaina and Ansari, {Khairul I.} and Achille Anselmo and Anna Antoniou and Farrukh Aqil and Tanina Arab and Fabienne Archer and Syrine Arif and Armstrong, {David A.} and Arntz, {Onno J.} and Pierre Ars{\`e}ne and Luis Arteaga-Blanco and Nandini Asokan and Trude Aspelin and Atkin-Smith, {Georgia K.} and Dimitri Aubert and Ayyar, {Kanchana K.} and Maryam Azlan and Ioannis Azoidis and Ana{\"i}s B{\'e}cot and Bach, {Jean Marie} and Daniel Bachurski and Seoyoon Bae and Bagge, {Roger Olofsson} and Monika Baj-Krzyworzeka and Leonora Balaj and Carolina Balbi and {van Balkom}, {Bas W. M.} and Ballal, {Abhijna R.} and Afsareen Bano and S{\'e}bastien Banzet and Yonis Bare and Lucio Barile and Bahnisikha Barman and Isabel Barranco and Valeria Barreca and Genevi{\`e}ve Bart and Barteneva, {Natasha S.} and Manuela Basso and Mona Batish and Bauer, {Natalie R.} and Baxter, {Amy A.} and Bazi{\'e}, {Wilfried W.} and Erica Bazzan and Beaumont, {Joel E. J.} and Mary Bebawy and Bebelman, {Maarten P.} and Apolonija Bedina-Zavec and Beetler, {Danielle J.} and Tam{\'a}s Beke-Somfai and Cl{\'e}mence Belleann{\'e}e and Benedikter, {Birke J.} and Benediktsd{\'o}ttir, {Berglind E.} and Berardi, {Anna C.} and Mathilde Bergamelli and Irene Bertolini and Asima Bhattacharyya and Bhattacharyya, {Suvendra N.} and Biller, {Steven J.} and Clotilde Billottet and Bissler, {John J.} and Olivier Blanc-Brude and Blijdorp, {Charles J.} and Sylwia Bobis-Wozowicz and Victor Bodart-Santos and Bodn{\'a}r, {Bernadett R.} and Eric Boilard and Wilfrid Boireau and Vladimir Bokun and Bollard, {Stephanie M.} and Sveva Bollini and Antonella Bongiovanni and Laura Bongiovanni and Amandine Bonifay and Boppart, {Marni D.} and Francesce Borr{\`a}s and Steffi Bosch and Daniela Boselli and Massimo Bottini and Jeff Bouffard and Boulanger, {Chantal M.} and Boutros, {Paul C.} and Oscar Boyadjian and Boysen, {Anders T.} and Bozkurt, {Batuhan T.} and Bramich, {Kyle P.} and Fabian Braun and Bravo-Miana, {Roc{\'i}o Del Carmen} and Breakefield, {Xandra O.} and Santra Brenna and Kieran Brennan and Meadhbh Brennan and Koen Breyne and Brigstock, {David R.} and Brisson, {Alain R.} and Chaya Brodie and Katelyna Bruno and Cecilia Bucci and Shilpa Buch and Amyh Buck and M{\'a}ty{\'a}s Bukva and Bulte, {Jeff W. M.} and Sandra Buratta and Olivier Burgy and Burnier, {Julia V.} and Kaiping Burrows and Sara Busatto and Krisztina Buzas and Byrd, {J. Brian} and Albano C{\'a}ceres-Verschae and Caires, {Hugo R.} and Carmen Campos-Silva and Giovanni Camussi and {de Candia}, Paola and Carmen Carceller and Carmen Fernandez-Becerra and Carrasco, {Alexis G.Murillo} and Carter, {David R. F.} and Sara Cavallaro and Serena Cavallero and Sophie Cavallero and Crist{\'o}bal Cerda-Troncoso and Hao Chen and Qiang Chen and Shuai Chen and Siyu Chen and Yunxi Chen and Lesley Cheng and Chernyshev, {Vasiliy S.} and Chetty, {Venkatesh Kumar} and Chitti, {Sai V.} and Ssang-Goo Cho and Yoon-Kyoung Cho and Choi, {Byeong Hyeon} and Somchai Chutipongtanate and Cicardi, {Maria Elena} and Anna Cifuentes-Ruis and Alessandra Ciullo and Aled Clayton and Cleary, {Jacob A.} and Federico Cocozza and Emanuele Cocucci and Coffey, {Robert J.} and Federica Collino and Federico Colombo and Pascal Colosetti and Alvaro Compa{\~n}-Bertomeu and Julie Constanzo and Denis Corbeil and Anabela Cordeiro-da-Silva and J{\'u}lia Costa and Yvonne Couch and Yvan Courageux and Kelly Coutant and Beth Coyle and Rossella Crescitelli and Marina Cretich and Andr{\'e} Cronemberger-Andrade and Crossland, {Rachel E.} and Cucher, {Marcela A.} and Malgorzata Czystowska-Kuzmicz and Pasquale D'Acunzo and Igea D'Agnano and D'Agostino, {Vito G.} and Gisela D'Angelo and Daniele D'Arrigo and Crislyn D'Souza-Schorey and Dagur, {Raghubendra S.} and Danielson, {Kirsty M.} and Saumya Das and Thibaud Dauphin and Davidson, {Sean M.} and Davies, {Owen G.} and Davies, {Rebecca L.} and Davis, {Chelsea N.} and Gagan Deep and Jonathan Degosserie and {Van Delen}, Mats and Vatsal Deliwala and Dellar, {Elizabeth R.} and {Van Deun}, Jan and Apurba Dev and Sarah Deville and Andrew Devitt and Bert Dhondt and Dieterich, {Lothar C.} and Dittmer, {Dirk P.} and Brian Dobosh and Gabriella Dobra and Navneet Dogra and Eisuke Dohi and Vincenza Dolo and Domashevich, {Timothy V.} and Massimo Dominici and Liang Dong and Etienne Dor{\'e} and Dragovic, {Rebecca A.} and Driedonks, {Tom A. P.} and Lila Drittanti and Marvin Droste and Wei Duan and Esmahan Durmaz and Suman Dutta and Takanori Eguchi and Eichenberger, {Ramon M.} and Erez Eitan and Karin Ekstr{\"o}m and Maria Eldh and Celine Elie-Caille and Agustin Enciso-Martinez and Uta Erdbr{\"u}gger and Rezvan Esmaeili and Camille Ettelaie and F{\"o}rs{\"o}nits, {Andr{\'a}s I.} and Muller Fabbri and Marco Falasca and Falcon-Perez, {Juan M.} and Hongkuan Fan and Farah Fatima and Faustino, {Vroniqa Ku{\textquoteright}ulei-Lyn} and Alireza Fazeli and Mar{\'i}a Fern{\'a}ndez-Rhodes and Christopher Fernandez-Prada and Ferraro, {Mariola J.} and Ferreira, {Joao N.} and Ferreira, {Rafaela F.} and Figueroa-Hall, {Leandra K.} and Figueroa-Vald{\'e}s, {Aliosha I.} and Fioretti, {Paolo V.} and Sabine Flenady and Miguel Flores-Bellver and Fok, {Ellis K.} and Pamali Fonseka and Karen Forbes and Ford, {Verity J.} and Cristina Fornaguera and Dorian Forte and Stefano Forte and Orazio Fortunato and Franklin, {Jeffrey L.} and Daniela Freitas and Annie Frelet-Barrand and Qing-Ling Fu and Yu Fujita and Kathrin G{\"a}rtner and Andr{\'e} G{\"o}rgens and Gabriel, {{\'A}urea M.} and Martina Gabrielli and Susanne Gabrielsson and Alicia Galinsoga and Andrea Galisova and Gamage, {Teena K. J. B.} and Yingtang Gao and Marta Garcia-Contreras and Garcia, {M. Madhy Garcia} and Garcia, {Maria No{\'e}} and Ernesto Gargiulo and Garibotti, {Hern{\'a}n Gonz{\'a}lez-King} and McGee, {Margaret M.} and Genard, {G{\'e}raldine C.} and Fabiana Geraci and Jamal Ghanam and Subhadip Ghatak and Mahlegha Ghavami and Ghebosu, {Raluca E.} and Gho, {Yong Song} and Sayam Ghosal and Georgios Giamas and Bernd Giebel and Caroline Gilbert and Mario Gimona and Henrique Girao and Ilaria Giusti and Gizzie, {Evan A.} and Sofija Glamoclija and Glass, {Sarah E.} and Jessica Gobbo and Goberdhan, {Deborah C. I.} and Nihar Godbole and Goetz, {Jacky G.} and Olesia Gololobova and Manuel Gomez-Florit and Goncalves, {Jenifer Pendiuk} and Cansu Gorgun and Alessandro Gori and Sabina Gorska and Graner, {Michael W.} and Grau, {Georges E.} and Laura Grech and Greening, {David W.} and Gro{\ss}, {R{\"u}diger M} and Gross, {Julia C.} and Jens Gruber and Alice Gualerzi and Dominic Guanzon and Gudbergsson, {Johann M.} and Guerin, {Coralie L.} and Flora Guerra and Guill{\'e}n, {Maria I.} and Vikramsingh Gujar and Wei Guo and Gupta, {Veer Bala} and Gupta, {Vivek Kumar} and Dakota Gustafson and Edina Gyukity-Sebesty{\'e}n and Patrick H{\"o}lker and Hade, {Mangesh D.} and Hagey, {Daniel W.} and Chungmin Han and Pingping Han and Rikinari Hanayama and Aase Handberg and Edveena Hanser and Masako Harada and Maria Harmati and Harris, {Adrian L.} and Paul Harrison and Harrison, {Rane A.} and Haughey, {Norman J.} and Haynes, {Paul A.} and Mei He and Hargita Hegyesi and An Hendrix and {van Herwijnen}, {Martijn J. C.} and Hill, {Andrew F.} and Hisey, {Colin L.} and Hochberg, {Fred H.} and Hoen, {Esther N. M. Nolte-{\textquoteright}t} and Marija Holcar and Beth Holder and Wolfgang Holnthoner and Harry Holthofer and Hooper, {D. Craig} and Elham Hosseini-Beheshti and Baharak Hosseinkhani and Jane Howard and Howe, {Kathryn L.} and Hoyle, {Nicholas R.} and Jiri Hrdy and Guoku Hu and Yiyao Huang and Veronica Huber and Samo Hudoklin and Antonia Hufnagel and Hulett, {Mark D.} and Stuart Hunt and Vincent Hyenne and Ianni, {Emilio Di} and Dalila Iannotta and Ibrahim, {Ahmed G. E.} and Ibrahim, {Sherif A.} and Seiko Ikezu and Tsuneya Ikezu and Hyungsoon Im and Inal, {Jameel M.} and Aleksandra Inic-Kanada and Marit Inngjerdingen and Yasuo Inoshima and Ivanov, {Alexander R.} and Alena Ivanova and Elena Izquierdo and J{\o}rgensen, {Malene M{\o}ller} and Jackson, {Hannah K.} and Soren Jacobsen and Fernanda Jadue and Naureen Javeed and Jay, {Steven M.} and Muthuvel Jayachandran and Jayasinghe, {Migara K.} and Guido Jenster and Jeppesen, {Dennis K.} and Carmen Jer{\'o}nimo and Linglei Jiang and Jing Jin and Kentaro Jingushi and Dong-Gyu Jo and Joerger-Messerli, {Marianne S.} and Jones, {Jennifer C.} and Jones, {Melissa K.} and {de Jong}, {Olivier G.} and Tijana Jovanovic-Talisman and {Ferrante Jr}, {Anthony W.} and {Coleman Jr}, {Leon G.} and David Juncker and Stephanie Jung and Benjamin Jurek and Marcin Jurga and Verline Justilien and Mehdi Kabani and Raghu Kalluri and Masood Kamali-Moghaddam and Masamitsu Kanada and Taeyoung Kang and Shin-ichi Kano and Maria Kaparakis-Liaskos and Elzbieta Karnas and Antoine Karoichan and Fatah Kashanchi and Kashani, {Sara Assar} and Kashyap, {Namita N.} and Miroslava Katsur and Silvio Kau-Strebinger and Kauffman, {Amy C.} and Sukhbir Kaur and Oksana Kehoe and Kelwick, {Richard J. R.} and Kenari, {Amirmohammad Nasiri} and Kestecher, {Brachyahu M.} and Keulers, {Tom G.} and {Van Keuren-Jensen}, {Kendall R.} and Kasra Khalaj and Delaram Khamari and Ramin Khanabdali and Elena Khomyakova and Amanda Khoo and Kim, {Daniel H.} and Dongin Kim and Kim, {Han Sang} and In-San Kim and Soo Kim and Yohan Kim and Kima, {Peter E.} and Thomas Kislinger and Mikael Klingeborn and Rob Knight and Hiroaki Komuro and Anna Koncz and Timothea Konstantinou and {van der Koog}, Luke and Kooijmans, {Sander A. A.} and Kornek, {Miroslaw T.} and Maja Kosanovic and Enis Kostallari and Koukoulis, {Tiana F.} and Stella Kourembanas and Eva-Maria Kr{\"a}mer-Albers and Veronika Kralj-Iglic and Susanne Krasemann and Krasnodembskaya, {Anna D.} and Krawczynska, {Natalia J.} and Kreft, {Mateja E.} and Kruh-Garcia, {Nicole A.} and Kuehn, {Meta J.} and Kuipers, {Marije E.} and Konxhe Kulaj and Julia Kuligowski and Yumi Kumagai and Ashish Kumar and Saroj Kumar and Sharad Kumar and Meena Kumari and Gabrielis Kundrotas and Kurochkin, {Igor V.} and Masahiko Kuroda and Marzena Kurzawa-Akanbi and Kweskin, {Sasha J.} and Diego Kyburz and Elisa L{\'a}zaro-Ib{\'a}{\~n}ez and Cecilia L{\"a}sser and Jan L{\"o}tvall and Lorincz, {{\'A}kos M.} and Andrew Lai and Lai, {Charles P.} and Saara Laitinen and Solange Landreville and Sigrun Lange and Langevin, {Scott M.} and Marc-Andr{\'e} Langlois and Languino, {Lucia R.} and Joanne Lannigan and Lark, {Daniel S.} and Larregina, {Adriana T.} and Laurent, {Louise C.} and David Laurin and Gregory Lavieu and Charlotte Lawson and {Le Lay}, Soazig and Kevin Leandro and Aur{\'e}lie Ledreux and Changjin Lee and Dong-Sup Lee and Hakho Lee and Heon-Jin Lee and Lee, {Sun Young} and Lee, {Tae Ryong} and Wai-Leng Lee and Iliya Lefterov and Xinhua Lei and Janne Leivo and Quentin Lemaire and {Paes Leme}, {Adriana F.} and Lemon, {Stanley M.} and Metka Lenassi and Lennon, {Kathleen M.} and Stephen Lenzini and Jonathan Leor and Efrat Levy and Bo Li and Guoping Li and Li, {Jiao Jiao} and Qiubai Li and Xinlei Li and Xiuming Liang and Rebecca Lim and Lim, {Sai Kiang} and Tania Limongi and Aija Line and Lins, {Paula Pinela} and Lien Lippens and Guanshu Liu and Alicia Llorente and Longjohn, {Modeline N.} and {van de Loo}, {Fons A. J.} and Lorenowicz, {Magdalena J.} and Aurelio Lorico and Olivier Loudig and Xavier Loyer and Estefan{\'i}a Lozano-Andr{\'e}s and Biao Lu and Quan Lu and Quentin Lubart and Fabrice Lucien and Lunavat, {Taral R.} and Lundberg, {Ludwig Ermann} and Lundy, {David J.} and Luoto, {Jens C.} and Lyden, {David C.} and Andreas M{\"o}ller and M{\"u}ller, {Janis A.} and MacPhee, {Daniel J.} and Maddox, {Adam L.} and Elise Madec and Maga{\~n}a, {Setty M.} and Vasiliki Mahairaki and Mahoney, {My G.} and Harmeet Malhi and Malnou, {C{\'e}cile E.} and Mamand, {Doste R.} and Kenny Man and Mauro Manno and Pierre-Yves Mantel and Tecla Marangon and Eduardo Marb{\'a}n and Antonio Marcilla and Maremanda, {Krishna P.} and Leonid Margolis and Luis Mari{\~n}as-Pardo and Ivica Maric and Mart{\'i}n, {Silvia S{\'a}nchez} and Eduardo Mart{\'i}nez-Mart{\'i}nez and Catherine Martel and Martens-Uzunova, {Elena S.} and Pilar Martin-Duque and Lorena Martin-Jaular and Martinez-Murillo, {Paola A.} and Sarai Martinez-Pacheco and Tania Martins-Marques and Benjamin Mary and Marzan, {Akbar L.} and Andreu Matamoros-Angles and Suresh Mathivanan and Juntaro Matsuzaki and Mayan, {Maria D.} and Carla Mazzeo and Mariama Mbengue and McCann, {Mark J.} and McIlvenna, {Luke C.} and McVey, {Mark J.} and Nicole Meisner-Kober and Maiken Mellergaard and Giorgia Melli and Kerstin Menck and Menjivar, {Nico G.} and Ramkumar Menon and Mentkowski, {Kyle I.} and Miklavcic, {John J.} and Miklosi, {Andras G.} and Bojana Milutinovic and Minciacchi, {Valentina R.} and Mehdi Mirzaei and Shalini Mishra and Mitchell, {Megan I.} and Mizenko, {Rachel R.} and Danilo Mladenovic and Eqbal Mohamadi and Sujata Mohanty and Fatemeh Momen-Heravi and Mondal, {Sujan K.} and Marta Monguio-Tortajada and Jisook Moon and Morandi, {Mattia I.} and Violaine Moreau and Moreira, {Lissette Retana} and Morelli, {Adrian E.} and Mori, {Marcelo A.} and Masahiro Morimoto and Mathilde Mosser and Motaung, {Thabiso E.} and Etienne Moussay and Vera Mugoni and Francois Mullier and Maurizio Muraca and Saravanakumar Murugesan and Luca Musante and Angelo Music{\`o} and Afrodit{\'e} N{\'e}meth and Krisztina N{\'e}meth and Am{\'e}lie Nadeau and Gi-Hoon Nam and Honami Naora and Riccardo Natoli and Muhammad Nawaz and Irina Nazarenko and Ndukaife, {Justus C.} and Christina Nedeva and Peter Nejsum and Inge Nelissen and Christian Neri and Tommaso Neri and Paolo Neviani and Newman, {Lauren A.} and Ng, {Chiew Yong} and {van Niel}, Guillaume and Rienk Nieuwland and Nadezhda Nikiforova and Leonardo Nimrichter and Chitranshi Nitin and Makon-S{\'e}bastien Njock and Daniele No{\"e}l and Alessio Noghero and Nolan, {John P.} and Sam Noppen and {Noren Hooten}, Nicole and Novaes, {Antonio da Silva} and Lorraine O{\textquoteright}Driscoll and Ana O'Loghlen and Takahiro Ochiya and Johannes Oesterreicher and Oh, {Seung W.} and Attila Ol{\'a}h and Martin Olivier and Ong, {Siew Ling} and Angelica Ortiz and Ortiz, {Luis A.} and Ole {\O}stergaard and Osorio, {Omar A.} and Xabier Osteikoetxea and Matias Ostrowski and David Otaegui and Alexander Otahal and Ozawa, {Patricia M. M.} and Ozkocak, {Dilara C.} and Krisztina P{\'a}l{\'o}czi and Roc{\'i}o P{\'e}rez-Gonz{\'a}lez and Pachane, {Bianca C.} and Hafiza Padinharayil and Daan Paget and Jerome Paggetti and Palacio, {Paola C. Loreto} and Pallasch, {Christian P.} and Roberta Palmulli and Bairen Pang and Liliia Paniushkina and Paschalia Pantazi and Lucia Paolini and Papademetrio, {Daniela L.} and Pietro Parisse and Park, {Dong Jun} and Juhee Park and Young-Gyun Park and Patton, {James G.} and Peake, {Nicholas J.} and Pegtel, {D. Michiel} and H{\'e}ctor Peinado and Francesca Perut and Pfaffl, {Michael W.} and Annika Pfeiffer and Phan, {Thanh Kha} and Phinney, {Donald G.} and Phylactou, {Leonidas A.} and Silvia Picciolini and Monika Pietrowska and Max Piffoux and Cl{\'a}udio Pinheiro and Pink, {Ryan C.} and Pleet, {Michelle L.} and Gabriella Pocsfalvi and Poh, {Qi Hui} and {van der Pol}, Edwin and Ganesha Poojary and Poon, {Ivan K. H.} and Giuseppina Poppa and {del Portillo}, {Hernando A.} and Vendula Pospichalova and Shirley Potter and Powell, {Bonita H.} and Powis, {Simon J.} and Ilaria Prada and Indira Prasadam and Christian Preu{\ss}er and Pua, {Heather H.} and Ferdinando Pucci and Florian Puhm and Berta Puig and Lynn Pulliam and Adityas Purnianto and Puutio, {Johanna M. M.} and Quilang, {Rachel C.} and Rabbani, {Piul S.} and Gorjana Rackov and Annalisa Radeghieri and Radu, {Claudia M.} and Raffai, {Robert L.} and Alok Raghav and Mohammad Rahbari and Rahman, {MD Matiur} and Rahman, {MD Mostafizur} and Rai, {Alex J.} and Stefania Raimondo and Sneha Raju and Janusz Rak and Lausonia Ramaswamy and Javier Ramirez-Ricardo and Ramirez, {Marcel I.} and Shikha Rani and Graca Raposo and Rather, {Hilal A.} and Agnieszka Razim and Antonia Reale and Eduardo Reategui and Reddel, {Caroline J.} and Reddy, {Shivakumar K.} and Stephen Redenti and Reed, {Samantha L.} and Neta Regev-Rudzki and Reiners, {Katrin S.} and Natasa Resnik and Rice, {Gregory E.} and Ricklefs, {Franz L.} and Andrea Ridolfi and Kirsi Rilla and Rimmer, {Michael P.} and Roballo, {Kelly C. S.} and Robbins, {Paul D.} and Roberts, {David D.} and Jordi Roca and Rodal, {Avital A.} and Rodrigues-Junior, {Dorival M.} and Rodrigues, {Marcio L.} and Roefs, {Marieke T.} and Rogers, {Russell G.} and Eva Rohde and Tatu Rojalin and Rita Romani and Miriam Romano and Sophie Rome and Rok Romih and Anna Romolo and {De Rossi}, Tatiane and Rouschop, {Kasper M.} and Routenberg, {David A.} and Quentin Roux and Andrew Rowland and {van Royen}, {Martin E.} and Rozo, {Anna{\"i}g J.} and David Rufino-Ramos and Aurelia Rughetti and Russell, {Ashley E.} and Rutter, {Stephanie F.} and Rysmakhanov, {Myltykbay S.} and S{\'a}nchez, {Catherine A.} and Yoel Sadovsky and Reihaneh Safavi-Sohi and Andras Saftics and Ram Sagar and Susmita Sahoo and Saidu, {Nathaniel E. B.} and Julien Saint-Pol and Edison Salas-Huenuleo and Salazar-Puerta, {Ana I.} and Ayesha Saleem and Salekdeh, {Ghasem Hosseini} and Witwer, {Kenneth W.} and Clotilde Thery",

note = "Copyright the Author(s) 2024. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher. A correction exists for this article, the current version has been updated. The correction can be found at doi: 10.1002/jev2.12451",

year = "2024",

month = feb,

doi = "10.1002/jev2.12404",

language = "English",

volume = "13",

pages = "1--84",

journal = "Journal of Extracellular Vesicles",

issn = "2001-3078",

publisher = "Taylor & Francis",

number = "2",

}

Welsh, JA, Goberdhan, DCI, O’Driscoll, L, Buzas, EI, Blenkiron, C, Bussolati, B, Cai, H, Di Vizio, D, Driedonks, TAP, Erdbrügger, U, Falcon-Perez, JM, Fu, QL, Hill, AF, Lenassi, M, Lim, SK, Mahoney, MG, Mohanty, S, Möller, A, Nieuwland, R, Ochiya, T, Sahoo, S, Torrecilhas, AC, Zheng, L, Zijlstra, A, Abuelreich, S, Bagabas, R, Bergese, P, Bridges, EM, Brucale, M, Burger, D, Carney, RP, Cocucci, E, Crescitelli, R, Hanser, E, Harris, AL, Haughey, NJ, Hendrix, A, Ivanov, AR, Jovanovic-Talisman, T, Kruh-Garcia, NA, Faustino, VKL, Kyburz, D, Lässer, C, Lennon, KM, Lötvall, J, Maddox, AL, Martens-Uzunova, ES, Mizenko, RR, Newman, LA, Ridolfi, A, Rohde, E, Rojalin, T, Rowland, A, Saftics, A, Sandau, US, Saugstad, JA, Shekari, F, Swift, S, Ter-Ovanesyan, D, Tosar, JP, Useckaite, Z, Valle, F, Varga, Z, van der Pol, E, van Herwijnen, MJC, Wauben, MHM, Wehman, AM, Williams, S, Zendrini, A, Zimmerman, AJ, MISEV Consortium, Li, G, Witwer, KW & Thery, C 2024, 'Minimal information for studies of extracellular vesicles (MISEV2023): from basic to advanced approaches', Journal of Extracellular Vesicles, vol. 13, no. 2, e12404, pp. 1-84. https://doi.org/10.1002/jev2.12404

TY - JOUR

T1 - Minimal information for studies of extracellular vesicles (MISEV2023)

T2 - from basic to advanced approaches

AU - Welsh, Joshua A.

AU - Goberdhan, Deborah C. I.

AU - O’Driscoll, Lorraine

AU - Buzas, Edit I.

AU - Blenkiron, Cherie

AU - Bussolati, Benedetta

AU - Cai, Houjian

AU - Di Vizio, Dolores

AU - Driedonks, Tom A.P.

AU - Erdbrügger, Uta

AU - Falcon-Perez, Juan M.

AU - Fu, Qing Ling

AU - Hill, Andrew F.

AU - Lenassi, Metka

AU - Lim, Sai Kiang

AU - Mahoney, Mỹ G.

AU - Mohanty, Sujata

AU - Möller, Andreas

AU - Nieuwland, Rienk

AU - Ochiya, Takahiro

AU - Sahoo, Susmita

AU - Torrecilhas, Ana C.

AU - Zheng, Lei

AU - Zijlstra, Andries

AU - Abuelreich, Sarah

AU - Bagabas, Reem

AU - Bergese, Paolo

AU - Bridges, Esther M.

AU - Brucale, Marco

AU - Burger, Dylan

AU - Carney, Randy P.

AU - Cocucci, Emanuele

AU - Crescitelli, Rossella

AU - Hanser, Edveena

AU - Harris, Adrian L.

AU - Haughey, Norman J.

AU - Hendrix, An

AU - Ivanov, Alexander R.

AU - Jovanovic-Talisman, Tijana

AU - Kruh-Garcia, Nicole A.

AU - Faustino, Vroniqa Ku’ulei Lyn

AU - Kyburz, Diego

AU - Lässer, Cecilia

AU - Lennon, Kathleen M.

AU - Lötvall, Jan

AU - Maddox, Adam L.

AU - Martens-Uzunova, Elena S.

AU - Mizenko, Rachel R.

AU - Newman, Lauren A.

AU - Ridolfi, Andrea

AU - Rohde, Eva

AU - Rojalin, Tatu

AU - Rowland, Andrew

AU - Saftics, Andras

AU - Sandau, Ursula S.

AU - Saugstad, Julie A.

AU - Shekari, Faezeh

AU - Swift, Simon

AU - Ter-Ovanesyan, Dmitry

AU - Tosar, Juan P.

AU - Useckaite, Zivile

AU - Valle, Francesco

AU - Varga, Zoltan

AU - van der Pol, Edwin

AU - van Herwijnen, Martijn J. C.

AU - Wauben, Marca H. M.

AU - Wehman, Ann M.

AU - Williams, Sarah

AU - Zendrini, Andrea

AU - Zimmerman, Alan J.

AU - MISEV Consortium

AU - Ahmad, Samar

AU - Ahmed, Dina A. K.

AU - Ahmed, Sarah H.

AU - Aikawa, Elena

AU - Akbar, Naveed

AU - Akiyoshi, Kazunari

AU - Al-Adra, David P.

AU - Al-Masawa, Maimonah E.

AU - Albanese, Manuel

AU - Alberro, Ainhoa

AU - Alcaraz, María José

AU - Alexander-Brett, Jen

AU - Alexander, Kimberley L.

AU - Ali, Nilufar

AU - Alibhai, Faisal J.

AU - Allelein, Susann

AU - Allenby, Mark C.

AU - Almeida, Fausto

AU - de Almeida, Luis Pereira

AU - Almousa, Sameh W.

AU - Altan-Bonnet, Nihal

AU - Altei, Wanessa F.

AU - Alvarez-Llamas, Gloria

AU - Alvarez, Cora L.

AU - An, Hyo Jung

AU - Anand, Krishnan

AU - Andaloussi, Samir E. L.

AU - Anderson, Johnathon D.

AU - Andriantsitohaina, Ramaroson

AU - Ansari, Khairul I.

AU - Anselmo, Achille

AU - Antoniou, Anna

AU - Aqil, Farrukh

AU - Arab, Tanina

AU - Archer, Fabienne

AU - Arif, Syrine

AU - Armstrong, David A.

AU - Arntz, Onno J.

AU - Arsène, Pierre

AU - Arteaga-Blanco, Luis

AU - Asokan, Nandini

AU - Aspelin, Trude

AU - Atkin-Smith, Georgia K.

AU - Aubert, Dimitri

AU - Ayyar, Kanchana K.

AU - Azlan, Maryam

AU - Azoidis, Ioannis

AU - Bécot, Anaïs

AU - Bach, Jean Marie

AU - Bachurski, Daniel

AU - Bae, Seoyoon

AU - Bagge, Roger Olofsson

AU - Baj-Krzyworzeka, Monika

AU - Balaj, Leonora

AU - Balbi, Carolina

AU - van Balkom, Bas W. M.

AU - Ballal, Abhijna R.

AU - Bano, Afsareen

AU - Banzet, Sébastien

AU - Bare, Yonis

AU - Barile, Lucio

AU - Barman, Bahnisikha

AU - Barranco, Isabel

AU - Barreca, Valeria

AU - Bart, Geneviève

AU - Barteneva, Natasha S.

AU - Basso, Manuela

AU - Batish, Mona

AU - Bauer, Natalie R.

AU - Baxter, Amy A.

AU - Bazié, Wilfried W.

AU - Bazzan, Erica

AU - Beaumont, Joel E. J.

AU - Bebawy, Mary

AU - Bebelman, Maarten P.

AU - Bedina-Zavec, Apolonija

AU - Beetler, Danielle J.

AU - Beke-Somfai, Tamás

AU - Belleannée, Clémence

AU - Benedikter, Birke J.

AU - Benediktsdóttir, Berglind E.

AU - Berardi, Anna C.

AU - Bergamelli, Mathilde

AU - Bertolini, Irene

AU - Bhattacharyya, Asima

AU - Bhattacharyya, Suvendra N.

AU - Biller, Steven J.

AU - Billottet, Clotilde

AU - Bissler, John J.

AU - Blanc-Brude, Olivier

AU - Blijdorp, Charles J.

AU - Bobis-Wozowicz, Sylwia

AU - Bodart-Santos, Victor

AU - Bodnár, Bernadett R.

AU - Boilard, Eric

AU - Boireau, Wilfrid

AU - Bokun, Vladimir

AU - Bollard, Stephanie M.

AU - Bollini, Sveva

AU - Bongiovanni, Antonella

AU - Bongiovanni, Laura

AU - Bonifay, Amandine

AU - Boppart, Marni D.

AU - Borràs, Francesce

AU - Bosch, Steffi

AU - Boselli, Daniela

AU - Bottini, Massimo

AU - Bouffard, Jeff

AU - Boulanger, Chantal M.

AU - Boutros, Paul C.

AU - Boyadjian, Oscar

AU - Boysen, Anders T.

AU - Bozkurt, Batuhan T.

AU - Bramich, Kyle P.

AU - Braun, Fabian

AU - Bravo-Miana, Rocío Del Carmen

AU - Breakefield, Xandra O.

AU - Brenna, Santra

AU - Brennan, Kieran

AU - Brennan, Meadhbh

AU - Breyne, Koen

AU - Brigstock, David R.

AU - Brisson, Alain R.

AU - Brodie, Chaya

AU - Bruno, Katelyna

AU - Bucci, Cecilia

AU - Buch, Shilpa

AU - Buck, Amyh

AU - Bukva, Mátyás

AU - Bulte, Jeff W. M.

AU - Buratta, Sandra

AU - Burgy, Olivier

AU - Burnier, Julia V.

AU - Burrows, Kaiping

AU - Busatto, Sara

AU - Buzas, Krisztina

AU - Byrd, J. Brian

AU - Cáceres-Verschae, Albano

AU - Caires, Hugo R.

AU - Campos-Silva, Carmen

AU - Camussi, Giovanni

AU - de Candia, Paola

AU - Carceller, Carmen

AU - Fernandez-Becerra, Carmen

AU - Carrasco, Alexis G.Murillo

AU - Carter, David R. F.

AU - Cavallaro, Sara

AU - Cavallero, Serena

AU - Cavallero, Sophie

AU - Cerda-Troncoso, Cristóbal

AU - Chen, Hao

AU - Chen, Qiang

AU - Chen, Shuai

AU - Chen, Siyu

AU - Chen, Yunxi

AU - Cheng, Lesley

AU - Chernyshev, Vasiliy S.

AU - Chetty, Venkatesh Kumar

AU - Chitti, Sai V.

AU - Cho, Ssang-Goo

AU - Cho, Yoon-Kyoung

AU - Choi, Byeong Hyeon

AU - Chutipongtanate, Somchai

AU - Cicardi, Maria Elena

AU - Cifuentes-Ruis, Anna

AU - Ciullo, Alessandra

AU - Clayton, Aled

AU - Cleary, Jacob A.

AU - Cocozza, Federico

AU - Cocucci, Emanuele

AU - Coffey, Robert J.

AU - Collino, Federica

AU - Colombo, Federico

AU - Colosetti, Pascal

AU - Compañ-Bertomeu, Alvaro

AU - Constanzo, Julie

AU - Corbeil, Denis

AU - Cordeiro-da-Silva, Anabela

AU - Costa, Júlia

AU - Couch, Yvonne

AU - Courageux, Yvan

AU - Coutant, Kelly

AU - Coyle, Beth

AU - Crescitelli, Rossella

AU - Cretich, Marina

AU - Cronemberger-Andrade, André

AU - Crossland, Rachel E.

AU - Cucher, Marcela A.

AU - Czystowska-Kuzmicz, Malgorzata

AU - D'Acunzo, Pasquale

AU - D'Agnano, Igea

AU - D'Agostino, Vito G.

AU - D'Angelo, Gisela

AU - D'Arrigo, Daniele

AU - D'Souza-Schorey, Crislyn

AU - Dagur, Raghubendra S.

AU - Danielson, Kirsty M.

AU - Das, Saumya

AU - Dauphin, Thibaud

AU - Davidson, Sean M.

AU - Davies, Owen G.

AU - Davies, Rebecca L.

AU - Davis, Chelsea N.

AU - Deep, Gagan

AU - Degosserie, Jonathan

AU - Van Delen, Mats

AU - Deliwala, Vatsal

AU - Dellar, Elizabeth R.

AU - Van Deun, Jan

AU - Dev, Apurba

AU - Deville, Sarah

AU - Devitt, Andrew

AU - Dhondt, Bert

AU - Dieterich, Lothar C.

AU - Dittmer, Dirk P.

AU - Dobosh, Brian

AU - Dobra, Gabriella

AU - Dogra, Navneet

AU - Dohi, Eisuke

AU - Dolo, Vincenza

AU - Domashevich, Timothy V.

AU - Dominici, Massimo

AU - Dong, Liang

AU - Doré, Etienne

AU - Dragovic, Rebecca A.

AU - Driedonks, Tom A. P.

AU - Drittanti, Lila

AU - Droste, Marvin

AU - Duan, Wei

AU - Durmaz, Esmahan

AU - Dutta, Suman

AU - Eguchi, Takanori

AU - Eichenberger, Ramon M.

AU - Eitan, Erez

AU - Ekström, Karin

AU - Eldh, Maria

AU - Elie-Caille, Celine

AU - Enciso-Martinez, Agustin

AU - Erdbrügger, Uta

AU - Esmaeili, Rezvan

AU - Ettelaie, Camille

AU - Försönits, András I.

AU - Fabbri, Muller

AU - Falasca, Marco

AU - Falcon-Perez, Juan M.

AU - Fan, Hongkuan

AU - Fatima, Farah

AU - Faustino, Vroniqa Ku’ulei-Lyn

AU - Fazeli, Alireza

AU - Fernández-Rhodes, María

AU - Fernandez-Prada, Christopher

AU - Ferraro, Mariola J.

AU - Ferreira, Joao N.

AU - Ferreira, Rafaela F.

AU - Figueroa-Hall, Leandra K.

AU - Figueroa-Valdés, Aliosha I.

AU - Fioretti, Paolo V.

AU - Flenady, Sabine

AU - Flores-Bellver, Miguel

AU - Fok, Ellis K.

AU - Fonseka, Pamali

AU - Forbes, Karen

AU - Ford, Verity J.

AU - Fornaguera, Cristina

AU - Forte, Dorian

AU - Forte, Stefano

AU - Fortunato, Orazio

AU - Franklin, Jeffrey L.

AU - Freitas, Daniela

AU - Frelet-Barrand, Annie

AU - Fu, Qing-Ling

AU - Fujita, Yu

AU - Gärtner, Kathrin

AU - Görgens, André

AU - Gabriel, Áurea M.

AU - Gabrielli, Martina

AU - Gabrielsson, Susanne

AU - Galinsoga, Alicia

AU - Galisova, Andrea

AU - Gamage, Teena K. J. B.

AU - Gao, Yingtang

AU - Garcia-Contreras, Marta

AU - Garcia, M. Madhy Garcia

AU - Garcia, Maria Noé

AU - Gargiulo, Ernesto

AU - Garibotti, Hernán González-King

AU - McGee, Margaret M.

AU - Genard, Géraldine C.

AU - Geraci, Fabiana

AU - Ghanam, Jamal

AU - Ghatak, Subhadip

AU - Ghavami, Mahlegha

AU - Ghebosu, Raluca E.

AU - Gho, Yong Song

AU - Ghosal, Sayam

AU - Giamas, Georgios

AU - Giebel, Bernd

AU - Gilbert, Caroline

AU - Gimona, Mario

AU - Girao, Henrique

AU - Giusti, Ilaria

AU - Gizzie, Evan A.

AU - Glamoclija, Sofija

AU - Glass, Sarah E.

AU - Gobbo, Jessica

AU - Goberdhan, Deborah C. I.

AU - Godbole, Nihar

AU - Goetz, Jacky G.

AU - Gololobova, Olesia

AU - Gomez-Florit, Manuel

AU - Goncalves, Jenifer Pendiuk

AU - Gorgun, Cansu

AU - Gori, Alessandro

AU - Gorska, Sabina

AU - Graner, Michael W.

AU - Grau, Georges E.

AU - Grech, Laura

AU - Greening, David W.

AU - Groß, Rüdiger M

AU - Gross, Julia C.

AU - Gruber, Jens

AU - Gualerzi, Alice

AU - Guanzon, Dominic

AU - Gudbergsson, Johann M.

AU - Guerin, Coralie L.

AU - Guerra, Flora

AU - Guillén, Maria I.

AU - Gujar, Vikramsingh

AU - Guo, Wei

AU - Gupta, Veer Bala

AU - Gupta, Vivek Kumar

AU - Gustafson, Dakota

AU - Gyukity-Sebestyén, Edina

AU - Hölker, Patrick

AU - Hade, Mangesh D.

AU - Hagey, Daniel W.

AU - Han, Chungmin

AU - Han, Pingping

AU - Hanayama, Rikinari

AU - Handberg, Aase

AU - Hanser, Edveena

AU - Harada, Masako

AU - Harmati, Maria

AU - Harris, Adrian L.

AU - Harrison, Paul

AU - Harrison, Rane A.

AU - Haughey, Norman J.

AU - Haynes, Paul A.

AU - He, Mei

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N1 - Copyright the Author(s) 2024. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher. A correction exists for this article, the current version has been updated. The correction can be found at doi: 10.1002/jev2.12451

PY - 2024/2

Y1 - 2024/2

N2 - Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.

AB - Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.

KW - ectosomes

KW - exosomes

KW - extracellular particles

KW - extracellular vesicles

KW - guidelines

KW - microparticles

KW - microvesicles

KW - minimal information requirements

KW - MISEV

KW - reproducibility

KW - rigor

KW - standardisation

UR - http://www.scopus.com/inward/record.url?scp=85183359756&partnerID=8YFLogxK

U2 - 10.1002/jev2.12404

DO - 10.1002/jev2.12404

M3 - Article

C2 - 38326288

AN - SCOPUS:85183359756

SN - 2001-3078

VL - 13

SP - 1

EP - 84

JO - Journal of Extracellular Vesicles

JF - Journal of Extracellular Vesicles

IS - 2

M1 - e12404

ER -

Minimal information for studies of extracellular vesicles (MISEV2023): from basic to advanced approaches

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